Graphene-protein bioelectronic devices with wavelength-dependent photoresponse

Lu, Ye; Lerner, Mitchell; Qi, Zhengqing John; Mitala, Joseph; Lim, Jong Hsien; Discher, Bohdana M.; Johnson, A. T. Charlie

doi:10.1063/1.3678024

Cited by 42 publications

(42 citation statements)

References 13 publications

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“…47 The resulting MoS 2 –protein hybrid structure is shown schematically in Figure 4b,c. The histidine tag has been used as an anchor site in other biosensor systems, including those based on carbon nanotube 12 and graphene 13 FETs. These previous functionalization schemes relied upon nitrilotriacetic acid to bind Ni ions, resulting in a ~2 nm thick linker layer.…”

Section: Resultsmentioning

confidence: 99%

“…These previous functionalization schemes relied upon nitrilotriacetic acid to bind Ni ions, resulting in a ~2 nm thick linker layer. 13 The MoS 2 functionalization scheme presented here is a simple, one-step surface chemistry that results in an extremely short linker. This is expected to ensure strong electrostatic coupling of target binding events to the MoS 2 channel and should render the devices robust against screening that occurs for measurements in ionic liquids.…”

Section: Resultsmentioning

confidence: 99%

“…In particular, polyhistidine tags (histags), which are routinely used to enable purification of recombinant proteins, have been targeted for this purpose. 10–13 …”

mentioning

confidence: 99%

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Scalable Production of Molybdenum Disulfide Based Biosensors

et al. 2016

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We demonstrate arrays of opioid biosensors based on chemical vapor deposition grown molybdenum disulfide (MoS2) field effect transistors (FETs) coupled to a computationally redesigned, water-soluble variant of the μ-opioid receptor (MOR). By transferring dense films of monolayer MoS2 crystals onto prefabricated electrode arrays, we obtain high-quality FETs with clean surfaces that allow for reproducible protein attachment. The fabrication yield of MoS2 FETs and biosensors exceeds 95%, with an average mobility of 2.0 cm2 V−1 s−1 (36 cm2 V−1 s−1) at room temperature under ambient (in vacuo). An atomic length nickel-mediated linker chemistry enables target binding events that occur very close to the MoS2 surface to maximize sensitivity. The biosensor response calibration curve for a synthetic opioid peptide known to bind to the wild-type MOR indicates binding affinity that matches values determined using traditional techniques and a limit of detection ~3 nM (1.5 ng/mL). The combination of scalable array fabrication and rapid, precise binding readout enabled by the MoS2 transistor offers the prospect of a solid-state drug testing platform for rapid readout of the interactions between novel drugs and their intended protein targets.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Scalable Production of Molybdenum Disulfide Based Biosensors

et al. 2016

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“…Raman spectroscopy measurements showed an increase in the ratio of the intensity of the D band to the G band after diazonium salt treatment, consistent with the creation of covalent bonds to the NT sidewall (see Supplemental Figure 2 in the Supporting Information). 29 …”

Section: Resultsmentioning

confidence: 99%

Hybrids of a Genetically Engineered Antibody and a Carbon Nanotube Transistor for Detection of Prostate Cancer Biomarkers

et al. 2012

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We developed a novel detection method for osteopontin (OPN), a new biomarker for prostate cancer, by attaching a genetically engineered single chain variable fragment (scFv) protein with high binding affinity for OPN to a carbon nanotube field-effect transistor (NTFET). Chemical functionalization using diazonium salts is used to covalently attach scFv to NT-FETs, as confirmed by atomic force microscopy, while preserving the activity of the biological binding site for OPN. Electron transport measurements indicate that functionalized NT-FET may be used to detect the binding of OPN to the complementary scFv protein. A concentration-dependent increase in the source-drain current is observed in the regime of clinical significance, with a detection limit of approximately 30 fM. The scFv-NT hybrid devices exhibit selectivity for OPN over other control proteins. These devices respond to the presence of OPN in a background of concentrated bovine serum albumin, without loss of signal. Based on these observations, the detection mechanism is attributed to changes in scattering at scFv protein-occupied defect sites on the carbon nanotube sidewall. The functionalization procedure described here is expected to be generalizable to any antibody containing an accessible amine group, and to result in biosensors appropriate for detection of corresponding complementary proteins at fM concentrations.

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“…Protein sensors. Lu et al suggested that His-tagged proteins are easily detected by graphene FET (G-FET) biosensors by the binding of Ni ions to the nitrilotriacetic acid (NTA) (Lu et al, 2012). FET based on aptamer-modified graphene easily detected the IgE protein because the reaction between IgE aptamers and IgE protein is highly active; the solution was stirred for several tens of seconds after the addition of each analyte (Ohno et al, 2010b).…”

Section: Graphene-based Fet Biosensorsmentioning

confidence: 99%