Graphite-Teflon composite bienzyme electrodes for the determination of l-lactate: Application to food samples

Serra, B.; Reviejo, A.J.; Parrado, Concepción; Pingarrón, José M.

doi:10.1016/s0956-5663(99)00022-6

Cited by 95 publications

(60 citation statements)

References 12 publications

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“…Both batch and flow-injection of a bio-enzyme amperometric composite biosensor were reported to be 1.4 and 0.9 µM L-lactate, respectively. 10 There was no significant loss 1119 ANALYTICAL SCIENCES AUGUST 2004, VOL. 20 of the enzyme activity after six months of storage at 4˚C.…”

Section: ·6 Biological Active Electrodesmentioning

confidence: 99%

“…49,61,65 The use of amperometric biosensors based on horseradish peroxidase provided an alternative process for the measurement of hydrogen peroxide. 10,57,61,71 The design and construction of rigid matrix materials utilized enzyme-graphite-polymer composites. The rigidity of the biocomposite did ensure a simple and lasting immobilization of the enzyme.…”

Section: ·5 Biocomposite Electrodesmentioning

confidence: 99%

“…73 Graphite-Teflon composite bio-enzyme electrodes were demonstrated for the detection of L-lactate in food samples such as wine and yogurt. 10 Lactate oxidase, horseradish peroxidase, and ferrocene (mediator) were incorporated into the graphite-Teflon matrix to develop a bio-enzyme amperometric composite biosensor. Simple physical inclusion of the enzymes into the bulk of a graphite-Teflon pellet with no covalent attachment was utilized to prepare a graphite-Teflon biocomposite whose surface was easily regenerated by polishing.…”

Section: ·6 Biological Active Electrodesmentioning

confidence: 99%

“…Two broad categories of biosensors based on the biological active substances are catalytic biosensors like whole cell electrodes and affinity biosensors such as nucleic acid electrodes. 6,10 In addition, biosensors could be classified into direct (label-free) and indirect (labeled) detectors of target analyte. For instance, a preliminary biochemical reaction takes place and the products of the reaction are detected by the indirect detection of electrochemical biosensors.…”

Section: Introductionmentioning

confidence: 99%

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Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

2004

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The objective of this study is to analyze the technical importance, performance, techniques, advantages, and disadvantages of the biosensors in general and of the electrochemical biosensors in particular. A product of reaction diffuses to the transducer in the first generation biosensors (based on Clark biosensors). The mediated biosensors or second generation biosensors use specific mediators between the reaction and the transducer to improve sensitivity. The second generation biosensors involve two steps: first, there is a redox reaction between enzyme and substrate that is reoxidized by the mediator, and eventually the mediator is oxidized by the electrode. No normal product or mediator diffusion is directly involved in the third generation biosensors, direct biosensors. Based on the type of transducer, current biosensors are divided into optical, mass, thermal, and electrochemical sensors. They are used in medical diagnostics, food quality controls, environmental monitoring, and other applications. These biosensors are also grouped under two broad categories of sensors: direct and indirect detection systems. Moreover, these systems could be further grouped into continuous or batch operation. Therefore, amperometric biosensors and their current applications are focused on more in detail since they are the most commonly used biosensors in monitoring and diagnosing tests in clinical analysis. Problems related to the commercialization of medical, environmental, and industrial biosensors as well as their performance characteristics, their competitiveness in comparison to the conventional analytical tools, and their costs determine the future development of these biosensors.

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Section: ·6 Biological Active Electrodesmentioning

confidence: 99%

Section: ·5 Biocomposite Electrodesmentioning

confidence: 99%

Section: ·6 Biological Active Electrodesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

2004

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“…Analysis of wines and other beverages attracts a particular attention since it requires only a minor sample preparation procedure. A number of chromatographic, spectrometric and electrochemical detection systems were designed for carbohydrates [10], l-lactate [11,12], l-malate [13], glycerol [14], alcohols [15], phenolic [16], sulfuric [17] and aromatic [18,19] compounds. Spectrometric and chromatographic methods of analysis including mass-spectrometry, HPLC, micro-extraction and electrophoresis in some cases are more sensitive than electrochemical ones but much more laborious and expensive.…”

Section: Introductionmentioning

confidence: 99%

Flow‐Injection Analysis of Residual Glucose in Wines Using a Semiautomatic Analyzer Equipped with a Prussian Blue‐Based Biosensor

Ulasova¹,

Micheli²,

Vasii³

et al. 2003

Electroanalysis

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The Prussian Blue modified glassy-carbon (GC) electrode assembled with glucose oxidase (GOD) immobilized in Nafion on the top of the electrode surface area has been inserted in a wall-jet cell connected to a semiautomatic analyzer, which controls all the operations for carrier buffer, flow and sample injection. The Prussian Blue based glucose biosensor inserted in the FIA analyzer has shown linear response to glucose at an applied potential of 0.0 V (Ag/AgCl) in the range of 10 À6 ± 10 À3 M. Residual glucose has been detected in red and white wines. A dilution of 1 : 1000 has been used for all the wines tested. Neither matrix effect, nor electrochemical interferences were observed. A comparison with a spectrophotometric glucose kit gave excellent correlation for levels of glucose detected. FIA analyzer compared with the standard analytical kit resulted in simplified procedures and a reduced time for the analysis.

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Biotechnology

Becker¹,

Breithaupt²,

Doelle³

et al. 2007

Ullmann's Encyclopedia of Industrial Chemistry

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Graphite-Teflon composite bienzyme electrodes for the determination of l-lactate: Application to food samples

Cited by 95 publications

References 12 publications

Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

Flow‐Injection Analysis of Residual Glucose in Wines Using a Semiautomatic Analyzer Equipped with a Prussian Blue‐Based Biosensor

Biotechnology

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