Graphical Data Representation Methods To Assess the Quality of LC Columns

Desmet, Gert; Cabooter, Deirdre; Broeckhoven, Ken

doi:10.1021/ac504473p

Cited by 39 publications

(24 citation statements)

References 76 publications

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“…The easiest and commonly used way to evaluate the column efficiency in isocratic separation is done by van Deemter analysis that is illustrated in Eq. .

H = A + \frac{B}{u_{0}} + C \cdot u_{0}

where A is the eddy diffusion, B the longitudinal diffusion, C the mass transfer, and u 0 the linear velocity.…”

Section: Resultsmentioning

confidence: 99%

“…

t_{0} = \frac{Δ P_{\max}}{η} \cdot {([], \frac{K_{v 0}}{u_{0}^{2}})}_{\exp}

N = \frac{Δ P_{trueprefixmax}}{η} \cdot {([], \frac{K_{v 0}}{u_{0} \cdot H})}_{\exp}

K_{v 0} = \frac{u_{0} \cdot η \cdot L}{((), Δ P_{t o t a l} - Δ P_{s y s t e m})}

where η is the mobile phase viscosity. This curve illustrates the maximum achievable efficiency that can be reached for each stationary phase material in a given time at ∆P max . The comparison of the KPL curves allows for conclusions about the most efficient column and gives information about the corresponding column length.…”

Section: Resultsmentioning

confidence: 99%

“…The easiest and commonly used way to evaluate the column efficiency in isocratic separation is done by van Deemter analysis that is illustrated in Eq. (2) [15,23].…”

Section: Determination Of Efficiency By Van Deemter Analysismentioning

confidence: 99%

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Characterization of the efficiency of microbore liquid chromatography columns by van Deemter and kinetic plot analysis

et al. 2016

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The efficiency of miniaturized liquid chromatography columns with inner diameters between 200 and 300 μm has been investigated using a dedicated micro-liquid chromatography system. Fully porous, core-shell and monolithic commercially available stationary phases were compared applying van Deemter and kinetic plot analysis. The sub-2 μm fully porous as well as the 2.7 μm core-shell particle packed columns showed superior efficiency and similar values for the minimum reduced plate heights (2.56-2.69) before correction for extra-column contribution compared to normal-bore columns. Moreover, the influence of extra-column contribution was investigated to demonstrate the difference between apparent and intrinsic efficiency by replacing the column by a zero dead volume union to determine the band spreading caused by the system. It was demonstrated that 72% of the intrinsic efficiency could be reached. The results of the kinetic plot analysis indicate the superior performance of the sub-2 μm fully porous particle packed column for ultra-fast liquid chromatography.

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“…The easiest and commonly used way to evaluate the column efficiency in isocratic separation is done by van Deemter analysis that is illustrated in Eq. .

H = A + \frac{B}{u_{0}} + C \cdot u_{0}

where A is the eddy diffusion, B the longitudinal diffusion, C the mass transfer, and u 0 the linear velocity.…”

Section: Resultsmentioning

confidence: 99%

“…

t_{0} = \frac{Δ P_{\max}}{η} \cdot {([], \frac{K_{v 0}}{u_{0}^{2}})}_{\exp}

N = \frac{Δ P_{trueprefixmax}}{η} \cdot {([], \frac{K_{v 0}}{u_{0} \cdot H})}_{\exp}

K_{v 0} = \frac{u_{0} \cdot η \cdot L}{((), Δ P_{t o t a l} - Δ P_{s y s t e m})}

Section: Resultsmentioning

confidence: 99%

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Characterization of the efficiency of microbore liquid chromatography columns by van Deemter and kinetic plot analysis

et al. 2016

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“…Even more numerous are the types of graphical presentations of data evaluated with the help of these equations. In a very recent publication [81] Desmet et al collected all possible presentations of kinetic plots and separated them into two groups: one group for column users and another group for theoreticians and column producers. The difference between the kinetic plots in two groups is only the parameters to be optimized and the parameters to be varied.…”

Section: Resultsmentioning

confidence: 99%

Application of kinetic plots in gas and liquid chromatography for the optimization of separation conditions

Kurganov

Kanat’eva

Yakubenko

2015

J of Separation Science

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This review describes the recent achievements and historical background of investigations in optimization procedures in gas and liquid chromatographic techniques. The optimization parameters are varied depending on researcher interests and may include separation efficiency, analysis time, column operation pressure, packing characteristics, etc. As it is shown the kinetic performance limit is one of the most used and vital concepts for gas and liquid chromatography. Application of the concept sometimes requires additional cumbersome calculations but nevertheless it provides the most grounded comparison of different packings, columns, and techniques.

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“…KV is a physical constant that depends on the diameter of the packing particles and the specific surface area, and is inversely proportional to the flow resistance against ΔP. The methods give us a kinetic performance limit (KPL) 32 of t0 and N, incorporating the length of the column (L), uniquely calculated with u0 as an operating parameter. L is the other operating parameter, and the largest limit to be automatically obtained at u0 with the specified ΔP.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional Representation Method Using Pressure, Time, and Number of Theoretical Plates to Analyze Separation Conditions in HPLC Columns

2018

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There has been considerable discussion of the speed performance of HPLC separation, especially regarding the relationship between theoretical plates and hold-up time. The fundamental discussion focuses on the optimal velocity, u0,opt, which gives a minimal height equivalent to a theoretical plate of the van Deemter plot. On the other hand, Desmet's method, using the kinetic performance limit (KPL), calculates the highest performance with a constant pressure drop, without focusing solely on the optimal velocity. In this paper, a precise method based on the KPL is proposed, to understand how increasing pressure enhances both theoretical plates and hold-up time. A three-dimensional representation method that combines the pressure drop with two axes of time and theoretical plates will be useful for discussing the effect of pressure in pressure-driven chromatography. Using three dimensions, the methods based on u0,opt and the KPL can be combined, because u0,opt can be visualized three-dimensionally, including the neighbor of u0,opt; and the question of whether the KPL is an asymptotic or effective limit can be investigated. Three performances of high resolution, high speed, and low pressure can be understood on different packing supports at a glance.

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Graphical Data Representation Methods To Assess the Quality of LC Columns

Cited by 39 publications

References 76 publications

Characterization of the efficiency of microbore liquid chromatography columns by van Deemter and kinetic plot analysis

Characterization of the efficiency of microbore liquid chromatography columns by van Deemter and kinetic plot analysis

Application of kinetic plots in gas and liquid chromatography for the optimization of separation conditions

Three-dimensional Representation Method Using Pressure, Time, and Number of Theoretical Plates to Analyze Separation Conditions in HPLC Columns

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