Graphene Oxide-Lignin/Silk Fibroin/ZnO Nanobiocomposite: A Novel Bioactive Scaffold With Antibacterial Activity

Abstract

In this study, a novel nanobiocomposite was synthesized using graphene oxide, lignin, silk fibroin and ZnO and used in biological fields. To synthesize this structure, after preparing graphene oxide by the Hummer method, lignin, silk fibroin, and ZnO nanoparticles (NPs) were added to it, respectively. Also, ZnO NPs with a particle size of about 18 nm to 33 nm was synthesized via Camellia sinensis extract by green methodology. The synthesized structure was examined as anti-biofilm agent and it was observed that the Graphene oxide-lignin/silk fibroin/ZnO nanobiocomposite has a significant ability to prevent the formation of P. aeruginosa biofilm. In addition, due to the importance of the possibility of using this structure in biological environments, its toxicity and blood compatibility were also evaluated. According to the obtained results from MTT assay, the viability percentages of Hu02 cells treated with Graphene oxide-lignin/silk fibroin/ZnO nanobiocomposite after 24, 48, and 72 h of incubation were 89.96%, 89.32%, and 91.28%. On the other hand, the hemolysis percentage of the synthesized structure after 24 h and 72 h of extraction was 9.5% and 11.76% respectively. As a result, the synthesized structure is hemocompatible and had no toxic effects on Hu02 cells.

Fabrication of a novel porous nanostructure based on NiCuFe2O4@MCM-48, embedded with graphene oxide/poly (p-phenylenediamine) to construct an efficient supercapacitor

“…4 C(B). The reduction in mass between 200 and 300 °C can be attributed to the thermal decomposition of oxygen-containing functional groups present in GO, such as carboxyl, epoxide, and hydroxyl groups 12 . Furthermore, the thermal degradation of PρPD polymer chains likely begins at a temperature range of 250 to 340 °C 59 .…”

“…The preparation of graphene oxide involved a modified Hummer method, as described in previous studies 12 . To begin, 1 g of graphite was combined with 23 ml of 98% sulfuric acid in a 1000 ml beaker.…”