Gravitational field-flow fractionation for the characterisation of active dry wine yeast

Study of the growth rate of Saccharomyces cerevisiae strains using wheat starch granules as support for yeast immobilization monitoring by sedimentation/steric field-flow fractionation

“…The large granules have diameters of about 14 lm and the small ones of about 6 lm. It is also known Sanz et al, 2001) that S. cerevisiae single cells have a diameter of about 4 lm, while the diploid cells (or clusters and chains) show a size distribution of roughly 5-9 lm. It is obvious that, according to their size, yeast cells could be immobilized on wheat starch granules.…”

“…Field flow fractionation (FFF) is an analytical simple and accurate technique with low cost of operation. It is used not only for the characterization but also for the separation of colloids with biological interesting such as yeast cells Sanz, Cardot, Battu, & Galceran, 2002;Sanz, Puignou, Reschiglian, & Galceran, 2001), starch granules (Farmakis, Sakellaraki, Koliadima, Gavril, & Karaiskakis, 2000;Farmakis, Koliadima, & Karaiskakis, 2002a;Farmakis, Koliadima, & Karaiskakis, 2002b) and proteins (Arfvidsson & Wahlund, 2003) as well as for medical research (Leger, Liagre, Cardot, Beneytout, & Battu, 2004), etc. It is a one-phase chromatographic technique in which an externally adjusted force field is applied to the suspended particles under motion in a channel (Karaiskakis, Myers, Caldwell, & Giddings, 1981;Giddings, 1993).…”

Size Determination of Pollens Using Gravitational and Sedimentation Field-Flow Fractionation

“…Particles are driven towards the channel outlet at different velocities depending on their sizes. GrFFF has been employed for separation of various particulate materials including red blood cells, [9][10][11] parasites, 12,13 wine yeast, 14,15 wheat starch, 16,17 inorganic particles, [18][19][20][21] and particles of biological interest.…”

Fast “hyperlayer” separation development in sedimentation field flow fractionation☆

“…In order to establish the basic characteristics of separation comparison, elutions were performed with certified particles used as standards. Field flow fractionation [9], follows a constant but relatively confidential development, and is now well described [4][5][6][10][11][12][13][14][15].…”