Gravimetric Estimation of Proteins Precipitated by Trichloroacetic Acid

Hoch, Frederic L.; Vallée, Bert L.

doi:10.1021/ac60074a029

Cited by 62 publications

(23 citation statements)

References 20 publications

(16 reference statements)

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“…Protein concentrations were determined gravimetrically after precipitation with trichloracetic acid and drying at 104° (Hoch and Vallee, 1953). The absorbance at 250 mg served as alternate method for estimating the concentration of metallothionein (Kági and Vallee, 1961).…”

Section: Methodsmentioning

confidence: 99%

Isolation and Some Properties of Human Metallothionein^*

Pulido¹,

Kägi²,

Vallée³

1966

Biochemistry

324

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Section: Methodsmentioning

confidence: 99%

Isolation and Some Properties of Human Metallothionein^*

Pulido¹,

Kägi²,

Vallée³

1966

Biochemistry

324

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“…Protein concentrations were determined from the absorbance at 278 mp using molar absorptivities for native and succinylcarboxypeptidase of 6.32 and 6.47 X IO4 1. mole-' cm-1, respectively,as determined gravimetrically, after 10 % trichloracetic acid precipitation followed by drying at 104" (Hoch and Vallee, 1953). A Beckman Model DU spectrophotometer was used throughout.…”

Section: Methodsmentioning

confidence: 99%

Physicochemical Studies of Carboxypeptidase A Derivatives. II. N-Succinylcarboxypeptidase^*

Bethune¹,

Ulmer²,

Vallée³

1967

Biochemistry

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Previous studies have shown that modification of active center tyrosyl residues of carboxypeptidase A with acetylimidazole markedly alters catalytic activity without discernible change in protein structure. kcetylation of both tyrosyl and lysyl residues with acetic anhydride induces similar catalytic changes but concomitantly alters protein structure (Bethune, J. L., Ulmer, D. D., and Vallee, B. L., Biochemistry 3, 1764 (1964)). The consequences of modifying amino groups only now have been assessed in an N-succinyl derivative of the enzyme. The structural stability of N-succinylcarboxypeptidase enzyme as a function of p H does not B ovine pancreatic carboxypeptidase A has proven a useful system for assessing relationships of protein structure and composition to enzymatic function. The enzyme exhibits remarkable alterations in catalytic rates, specificity, or both when subjected to a wide range of inorganic substitutions (Coleman and Vallee, 1960), and organic modifications such as acetylation (Simpson et ut., 1963;Riordan and Vallee, 1963), acylation with dicarboxylic acid anhydrides (Riordan and Vallee, 1964), iodination (Simpson and Vallee, 1966), coupling with 5-diazo-1 H-tetrazole Vallee, 1966, 1967), nitration with tetranitromethane (Riordan et uf., 1966), or irradiation with ultraviolet light (Piras and Vallee, 1966). In many instances, altered function has appeared to derive solely from modification of specific side chains of the active center' or substitution of specific metal ions at the active site of the enzyme. However, some reactions have been found to induce changes in protein secondary or tertiary structure and,

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“…Moore and Stein [10] using norleucine as an internal standard [11]. Samples were analyzed on a Durrum D-500 automatic amino acid analyzer after hydrolysis in 6 N HC1 at 110°C for periods of 24, 48, and 72 h. Protein concentrations were determined gravimetrically according to [12], refractometrically with a Beckman analytical ultracentrifuge [13], and from amino acid composition [14]. The copper content was determined with 2,2-biquinoline [15] using an Instrumentation Laboratory Inc. reference standard.…”

Section: North-holland Publishing Company -Amsterdammentioning

confidence: 99%

Neurospora tyrosinase: Molecular weight, copper content and spectral properties

Lerch

1976

FEBS Letters

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Gravimetric Estimation of Proteins Precipitated by Trichloroacetic Acid

Cited by 62 publications

References 20 publications

Isolation and Some Properties of Human Metallothionein^*

Isolation and Some Properties of Human Metallothionein^*

Physicochemical Studies of Carboxypeptidase A Derivatives. II. N-Succinylcarboxypeptidase^*

Neurospora tyrosinase: Molecular weight, copper content and spectral properties

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Gravimetric Estimation of Proteins Precipitated by Trichloroacetic Acid

Cited by 62 publications

References 20 publications

Isolation and Some Properties of Human Metallothionein*

Isolation and Some Properties of Human Metallothionein*

Physicochemical Studies of Carboxypeptidase A Derivatives. II. N-Succinylcarboxypeptidase*

Neurospora tyrosinase: Molecular weight, copper content and spectral properties

Contact Info

Product

Resources

About

Isolation and Some Properties of Human Metallothionein^*

Isolation and Some Properties of Human Metallothionein^*

Physicochemical Studies of Carboxypeptidase A Derivatives. II. N-Succinylcarboxypeptidase^*