Grating-coupled surface-plasmon fluorescence DNA sensor

Kasry, Amal; Nicol, Andreas; Knoll, Wolfgang

doi:10.1007/s00340-021-07619-4

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…[ 12,13 ] First, ssDNA probes with a complementary sequence to the target are immobilized on the sensor interface or surface, then, the targeted complementary ssDNA can hybridize with the probe and bind the target for detection. [ 12 ] Different technologies have been developed for tethering DNA probes to substrates. [ 14,15 ] Among them, the patterning into microarrays has become one of the most popular and efficient fabrication approaches for sensing platforms since it evolved in the 1990s, [ 16 ] thus broadly used in DNA hybridization, [ 17 ] protein detection, [ 18 ] and other biosensing systems.…”

Section: Introductionmentioning

confidence: 99%

“…[10,11] Still, basic nucleic acid-sensing strategies are often based on the hybridization of nucleic acids, such as single-stranded DNA (ssDNA) probes that specifically bind their complementary target strand. [12,13] First, ssDNA probes with a complementary sequence to the target are immobilized on the sensor interface or surface, then, the targeted complementary ssDNA can hybridize with the probe and bind the target for detection. [12] Different technologies have been developed for tethering DNA probes to substrates.…”

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confidence: 99%

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Fluorescence Imaging Study of Film Coating Structure and Composition Effects on DNA Hybridization

Yang

Gordiyenko

Schäfer³

et al. 2023

Advanced NanoBiomed Research

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Hybridization of surface‐bound DNA with complementary strands is the basis of many biotechnological applications. Herein, the structure of interfacial coatings between substrate and bound DNA is a crucial element for hybridization behavior. Herein, three reactive surfaces for constructing DNA‐sensing platforms, namely, plain gold films on silicon, poly(bisphenolA‐co‐epichlorohydrin) (PBAG) surfaces with a brush‐like bilayer structure, and dibenzocyclooctyne monolayers (both on glass), are compared. Fluorescence imaging is employed to survey the effect of coating structure and conformation on hybridization performance. To better understand the interfacial structural properties and chemistry of the coated films, atomic force microscopy, water contact angle measurements, and X‐ray photoelectron spectroscopy are employed to characterize the surface morphology. DNA probe microarrays are created on the different platforms via microchannel cantilever spotting, and their performance for hybridizing with the DNA counterparts is assessed. While all three platforms work reliable for DNA detection, a protein‐binding assay reveals that PBAG surfaces offer the highest hybridization efficiency among these approaches. The results of the present work have significant implications for comprehension of the interactions between the DNA hybridization efficiency and the physico‐chemical properties of surface coatings and can inform the fabrication of DNA sensors.

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Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Fluorescence Imaging Study of Film Coating Structure and Composition Effects on DNA Hybridization

Yang

Gordiyenko

Schäfer³

et al. 2023

Advanced NanoBiomed Research

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“…This approach was preferred to aptamers [165] or DNA [166,167] due to closer resemblance to ELISA and draw a clearer parallel with this technique of detection. The assays presented in this chapter are also based on interactions between bovine serum albumin (BSA), human serum albumin (HSA), anti-BSA and anti-HSA.…”

Section: Biosensing 51 Introductionmentioning

confidence: 99%

Development of a new plasmonic transducer for the detection of biological species

Laffont

Crespo‐Monteiro

Berini

et al. 2022

Optical Sensing and Detection VII

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During the COVID-19 outbreak, PCR tests were widely used for large-scale testing and screening.Yet, this technique requires bulky and time-consuming procedures to prepare the samples collected from the patients before their analysis by well-trained experts with expensive and specific equipment. PCR is therefore not competitive as a technique of detection for a widespread and rapid use in point-of-care sites. Thus, the COVID-19 pandemic highlighted the need for cheap and easy-to-implement biosensors. Surface plasmon resonance based sensors were suggested as a promising alternative in recent years. Indeed, they enable real-time and label-free detection of a wide range of analytes. That explains their widespread use in various fields of applications such as pharmacology, toxicology, food safety, and diagnosis. This thesis proposes and demonstrates a new plasmonic configuration of detection, which can address challenges posed by point-of-care settings. The gratings used as transducers in this configuration were fabricated based on laser interference lithography combined with a nanoimprinting process. The responses of these nanostructures interrogated by a p-polarized light beam result in a transfer of energy between two diffracted orders over an angular scan. This optical phenomenon termed as "optical switch", was theoretically and experimentally investigated and optimized.The principle of detection based on this specific configuration was demonstrated for the detection of small variations in the bulk refractive index with solutions comprised of different ratios v Abstract of de-ionized water and glycerol. A limit of detection in the range of 10 −6 RIU was achieved.In addition, preliminary bio-assays obtained by combining this configuration with a functionalization are presented and demonstrate the selectivity and the potential of this new plasmonic configuration for biosensing applications. This thesis work paves the way for the use of the optical switch configuration as a biosensor aligned with low-cost manufacturing and relevant for diagnosing in point-of-care sites. vi List of Figures 1.4 Sketches of the sequential steps for an ELISA assay consisting in detecting and quantifying gentamicin (red Y). A solution with a high gentamicin concentration results in a clearer solution than a solution with a low gentamicin concentration. That manifests through a lower (resp. higher) absorbance for high (resp. low) gentamicin concentrations, as shown in Figure 1.3. These sketches are extracted from [23].

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Quasi-linear response of an integrated optical waveguide sensor by a double output readout method

Zhang

Fan

Cai

et al. 2021

Optik

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Grating-coupled surface-plasmon fluorescence DNA sensor

Cited by 5 publications

References 33 publications

Fluorescence Imaging Study of Film Coating Structure and Composition Effects on DNA Hybridization

Fluorescence Imaging Study of Film Coating Structure and Composition Effects on DNA Hybridization

Development of a new plasmonic transducer for the detection of biological species

Quasi-linear response of an integrated optical waveguide sensor by a double output readout method

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