Great diversity of Piroplasmida in Equidae in Africa and Europe, including potential new species

Dahmana, Handi; Amanzougaghene, Nadia; Davoust, Bernard; Normand, Thomas; Carette, Olivier; Demoncheaux, Jean‐Paul; Mulot, Baptiste; Fabrizy, Bernard; Scandola, Pierre; Chik, Makhlouf; Fénollar, Florence; Mediannikov, Oleg

doi:10.1016/j.vprsr.2019.100332

Cited by 24 publications

(34 citation statements)

References 35 publications

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“…"Africa" previously detected in African horses in Senegal and Chad (Fig. 6) [50]. As far as we know, Theileria sp.…”

Section: Discussionmentioning

confidence: 65%

“…Quantitative PCR (qPCR) was performed in all extracted DNA samples (equines and their vectors) for multipathogen DNA screening using genus-specific primers and probes targeting the 5.8S rRNA gene of Piroplasmida [50], the 23S rRNA gene of Anaplasmataceae [51], the gltA gene Rickettsia sp. [52], the 16S rRNA gene Borrelia sp.…”

Section: -Molecular Detection Of Pathogen Dna In Equines and Their Vmentioning

confidence: 99%

“…and Borrelia spp. 1100 bp of the 18S rRNA gene, a 520 bp fragment of the 23S rRNA gene and a 1200 bp of 16S rRNA gene were used; respectively[50,51,53]. The PCR reactions were performed on a Thermocycler (Applied Biosystem, Paris, France) using the AmpliTaq Gold® 360 Master Mix (ThermoFisher Scientific, USA) according to the manufacturer's recommendations.…”

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confidence: 99%

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Molecular Characterization of Some Equine Vector-Borne Pathogens and Identification of Their Vectors in Egypt

Abdullah¹,

Aboelsoued²,

Farag³

et al. 2020

Preprint

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Background Equine vector borne diseases (EVBD) have been considered emerging and reemerging diseases transmitted by arthropods and most of these diseases have zoonotic concern. This study was designed to screen EVBD in equines and their vectors using molecular analyses and identify vectors by MALDI-TOF and molecular techniques.Methods A total of 335 blood samples were collected from apparently healthy equines (320 from horses and 15 from donkeys) from Cairo and Beni-Suef provinces in Egypt. A total of 166 arthropods (105 sucking flies and 61 ticks) were collected from the same animals. MALDI-TOF and molecular techniques were used to confirm the findings of morphological identification of vector. Quantitative PCR and Standard PCR coupled with sequencing were performed in equines and vectors DNA for screening multiple pathogens.Results MALDI-TOF and molecular techniques confirmed that Hippoposca equina (louse fly), Rhipicephalus annulatus (Rh. annulatus) and Rh. microplus ixodid ticks were found. In vectors, we identified Anaplasma marginale (A. marginale; 1.6%), A. platys-like (1.6%) and a new Ehrlichia sp. (4.9%) in Rh. microplus, while Ehrlichia rustica (E. rustica) was found in Rh. microplus and Rh. annulatus. Likewise, Borrelia theileri was identified in Rh. microplus (3.3%). For H. equina, Anaplasma and Borrelia sp. DNA were detected by qPCR only. In equines, A. marginale (0.6%), A. ovis (0.6%) and Theileria ovis (T. ovis; 0.6%) were found in donkeys. In horses, T. equi (1.2%) and a new Theileria sp. Africa (2.7%) were identified.Conclusions For the first time, we reported here the presence of Rh. microplus as a competent tick for Rh. annulatus in Egypt using MALDI-TOF and molecular identification. To the best of our knowledge, we provided the first detection of different pathogens as A. marginale, A. platys-like, E. rustica, new Ehrlichia sp., B. theileri in Rh. microplus, A. marginale, A. ovis and T. ovis in donkeys and a new Theileria sp. Africa in horses in Egypt.

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“…"Africa" previously detected in African horses in Senegal and Chad (Fig. 6) [50]. As far as we know, Theileria sp.…”

Section: Discussionmentioning

confidence: 65%

Section: -Molecular Detection Of Pathogen Dna In Equines and Their Vmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Characterization of Some Equine Vector-Borne Pathogens and Identification of Their Vectors in Egypt

Abdullah¹,

Aboelsoued²,

Farag³

et al. 2020

Preprint

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“…Few reports describing T. equi infection in Africa include genotyping. Reports from the region describe T. equi 18S rRNA genotypes A, C and D in neighboring Chad [27], as well as in Tunisia [28]; genotypes A and D in Congo and Senegal [26]; and genotypes B and D in Sudan [29].…”

Section: Discussionmentioning

confidence: 99%

Genetic Characterization of Piroplasms in Donkeys and Horses from Nigeria

Idoko

Tirosh-Levy

Mazuz³

et al. 2020

Animals

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Equine piroplasmosis (EP) is a tick-borne disease of equids, caused by the two haemoprotozoal parasites: Theileria equi and Babesia caballi. Nigeria constitutes a major crossroads of animal transport in West Africa and may serve as a factor in EP dissemination in the region. The study aim was to characterize EP parasites in donkeys and horses in northern Nigeria using a molecular approach. Blood was collected from 57 donkeys and 47 horses. EP infection was detected and characterized by polymerase chain reaction (PCR). Twenty five donkeys (43.8%) were infected with T. equi, five (8.8%) with B. caballi, three (5.3%) with dual infections. Four horses (8.5%) were infected by T. equi and none by B. caballi. Four of the five known T. equi 18S rRNA genotypes (A, B, C and D) were identified. Theileria equi ema-1 and ema-2 genes were amplified in only 2 and 10 samples, respectively, showing no genetic variation. All B. caballi isolates were classified as rap-1 genotype A1. Twenty-two (42.3%) of the donkeys were positive for anti-T. equi antibodies and 29 (55.8%) were positive for anti-B. caballi antibodies, using immunofluorescence antibody test (IFAT). The study results demonstrate high genetic variation within T. equi parasites, suggesting that donkeys may be reservoirs of EP parasites in West Africa.

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“…The primers were designed to target a conserved region that encoded ribosomal 18S rRNA (969 bp) and 238S rRNA (485 bp) to detect the DNA of Babesia [18] and Anaplasma [19], respectively ( Table 1). Polymerase chain reaction (PCR) assays were performed in an automated BIO-RAD Thermal Cycler (BIO-RAD, Singapore) using one PCR master mix (GeneDireX, Taiwan).…”

Section: Molecular Proceduresmentioning

confidence: 99%

Molecular detection of tick-borne pathogens in cattle from Saudi Arabia

Alanazi

Alouffi

Alshahrani

et al. 2020

Preprint

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Background: Babesiosis and anaplasmosis are tick-borne diseases that affect the health of cattle and may also be responsible for major economic losses. This study aimed to detect the presence of the causative agents of babesiosis and anaplasmosis among cattle in different provinces in Saudi Arabia and to characterise their species genetically. Methods: A total of 362 blood samples were taken from cattle in four regions (Riyadh, Al-Kharj, Al-Hasa and Al-Qassim) of Saudi Arabia and were molecularly screened by polymerase chain reaction (PCR) of partial 18S rRNA and 23S rRNA genes to detect Babesia and Anaplasma species. Results: The overall prevalence of Babesia and Anaplasma in cattle was 2.49% and 5.80%, respectively. Theileria annulata (T. annulata) and Theileria ovis (T. ovis) were found in seven (1.93%) and two (0.55%) of the 362 samples, respectively. Anaplasma ovis (A. ovis) was detected in 21 (5.80%) of 362 samples. Likewise, four of the cattle were found to be coinfected with more than one pathogen (1.10%). All cattle samples tested negative for other species of Babesia, Theileria and Anaplasma. Conclusions: The presence of T. ovis and A. ovis has been reported in Saudi Arabia. Here, the presence of two novel genotypes of T. annulata is also reported in Saudi Arabian cattle. Further molecular surveys on larger numbers of samples from the entire country are needed to correctly address the prevalence and geographical distribution of tick-borne disease.

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Great diversity of Piroplasmida in Equidae in Africa and Europe, including potential new species

Cited by 24 publications

References 35 publications

Molecular Characterization of Some Equine Vector-Borne Pathogens and Identification of Their Vectors in Egypt

Molecular Characterization of Some Equine Vector-Borne Pathogens and Identification of Their Vectors in Egypt

Genetic Characterization of Piroplasms in Donkeys and Horses from Nigeria

Molecular detection of tick-borne pathogens in cattle from Saudi Arabia

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