Graphene oxide prevents mycobacteria entry into macrophages through extracellular entrapment

Maio, Flavio De; Palmieri, Valentina; Salustri, Alessandro; Perini, Giordano; Sanguinetti, Maurizio; Spirito, Marco De; Delogu, Giovanni; Papi, Massimiliano

doi:10.1039/c8na00413g

Cited by 29 publications

(31 citation statements)

References 55 publications

(80 reference statements)

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“…AFM confirmed the size distribution obtained by DLS. Height distribution of each nanoparticle was measured, and the recorded height is comparable to data reported for graphene [ 7 ].…”

Section: Resultssupporting

confidence: 62%

“…Confocal microscopy measurements of Laurdan and Dox uptake were carried out using an inverted microscope (Nikon A1 MP+, Nikon, Japan) equipped with a 60× oil immersion objective [ 7 ]. Images were acquired at 37 °C for all measurements.…”

Section: Methodsmentioning

confidence: 99%

“…GQDs are fragments of graphene, a single-layer, two-dimensional carbon allotrope [ 5 ]. Graphene-based materials, especially graphene oxide (GO), have been widely employed in several biomedical applications as antibacterial agents [ 6 , 7 , 8 ], as scaffolds for bone regeneration [ 9 , 10 ], and as diagnostic tools [ 11 , 12 ]. Among graphene-based materials, GQDs are emerging as excellent candidates for bioimaging, in theranostic approaches, and, importantly, as drug-delivery agents [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

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Graphene Quantum Dots’ Surface Chemistry Modulates the Sensitivity of Glioblastoma Cells to Chemotherapeutics

Perini

Palmieri

Ciasca

et al. 2020

IJMS

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Recent evidence has shown that graphene quantum dots (GQDs) are capable of crossing the blood–brain barrier, the barrier that reduces cancer therapy efficacy. Here, we tested three alternative GQDs’ surface chemistries on two neural lineages (glioblastoma cells and mouse cortical neurons). We showed that surface chemistry modulates GQDs’ biocompatibility. When used in combination with the chemotherapeutic drug doxorubicin, GDQs exerted a synergistic effect on tumor cells, but not on neurons. This appears to be mediated by the modification of membrane permeability induced by the surface of GQDs. Our findings highlight that GQDs can be adopted as a suitable delivery and therapeutic strategy for the treatment of glioblastoma, by both directly destabilizing the cell membrane and indirectly increasing the efficacy of chemotherapeutic drugs.

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“…AFM confirmed the size distribution obtained by DLS. Height distribution of each nanoparticle was measured, and the recorded height is comparable to data reported for graphene [ 7 ].…”

Section: Resultssupporting

confidence: 62%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Graphene Quantum Dots’ Surface Chemistry Modulates the Sensitivity of Glioblastoma Cells to Chemotherapeutics

Perini

Palmieri

Ciasca

et al. 2020

IJMS

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“…Recently, we examined the effects of GO trapping on Mycobacterium tuberculosis (Mtb), a large rod-shaped bacterium and the etiologic agent of human tuberculosis (TB) [10]. During infection, Mtb is phagocytized by alveolar macrophages, where it can replicate and infect nearby cells and then spread to other tissues [11].…”

Section: Introductionmentioning

confidence: 99%

“…During infection, Mtb is phagocytized by alveolar macrophages, where it can replicate and infect nearby cells and then spread to other tissues [11]. GO traps Mtb extracellularly thereby reducing entry in macrophages [10]. This is of particular interest considering also the GO lung targeting ability when injected intravenously [12].…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide-Linezolid Combination as Potential New Anti-Tuberculosis Treatment

et al. 2020

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Global pandemic management represents a serious issue for health systems. In some cases, repurposing of existing medications might help find compounds that have the unexpected potential to combat microorganisms. In the same way, changing cell–drug interaction by nanotechnology could represent an innovative strategy to fight infectious diseases. Tuberculosis (TB) remains one of the most alarming worldwide infectious diseases and there is an urgent need for new drugs and treatments, particularly for the emergence and spread of drug-resistant Mycobacterium tuberculosis (Mtb) strains. New nanotechnologies based on carbon nanomaterials are now being considered to improve anti-TB treatments, and graphene oxide (GO) showed interesting properties as an anti-TB drug. GO, which preferentially accumulates in the lungs and is degraded by macrophagic peroxidases, can trap Mycobacterium smegmatis and Mtb in a dose-dependent manner, reducing the entry of bacilli into macrophages. In this paper, combinations of isoniazid (INH), amikacin (AMK) and linezolid (LZD) and GO anti-mycobacterial properties were evaluated against Mtb H37Rv by using a checkerboard assay or an in vitro infection model. Different GO effects have been observed when incubated with INH, AMK or LZD. Whereas the INH and AMK anti-mycobacterial activities were blocked by GO co-administration, the LZD bactericidal effect increased in combination with GO. GO-LZD significantly reduced extracellular mycobacteria during infection and was able to kill internalized bacilli. GO-LZD co-administration is potentially a new promising anti-TB treatment at the forefront in fighting emerging antibiotic-resistant Mtb strains where LZD administration is suggested. This innovative pharmacological approach may lead to reduced treatment periods and decreased adverse effects. More importantly, we demonstrate how nanomaterials–drugs combinations can represent a possible strategy to quickly design drugs for pandemics treatment.

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MoS2-based nanostructures for biomedical applications and carbon nanomaterials in TB treatment

Panchu

Anand

Vijayakumar³

et al. 2021

A Mechanistic Approach to Medicines for Tuberculosis Nanotherapy

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Graphene oxide prevents mycobacteria entry into macrophages through extracellular entrapment

Abstract: GO trapping reduces mycobacteria entry in macrophages when the bacilli are in the external environment: a possible therapeutic strategy for tuberculosis.

Cited by 29 publications

References 55 publications

Graphene Quantum Dots’ Surface Chemistry Modulates the Sensitivity of Glioblastoma Cells to Chemotherapeutics

Graphene Quantum Dots’ Surface Chemistry Modulates the Sensitivity of Glioblastoma Cells to Chemotherapeutics

Graphene Oxide-Linezolid Combination as Potential New Anti-Tuberculosis Treatment

MoS2-based nanostructures for biomedical applications and carbon nanomaterials in TB treatment

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