Gravimetric Analysis for Determining the Resin and Rubber Content of Guayule

Black, L. M.; Hamerstrand, G. E.; Nakayama, F. S.; Rasnik, B. A.

doi:10.5254/1.3538132

Cited by 59 publications

(24 citation statements)

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“…Duplicate samples were processed to assure uniformity of agreement and to minimize the sampling error . A gravimetric method of rubber and resin analysis (Black et al ., 1983) was employed to spot check the accuracy of the NIR method . Estimates of total rubber and resin yields were calculated by multiplying rubber and resin percentages by the dry weights .…”

Section: Methodsmentioning

confidence: 99%

Relationship of morphological variables to rubber production in guayule

1989

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The objective of this study was to find measurable characters to aid the plant breeder in predicting both rubber percentage and rubber yield of guayule (Parthenium argentatum Gray) . A field of 234 guayule breeding lines was harvested by clipping in February 1986 . Forty-two of these lines were selected for further evaluation on the basis of superior regrowth, rubber content and yield . These 42 lines were reharvested in February 1987 . Nineteen variables were evaluated including percent rubber and resin, rubber and resin yield (g/plant), plant height (cm), width (cm), and volume (m3), fresh and dry weight (kg/plant), percent dry weight, stem number, mean stem diameter (cm), total and mean stem circumference (cm), total and mean stem area (cm 2), total stem circumference/total stem area (cm -1), and regeneration at two dates . Dry weight (kg/plant) had the best correlation to rubber yield (r = 0 .85), and plant volume best predicted dry weight (r = 0 .83) . These variables did not correlate as highly with percent rubber . The best statistical regression model for percent rubber included four variables, fresh and dry weight, percent dry weight, and plant volume . This model accounted for 51% of the variation . In comparison, the best regression of rubber yield had an R2 = 0 .85 for plant height and width, volume, and dry weight .

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Section: Methodsmentioning

confidence: 99%

Relationship of morphological variables to rubber production in guayule

1989

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“…The rubber content of the guayule plants was determined by the procedure of Black et al (1983). The defoliated guayule plants were air dried and cut into small segments with pruning shears and then ground in a Retsch ZM-1 centrifugal mill.…”

Section: Analysis Of Rubber Contentmentioning

confidence: 99%

Seasonal Variations in Rubber Biosynthesis, 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase, and Rubber Transferase Activities in Parthenium argentatum in the Chihuahuan Desert

Benedict

Foster

1993

Plant Physiol.

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“…All plant samples were dried for 2 d at 80°C, weighed, and ground in a coffee grinder, which was wiped clean between samples. Resin and rubber were extracted sequentially from the dried, ground samples with acetone and cyclohexane, respectively, using a common extraction method, which is a variation of the gravimetric analysis method developed by Black et al (1983) A 0.5‐g sample of the ground plant material was homogenized in 20 mL of acetone for 30 s with a Polytron homogenizer (Kinematica, Newark, NJ) at a speed of 27 000 rotations per minute (rpm) to extract resin. The homogenizer was rinsed with an additional 10 mL of acetone and the sample was centrifuged at 3500 rpm for 12 min.…”

Section: Methodsmentioning

confidence: 99%

Water‐Stress‐Induced Changes in Resin and Rubber Concentration and Distribution in Greenhouse‐Grown Guayule

2006

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Guayule (Parthenium argentatum A. Gray) is naturally subject to periods of water stress in its native habitat. It has been shown that, under cultivation, rubber yields increase with increasing irrigation, but rubber concentration per plant decreases. The effect of irrigation on resin concentration is unclear. The purpose of this study was to understand how resin concentration is affected by water stress, and why water stress increases rubber concentration. Greenhouse-grown guayule plants were subjected to water stress in four experiments, each of 3 mo duration. Two experiments were conducted in the summer, the active growth period, and two experiments were conducted in the winter. The water-stressed plants were irrigated when the average soil water potential reached either 20.6 MPa (first summer experiment) or 20.3 MPa (all subsequent experiments). Water-stress effects were monitored by measuring growth, C exchange, biomass, and resin and rubber production. Water-stressed plants had lower C exchange, growth, and leaf-to-stem ratio than well-watered plants. Resin concentration did not respond consistently to water stress. Rubber concentration was generally higher in the water-stressed plants than in the well-watered plants as a result of decreased leaf biomass in both the summer (33 vs. 45 g kg 21 ) and winter (36 vs. 52 g kg 21 ) and decreased stem diameter in the summer (8.1 vs. 11.0 mm). Rubber is deposited mainly in the bark; therefore, reduced leaf biomass and stem diameter contribute to higher rubber concentration in the water-stressed plants by increasing the relative amount of bark.

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Gravimetric Analysis for Determining the Resin and Rubber Content of Guayule

Cited by 59 publications

References 0 publications

Relationship of morphological variables to rubber production in guayule

Relationship of morphological variables to rubber production in guayule

Seasonal Variations in Rubber Biosynthesis, 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase, and Rubber Transferase Activities in Parthenium argentatum in the Chihuahuan Desert

Water‐Stress‐Induced Changes in Resin and Rubber Concentration and Distribution in Greenhouse‐Grown Guayule

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