Graphene quantum dots enhance anticancer activity of cisplatin via increasing its cellular and nuclear uptake

Sui, Xin; Luo, Chao; Wang, Chong; Zhang, Fangwei; Zhang, Jingyan; Guo, Shouwu

doi:10.1016/j.nano.2016.03.010

Cited by 83 publications

(63 citation statements)

References 43 publications

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“…27 Chemotherapeutic drugs/GQD-based nanosystem is developed to enhance efficiency of drug to kill cancerous cells. 28 It is observed that this nanosystem can increase the chemotherapeutic efficacy of anticancer drugs. 29 Until now, most of the studies related to the development of theranostic tool for cancer using GQDs was based on the use of standard chemotherapeutic drugs.…”

Section: Introductionmentioning

confidence: 99%

<p>Development of biosurfactant-based graphene quantum dot conjugate as a novel and fluorescent theranostic tool for cancer</p>

Bansal

Singh

Kumari

et al. 2019

IJN

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BackgroundBiosurfactants are amphipathic molecules of microbial origin that reduce surface and interfacial tension at gas–liquid–solid interfaces. Earlier, the biosurfactant was isolated and characterized in our laboratory from Candida parapsilosis. The property of the biosurfactant is further explored in this study by using quantum dots (QDs) as nanocarrier.Materials and methodsGraphene quantum dots (GQDs) were synthesized by bottom-up approach through pyrolysis of citric acid. GQDs were conjugated with both biosurfactant and folic acid (FA) using carbodiimide chemistry. The prepared GQD bioconjugate was studied for diagnostic and therapeutic effects against cancer cells.Results and discussionPhotoluminescence quantum yield (QY) of plain GQDs was measured as 12.8%. QY for biosurfactant conjugated GQDs and FA-biosurfactant conjugated GQDs was measured as 10.4% and 9.02%, respectively, and it was sufficient for targeting cancer cells. MTT assay showed that more than 90% of cells remained viable at concentration of 1 mg/mL, hence GQDs seemed to be non-toxic to cells. Biosurfactant conjugated GQDs caused 50% reduction in cellular viability within 24 hours. FA conjugation further increased the specificity of bioconjugated GQDs toward tumor cells, which is clearly evident from the drug internalization studies using confocal laser scanning microscopy. A higher amount of drug uptake was observed when bioconjugated GQDs were decorated with FA.ConclusionThe ability of GQD bioconjugate could be used as a theranostic tool for cancer. It is foreseen that in near future cancer can be detected and/or treated at an early stage by utilizing biosurfactant conjugated GQDs. Therefore, the proposed study would provide a stepping stone to improve the life of cancer patients.

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Section: Introductionmentioning

confidence: 99%

<p>Development of biosurfactant-based graphene quantum dot conjugate as a novel and fluorescent theranostic tool for cancer</p>

Bansal

Singh

Kumari

et al. 2019

IJN

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“…The medium was changed every two days during the incubation. Cells were made quiescent by starvation after growth to 70-80% confluence and then treated with experimental reagent [20].…”

Section: Eanticancer Activities Of the Synthesized Cse-gqdsmentioning

confidence: 99%

“…The % cell viability was calculated using the following formula: % Cell viability = A570 of treated cells / A570 of control cells × 100 Graphs are plotted using the % of Cell Viability at Y-axis and concentration of the sample in X-axis. Cell control and sample control is included in each assay to compare the full cell viability assessments [20].…”

Section: Fcell Viability Assaymentioning

confidence: 99%

Cocoa Bean-Extract Mediated Graphene Quantum Dots as Antimicrobial, Anticancer and Plant Growth Regulators

2019

IJRTE

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A facile method has been developed for the first time for the synthesis of graphene quantum dots using The obroma cacao (cocoa seeds) by hydrothermal method. The structure and morphology of ensuring product was characterized by UV, FTIR, X-ray Diffraction (PXRD) and TEM and SAED pattern. Then anti-microbial, anti-oxidant and anti-cancer activities of T. cacao mediated graphene quantum dots were analyzed. Further investigations were made on the use of T. cacao mediated graphene quantum dots for growth enhancement in green gram (Vigna radiate). CSE-GQDs (Cocoa seed extract-Graphene quantum dots) showed greatest antimicrobial activity against the Staphylococcus aureus, Pseudomonas aeroginosa, Streptococcus mutans, Salmonella sp, Escherichia coli, Microsporum gypseum, Trichophyton sp. The results of antioxidant activity of the CSE- GQDs evaluated using DPPH scavenging assays indicates that the CSE- GQDs possess a higher scavenging activity. The cytotoxicity studies showed that the synthesized CSE- GQDs exhibited a dose dependent toxicity on human breast cancer (MCF-7) cell lines. The IC50 value for MCF-7 cells is found to be 31.2µg/ml of CSE- GQDs, where a 50% noticed cell death is observed. The germination rate of green gram seeds significantly increased after treatment with higher CSE-GQDs. Our investigations open up the opportunity to use graphene quantum dots as plant growth regulators that can be used in a variety of other food plants for high yield.

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“…Reports of numerous groups working on the improvement of Cis therapy have already shown that several compounds possess these unique properties. In particular, Sui et al demonstrated that yet another nanocarbon structure -graphene -also enhances Cis anticancer activity by increasing its uptake to the cell and its nucleus [53], the mechanism also described for FC 60 [14][15][16]. Structurally different nanocarriers, such as shikonin [52] or dibenzo[c,h][1,6]naphthyridin-6-one [54] also increased Cis cytotoxicity towards HCT-116 cell line, while Cis complex with benzyl isothiocyanate [55] demonstrated similar effects in the HL-60 cell line.…”

Section: 3mentioning

confidence: 99%

C60 fullerene enhances cisplatin anticancer activity and overcomes tumor cell drug resistance

et al. 2016

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A novel nano-formulation of the anticancer drug cisplatin (Cis) with C 60 fullerene (C 60 +Cis complex) was developed, demonstrating enhanced cytotoxic activity towards tumor cell lines in vitro n comparison to Cis alone. The enhanced proapoptotic activity of the novel complexes was found to be tightly connected with their unique capability to circumvent cancer drug resistance in vitro, as revealed by investigation of human leukemia cells HL-60 together with their sublines resistant towards doxorubicin (HL-60/adr, multidrug resistance protein-1=MRP-1=ABCC1 overexpressing) and vincristine (HL-60/vinc, P-glycoprotein=P-gp=ABCB1 overexpressing). The enhanced anticancer activity of the developed С 60 +Cis complexes was also confirmed in vivo on male C57BL/6J mice bearing Lewis lung carcinoma, effectively inhibiting tumor growth and formation of metastases in comparison to free single Cis. For better understanding of molecular mechanisms underlying the potential ability of the С 60 +Cis complexes to circumvent cancer drug resistance, a molecular docking study was conducted. This analysis demonstrated the potential capability of C 60 fullerene to form van der Waals interactions with potential binding sites of P-gp, MRP-1and MRP-2 (ABCC2) molecules, with maximum affinity to MRP-2. The observed phenomenon might indicate the mechanism how the C 60 +Cis complex bypasses drug resistance of cancer cells by direct binding to ABC transporter proteins. Additionally, the results of Ames mutagenicity test demonstrated that immobilization of Cis on С 60 fullerene significantly diminishes mutagenic activity of Cis and may reduce the probability of secondary neoplasms induction. Concluding, the synthesized C 60 +Cis complex effectively induces cancer cell death in vitro and inhibits tumor growth in vivo, circumventing cancer cell resistance to chemotherapy due to the specific affinity of C 60 fullerene towards ABC-transporter proteins. The obtained results indicate the C 60 +Cis complex as a promising novel chemotherapeutic agentespecially for treatment of drug-resistant tumors.

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Graphene quantum dots enhance anticancer activity of cisplatin via increasing its cellular and nuclear uptake

Cited by 83 publications

References 43 publications

<p>Development of biosurfactant-based graphene quantum dot conjugate as a novel and fluorescent theranostic tool for cancer</p>

<p>Development of biosurfactant-based graphene quantum dot conjugate as a novel and fluorescent theranostic tool for cancer</p>

Cocoa Bean-Extract Mediated Graphene Quantum Dots as Antimicrobial, Anticancer and Plant Growth Regulators

C60 fullerene enhances cisplatin anticancer activity and overcomes tumor cell drug resistance

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