“…Monitoring the precise variation of temperature is very important for the deep understanding of the reaction mechanism and dynamics in nanosystems, as well as the behavior of a single living cell. − So far, methods developed for determining temperature in nano- and microspaces include thermocouple, heat resistance, thermistor, PN junction and integrated temperature sensor, gallium-filled carbon nanotube sensor, crystal oscillator, and infrared. − The temperature can also be detected by the interaction between temperature and molecular structure with the help of IR spectroscopy, but the in situ and noncontact detection of temperature in nanospace is still a huge challenge in the present technology. As a new emerging member in carbonaceous materials, carbon dots (CDs) have received much attention due to their low cost, good biocompatibility, and unique photoluminescence properties. − Particularly, fluorescent CDs applied in biology-relevant fields have been widely reported, such as cellular imaging, photothermal therapy, and photodynamic therapy. − It is also reported the fluorescence intensity of CDs can decrease with temperature increasing .…”