Graphene Quantum Dots Protect against Copper Redox-Mediated Free Radical Generation and Cardiac Cell Injury

Li, Y. Robert; Santo, Arben; Zhu, Hong; Jia, Zhenquan; Trush, Michael A.

doi:10.20455/ros.2018.855

Cited by 2 publications

(4 citation statements)

References 17 publications

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“…We recently showed that GQDs could potently block the Cu(II)/Cu(I) redox cycle likely via sequestering the copper ions, thereby preventing them from participating in the redox reactions [6]. Accordingly, inhibition of the Cu(II)/Cu(I) redox cycle may be primarily responsible for the protective effects of GQDs on oxidative DNA damage.…”

Section: Resultsmentioning

confidence: 99%

“…The EPR sample reactions were carried out in 0.1 ml DpBS, and the samples were loaded into 50-μl capillary tubes (Drummond Scientific, Broomall, PA, USA) before subjecting to the ERP measurement [6]. …”

Section: Methodsmentioning

confidence: 99%

“…Over the past several years, graphene quantum dots (GQDs) have been emerging as a nanotech modality with tremendous applications in biomedicine, especially in bioimaging and cancer drug delivery [5]. Recently, we have demonstrated that GQDs may block copper redox-mediated free radical generation and cardiac cell injury [6]. Hence, we proposed that GQDs may also block copper redox-mediated oxidative DNA damage.…”

Section: Introductionmentioning

confidence: 99%

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Graphene Quantum Dots Potently Block Copper-Mediated Oxidative DNA Damage: Implications for Cancer Intervention

Li¹,

Zhu

et al. 2018

ROS

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Our early work suggested that graphene quantum dots (GQDs) block Cu(II)/Cu(I) redox cycle in biological systems. Here we report that GQDs could also potently protect against copper redox-mediated oxidative DNA damage. Using Cu(II)/hydrogen peroxide, Cu(II)/hydroquinone, and Cu(II)/ascorbic acid as three biologically relevant systems for inducing oxidative DNA damage, we demonstrated that GQDs protected against the above system-induced DNA strand breaks in ϕx-174 plasmid DNA in a concentration-dependent manner. Notably, a significant protection was observed with GQDs at 1 μg/ml, and a nearly complete protection was shown with 10 and 100 μg/ml of GQDs. Using electron paramagnetic resonance (EPR) spectrometry in conjunction with α-(4-pyridyl-1-oxide)-N-tert-butylnitrone (POBN)-spin trapping, we showed that the above three systems generated hydroxyl radicals, as evidenced by the formation of a POBN-CH3 radical adduct in the presence of 0.5 M dimethyl sulfoxide (DMSO). Consistent with the protective effects of GQDs on DNA damage, the hydroxyl radical formation was markedly reduced in the presence of GQDs in a concentration dependent manner. A nearly complete blockage of the hydroxyl radical generation was seen with GQDs at 10 and 100 μg/ml. Taken together, our results showed that GQDs potently protected against oxidative DNA damage. Considering the critical role of copper in cancer development, our findings might have important implications for cancer intervention with GQD-based nanotech modality.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Graphene Quantum Dots Potently Block Copper-Mediated Oxidative DNA Damage: Implications for Cancer Intervention

Li¹,

Zhu

et al. 2018

ROS

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“…In a study, GQDs were tested for their ability to scavenge free radicals in vitro in cardiomyocytes. It was found that GQDs at a concentration of 1 μg/ml were able to protect cardiomyocytes from H 2 O 2 -mediated cell injury [ 185 ].…”

Section: Application Of Cnms In Cardiovascular Tissue Engineeringmentioning

confidence: 99%

Carbon nanomaterials for cardiovascular theranostics: Promises and challenges

Alagarsamy

Mathan

Yan

et al. 2021

Bioactive Materials

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Cardiovascular diseases (CVDs) are the leading cause of death worldwide. Heart attack and stroke cause irreversible tissue damage. The currently available treatment options are limited to “damage-control” rather than tissue repair. The recent advances in nanomaterials have offered novel approaches to restore tissue function after injury. In particular, carbon nanomaterials (CNMs) have shown significant promise to bridge the gap in clinical translation of biomaterial based therapies. This family of carbon allotropes (including graphenes, carbon nanotubes and fullerenes) have unique physiochemical properties, including exceptional mechanical strength, electrical conductivity, chemical behaviour, thermal stability and optical properties. These intrinsic properties make CNMs ideal materials for use in cardiovascular theranostics. This review is focused on recent efforts in the diagnosis and treatment of heart diseases using graphenes and carbon nanotubes. The first section introduces currently available derivatives of graphenes and carbon nanotubes and discusses some of the key characteristics of these materials. The second section covers their application in drug delivery, biosensors, tissue engineering and immunomodulation with a focus on cardiovascular applications. The final section discusses current shortcomings and limitations of CNMs in cardiovascular applications and reviews ongoing efforts to address these concerns and to bring CNMs from bench to bedside.

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Graphene Quantum Dots Protect against Copper Redox-Mediated Free Radical Generation and Cardiac Cell Injury

Cited by 2 publications

References 17 publications

Graphene Quantum Dots Potently Block Copper-Mediated Oxidative DNA Damage: Implications for Cancer Intervention

Graphene Quantum Dots Potently Block Copper-Mediated Oxidative DNA Damage: Implications for Cancer Intervention

Carbon nanomaterials for cardiovascular theranostics: Promises and challenges

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