Gravity spun polycaprolactone fibres for soft tissue engineering: Interaction with fibroblasts and myoblasts in cell culture

Williamson, Matthew R.; Adams, Eric F.; Coombes, Allan G.A.

doi:10.1016/j.biomaterials.2005.06.018

Cited by 156 publications

(44 citation statements)

References 23 publications

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“…22 The mechanism of cold drawing has also been reported for PCL (M w = 115 000 g/mol) where a fiber elongation of 500% leads to a 5-fold increase of the tensile strength (from 8 to 43 MPa) and a 3-fold increase of the elastic modulus (from 0.1 to 0.3 GPa). 23 In our proposed explanatory model PCL fills the accessible micropores and the surface flaws of lamellae (Figure 7). If a load is applied to the sample by bending (Figure 7a) or compression (Figure 7b), stress develops inside the ceramic lamellae, and stress concentrations are located in the vicinity of surface flaws and micropores.…”

Section: Discussionmentioning

confidence: 99%

Mechanical Properties of Porous β-Tricalcium Phosphate Composites Prepared by Ice-Templating and Poly(ε-caprolactone) Impregnation

Flauder

Sajzew

Müller

2014

ACS Appl. Mater. Interfaces

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In this study ceramic scaffolds of the bioresorbable and osteoconductive bioceramic β-tricalcium phosphate (β-TCP) were impregnated with the bioresorbable and ductile polymer poly(ε-caprolactone) (PCL) to investigate the influence of the impregnation on the mechanical properties of the porous composites. The initial β-TCP scaffolds were fabricated by the ice-templating method and exhibit the typical morphology of aligned, open, and lamellar pores. This pore morphology seems to be appropriate for applications as bone replacement material. The macroporosity of the scaffolds is mostly preserved during the solution-mediated PCL impregnation as the polymer was added only in small amounts so that only the micropores of β-TCP lamellae were infiltrated and the surface of the lamellae were coated with a thin film. Composite scaffolds show a failure behavior with brittle and plastic contributions, which increase their damage tolerance, in contrast to the absolutely brittle behavior of pure β-TCP scaffolds. The energy consumption during bending and compression load was increased in the impregnated scaffolds by (a) elastic and plastic deformation of the introduced polymer, (b) drawing and formation of PCL fibrils which bridge micro- and macrocracks, and (c) friction of ceramic debris still glued together by PCL. PCL addition also increased the compressive and flexural strength of the scaffolds. An explanatory model for this strength enhancement was proposed that implicates the stiffening of cold-drawn PCL present in surface flaws and micropores.

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Section: Discussionmentioning

confidence: 99%

Mechanical Properties of Porous β-Tricalcium Phosphate Composites Prepared by Ice-Templating and Poly(ε-caprolactone) Impregnation

Flauder

Sajzew

Müller

2014

ACS Appl. Mater. Interfaces

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“…It is well known in literature that gelatine promotes cell adhesion (Williamson et al 2006;Huang et al 2005) and in this paper the cellular response towards the mTGase mediated modified protein blended with a polysaccharide was evaluated. The presence of viable cells on the samples was evident and a double layer after 48 h from the seeding was detectable.…”

Section: Gelatinementioning

confidence: 99%

Transglutaminase Reactivity with Gelatine: Perspective Applications in Tissue Engineering

et al. 2006

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Gelatine was crosslinked by means of an enzymatic treatment using tissue transglutaminase (tTGase) (Sigma) and microbial transglutaminase (mTGase) (Ajinomoto) which catalyses the formation of isopeptide bonds between the gamma-carbonyl group of a glutamine residue and the epsilon-amino group of a lysine residue. The reaction is an interesting alternative to the traditional glutaraldehyde crosslinking, which has several drawbacks (e.g., in medical application) due to the toxicity of the chemical reagent. To further investigate the possibility to utilize the modified protein for tissue engineering application, TGase crosslinked gelatine was incorporated in a gellan matrix, a polysaccharide, to enhance the stability in aqueous media. Films obtained by casting were characterized by thermal analysis, chemical imaging, swelling behaviour and cell adhesion.

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“…20 Nanofibers can be produced from materials that are biocompatible and biodegradable, such as poly-ε-caprolactone (PCL), 21 poly(lactic-co-glycolic) acid (PLGA), 21 and chitosan. 22 PCL has frequently been chosen for electrospinning, because it is a US Food and Drug Administration (FDA)-approved material and has been shown to support the attachment and growth of chondrocytes, 23 osteoblasts, 24,25 smooth muscle cells, 26 fibroblasts, 24,[27][28][29] myoblasts, 30 and mesenchymal stem cells (MSCs). 31 PCL appears to be a suitable material for scaffold preparation for reparative surgery.…”

mentioning

confidence: 99%

Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution

Plencner

Prosecká

Rampichová³

et al. 2015

IJN

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Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2–3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

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Gravity spun polycaprolactone fibres for soft tissue engineering: Interaction with fibroblasts and myoblasts in cell culture

Cited by 156 publications

References 23 publications

Mechanical Properties of Porous β-Tricalcium Phosphate Composites Prepared by Ice-Templating and Poly(ε-caprolactone) Impregnation

Mechanical Properties of Porous β-Tricalcium Phosphate Composites Prepared by Ice-Templating and Poly(ε-caprolactone) Impregnation

Transglutaminase Reactivity with Gelatine: Perspective Applications in Tissue Engineering

Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution

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Gravity spun polycaprolactone fibres for soft tissue engineering: Interaction with fibroblasts and myoblasts in cell culture

Cited by 156 publications

References 23 publications

Mechanical Properties of Porous β-Tricalcium Phosphate Composites Prepared by Ice-Templating and Poly(ε-caprolactone) Impregnation

Mechanical Properties of Porous β-Tricalcium Phosphate Composites Prepared by Ice-Templating and Poly(ε-caprolactone) Impregnation

Transglutaminase Reactivity with Gelatine: Perspective Applications in Tissue Engineering

Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-&epsilon;-caprolactone nanofibers functionalized with thrombocyte-rich solution

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Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution