Graphene substrate for inducing neurite outgrowth

Lee, Jeong Soon; Lipatov, Alexey; Ha, Ligyeom; Shekhirev, Mikhail; Andalib, Mohammad Nahid; Sinitskii, Alexander; Lim, Jung Yul

doi:10.1016/j.bbrc.2015.03.023

Cited by 53 publications

(48 citation statements)

References 28 publications

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“…However, in these previous studies cells were plated on those materials previously coated with extracellular matrix proteins, which are known to promote cell adhesion, differentiation, and neurite outgrowth (Vicario et al, 1993; Calof et al, 1994; Specht et al, 2004; Otaegi et al, 2006; Nishimune et al, 2008). To the best of our knowledge, no studies reporting the biocompatibility of uncoated graphene with aNSCs and aNSC-derived neurons and glia have yet been published (Bendali et al, 2013; Sahni et al, 2013; Lee et al, 2015; Fabbro et al, 2016; Veliev et al, 2016). Here, we have studied the biocompatibility of uncoated TRG with aOBSCs and aOBSC-derived with neurons, astrocytes, and oligodendrocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, very few works have addressed the effect of uncoated graphene on the growth of neurons and glial cells. They reported that neurons can develop on graphene but their attachment was reduced compared to when the neurons were grown on poly- d -Lysine and laminin (Bendali et al, 2013; Sahni et al, 2013), that graphene stimulated neurite length compared to a glass substrate (Lee et al, 2015), or that pristine graphene and graphene-based substrates were permissive for neuronal outgrowth (Veliev et al, 2016) and synapse formation and function (Fabbro et al, 2016). …”

Section: Introductionmentioning

confidence: 99%

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In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia

Defteralı

Verdejo

Majeed

et al. 2016

Front. Bioeng. Biotechnol.

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Graphene, graphene-based nanomaterials (GBNs), and carbon nanotubes (CNTs) are being investigated as potential substrates for the growth of neural cells. However, in most in vitro studies, the cells were seeded on these materials coated with various proteins implying that the observed effects on the cells could not solely be attributed to the GBN and CNT properties. Here, we studied the biocompatibility of uncoated thermally reduced graphene (TRG) and poly(vinylidene fluoride) (PVDF) membranes loaded with multi-walled CNTs (MWCNTs) using neural stem cells isolated from the adult mouse olfactory bulb (termed aOBSCs). When aOBSCs were induced to differentiate on coverslips treated with TRG or control materials (polyethyleneimine-PEI and polyornithine plus fibronectin-PLO/F) in a serum-free medium, neurons, astrocytes, and oligodendrocytes were generated in all conditions, indicating that TRG permits the multi-lineage differentiation of aOBSCs. However, the total number of cells was reduced on both PEI and TRG. In a serum-containing medium, aOBSC-derived neurons and oligodendrocytes grown on TRG were more numerous than in controls; the neurons developed synaptic boutons and oligodendrocytes were more branched. In contrast, neurons growing on PVDF membranes had reduced neurite branching, and on MWCNTs-loaded membranes oligodendrocytes were lower in numbers than in controls. Overall, these findings indicate that uncoated TRG may be biocompatible with the generation, differentiation, and maturation of aOBSC-derived neurons and glial cells, implying a potential use for TRG to study functional neuronal networks.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia

Defteralı

Verdejo

Majeed

et al. 2016

Front. Bioeng. Biotechnol.

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“…However, the conductivity of PPy did not meet the need for the in vivo ES, due to the worse hydrophilicity and short‐time conductivity (Guimard et al, ). Recently, graphene, as a promising candidate in biomedical applications, could be applied in nerve repair due to the sustained electroactive (Golafshan, Kharaziha, & Fathi, ; Park et al, ), and graphene materials could also promote neural stem cells differentiation, and facilitate neurite sprouting and outgrowth (Akhavan & Ghaderi, ; Akhavan, Ghaderi, Abouei, Hatamie, & Ghasemi, ; J. S. Lee et al, ). Kurapati et al found that graphene materials could be degraded via the in vivo effect of activated neutrophils into the smaller size nanosheets for the elimination from the body (Kurapati et al, ), suggesting the in vivo potential application of graphene.…”

Section: Introductionmentioning

confidence: 99%

“…However, the conductivity of PPy did not meet the need for the in vivo ES, due to the worse hydrophilicity and short-time conductivity (Guimard et al, 2007). Recently, graphene, as a promising candidate in biomedical applications, could be applied in nerve repair due to the sustained electroactive (Golafshan, Kharaziha, & Fathi, 2017;Park et al, 2011), and graphene materials could also promote neural stem cells differentiation, and facilitate neurite sprouting and outgrowth Akhavan, Ghaderi, Abouei, Hatamie, & Ghasemi, 2014;J. S. Lee et al, 2015).…”

mentioning

confidence: 99%

Preparation of carboxylic graphene oxide‐composited polypyrrole conduits and their effect on sciatic nerve repair under electrical stimulation

Chen

Liu

Huang

et al. 2019

J Biomedical Materials Res

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Carboxylic graphene oxide‐composited polypyrrole/poly‐l‐lactic acid (C‐GO/PPy/PLLA) films were fabricated by electrochemical deposition of C‐GO‐composited PPy on PLLA fibers‐film, and their conductivity and tensile strength (∼4.6 S/cm and 26.4 MPa, respectively) were stably remained after the immersion of 4 weeks, due to the hydrogen bond interaction between graphene oxide's carboxylic groups and pyrrole's imino groups. Their specific surface areas of ∼57.5 m2/g and pore volume of ∼0.02 cm3/g were significantly larger than those of PPy/PLLA films, due to the addition of C‐GO nanosheets. Then, C‐GO/PPy/PLLA conducting conduit with 2 mm inner diameter was prepared to bridge 10 mm sciatic nerve defect of rats, and the direction of fiber‐axis in the conduit was the same as the conduit central axis. Electrical stimulation (ES) of 1 V and 20 Hz through the conducting conduit was exerted on the defect site. The results of in vivo electrophysiological and histological evaluation indicated that, the sciatic nerve defect could be repaired in C‐GO/PPy/PLLA conduit, moreover the re‐innervated gastrocnemius muscle and nerve conduction in C‐GO/PPy/PLLA conduit & ES group were obviously better than the conduit without ES group. The results of transmission electron microscope analysis also demonstrated that the mean thickness of myelin sheath and diameter of axon in C‐GO/PPy/PLLA conduit & ES group were significantly larger than those without ES, suggesting that the repair efficiency of ES & conduit group was closer to that of autograft group. These results indicated the great potential of C‐GO/PPy/PLLA with the in vivo ES in the application of sciatic nerve repair.

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“…The resultant studies suggest that neurons can be grown on bare graphene adhered to glass, but are conflicted on whether graphene influences cell viability and vitality. [10,23,25,26,28] Studies that combine graphene and matrix substrates utilize platforms in which graphene is sandwiched between a thin glass slide and a layer of matrix substrate, with cells plated on top of the matrix substrate. While this approach does achieve a combinatorial substrate of graphene and organic matrix, direct contact between cellular processes and graphene is prevented.…”

Section: Introductionmentioning

confidence: 99%

Impact of Graphene on the Efficacy of Neuron Culture Substrates

Fischer

Zhang

Risner

et al. 2018

Adv Healthcare Materials

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How graphene influences the behavior of living cells or tissues remains a critical issue for its application in biomedical studies, despite the general acceptance that graphene is biocompatible. While direct contact between cells and graphene is not a requirement for all biomedical applications, it is often mandatory for biosensing. Therefore, it is important to clarify whether graphene impedes the ability of cells to interact with biological elements in their environment. Here, a systematic study is reported to determine whether applying graphene on top of matrix substrates masks interactions between these substrates and retinal ganglion cells (RGCs). Six different platforms are tested for primary RGC cultures with three platforms comprised of matrix substrates compatible with these neurons, and another three having a layer of graphene placed on top of the matrix substrates. The results demonstrate that graphene does not impede interactions between RGCs and underlying substrate matrix, such that their positive or negative effects on neuron viability and vitality are retained. However, direct contact between RGCs and graphene reduces the number, but increases basal activity, of functional cation channels. The data indicate that, when proper baselines are established, graphene is a promising biosensing material for in vitro applications in neuroscience.

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Graphene substrate for inducing neurite outgrowth

Cited by 53 publications

References 28 publications

In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia

In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia

Preparation of carboxylic graphene oxide‐composited polypyrrole conduits and their effect on sciatic nerve repair under electrical stimulation

Impact of Graphene on the Efficacy of Neuron Culture Substrates

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