Graphene quantum dots suppress proinflammatory T cell responses via autophagy-dependent induction of tolerogenic dendritic cells

Tomić, Sergej; Janjetović, Kristina; Mihajlović, Dušan; Milenković, Marina; Kravić-Stevović, Tamara; Marković, Zoran M.; Marković, Biljana M. Todorović; Špitálský, Zdenko; Mičušík, Matej; Vučević, Dragana; Čolić, Miodrag; Trajkovič, Vladimir

doi:10.1016/j.biomaterials.2017.08.040

Cited by 85 publications

(82 citation statements)

References 77 publications

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“…PBMC was isolated from three healthy volunteers as described previously . Cells were seeded in a 96 well plate at 2 × 10 5 cells/well and stimulated with ES L1 (50 µg/mL) or with of T spiralis EVs (2.5, 1 and 0.25 µg/mL).…”

Section: Methodsmentioning

confidence: 99%

“…PBMC was isolated from three healthy volunteers as described previously. 19 Cells were seeded in a 96 well plate at 2 × 10 5 cells/well and stimulated with ES L1 (50 µg/mL) or with of T spiralis EVs (2.5, 1 and 0.25 µg/mL). After 3 days of cultivation, production of cytokines was determined in cell-conditioned media using the LEGENDPlex, Human Th Cytokine Panel (13- plex: IL10, IL9, IL6, IL2, IL13, IL5, IL22, IL21, IL4, IL17f, IL17a, TNFα, INFγ) (CatNo.…”

Section: Pbmc Stimulation Assaymentioning

confidence: 99%

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Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties

Kosanović

Cvetković

Gruden-Movsesijan

et al. 2019

Parasite Immunology

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Aims Extracellular vesicles (EVs) represent a newly discovered but universal communication tool between cells or organisms. However, few data exist on nematode EVs and none for Trichinella spiralis. Here, we aimed to investigate whether T spiralis muscle larvae produce EVs, whether they carry immunomodulatory proteins and whether they have a role in immunomodulation as a component of excretory‐secretory muscle larvae products (ES L1). Methods and results EVs were enriched from conditioned medium of T spiralis muscle larvae. Transmission electron microscopy images showed T spiralis EVs to be 30‐80 nm in size, and Western blot confirmed the presence of two out of three glycoproteins with the immunodominant epitope characteristic for muscle larvae of the genus Trichinella. Using a peripheral blood mononuclear cell (PBMC) stimulation assay, it was shown that these EVs elevated production of IL10 and IL6. Conclusion T spiralis muscle larvae produce EVs. Those EVs carry immunomodulatory proteins and have the capacity independently to induce regulatory responses in the same way as the T spiralis excretory‐secretory muscle larvae products from which they were isolated.

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Section: Methodsmentioning

confidence: 99%

Section: Pbmc Stimulation Assaymentioning

confidence: 99%

Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties

Kosanović

Cvetković

Gruden-Movsesijan

et al. 2019

Parasite Immunology

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“…Graphene quantum dots are anti‐inflammatory. Graphene quantum dots have been shown to cause dendritic cells to increase secretion of IL‐27 as well as decrease secretion of IL‐12 and IL‐23 . Further, graphene quantum dots have been shown to suppress the ability of dendritic cells to produce Th1 and Th17 cells and have helped increase the production of Th2 cells with regulatory T cell phenotypes.…”

Section: Immunomodulatory Materialsmentioning

confidence: 99%

Materials for Immunotherapy

et al. 2019

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Breakthroughs in materials engineering have accelerated the progress of immunotherapy in preclinical studies. The interplay of chemistry and materials has resulted in improved loading, targeting, and release of immunomodulatory agents. An overview of the materials that are used to enable or improve the success of immunotherapies in preclinical studies is presented, from immunosuppressive to proinflammatory strategies, with particular emphasis on technologies poised for clinical translation. The materials are organized based on their characteristic length scale, whereby the enabling feature of each technology is organized by the structure of that material. For example, the mechanisms by which i) nanoscale materials can improve targeting and infiltration of immunomodulatory payloads into tissues and cells, ii) microscale materials can facilitate cell‐mediated transport and serve as artificial antigen‐presenting cells, and iii) macroscale materials can form the basis of artificial microenvironments to promote cell infiltration and reprogramming are discussed. As a step toward establishing a set of design rules for future immunotherapies, materials that intrinsically activate or suppress the immune system are reviewed. Finally, a brief outlook on the trajectory of these systems and how they may be improved to address unsolved challenges in cancer, infectious diseases, and autoimmunity is presented.

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“…As described in Section , recent studies have demonstrated that biomaterials display intrinsic properties that can drive inflammatory or proimmune function in immune cells. Other recent studies have described tolerogenic properties of various biomaterials, including synthetic polymers such as PLGA and PS, QDs, gold NPs, and cellulose nanofibers . These intrinsic biomaterial properties are now starting to be explored in therapeutic contexts as tools to promote tolerance in preclinical models of autoimmune disease.…”

Section: Biomaterials Display Intrinsic Immunogenic Materials Propertimentioning

confidence: 99%

“…Other studies have investigated QDs to drive autophagy as well . These studies demonstrated that graphene QDs reduced secretion of inflammatory cytokines, production of ROS and expression of CD40, CD80, and CD86 in DCs.…”

Section: Biomaterials Display Intrinsic Immunogenic Materials Propertimentioning

confidence: 99%

Engineering Immune Tolerance with Biomaterials

Gammon

Jewell

2019

Adv Healthcare Materials

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Autoimmune diseases, rejection of transplanted organs and grafts, chronic inflammatory diseases, and immune‐mediated rejection of biologic drugs impact a large number of people across the globe. New understanding of immune function is revealing exciting opportunities to help tackle these challenges by harnessing—or correcting—the specificity of immune function. However, realizing this potential requires precision control over the interaction between regulatory immune cues, antigens attacked during inflammation, and the tissues where these processes occur. Engineered materials—such as polymeric and lipid particles, scaffolds, and inorganic materials—offer powerful features that can help to selectively regulate immune function during disease without compromising healthy immune functions. This review highlights some of the exciting developments to leverage biomaterials as carriers, depots, scaffolds—and even as agents with intrinsic immunomodulatory features—to promote immunological tolerance.

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Graphene quantum dots suppress proinflammatory T cell responses via autophagy-dependent induction of tolerogenic dendritic cells

Cited by 85 publications

References 77 publications

Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties

Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties

Materials for Immunotherapy

Engineering Immune Tolerance with Biomaterials

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