Graphene oxide coatings enhance fluorescence signals in a lateral flow immunoassay for the detection of UCH-L1, a marker for trauma brain injury

Natarajan, Satheesh; Joseph, Jayaraj; Prazeres, D.M.F.

doi:10.1016/j.snb.2023.134336

Cited by 3 publications

(1 citation statement)

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“…Experimental results showed that the addition of 80 ng of GO at test regions improved fluorescence signals 2–3-fold. GO can enhance the capture of antibody immobilization efficiency at the test region, resulting in an augmented density of recognition complexes on the test region surface and hence the final signal [ 30 ].…”

Section: Assay Improvementmentioning

confidence: 99%

Lateral Flow Assay: A Summary of Recent Progress for Improving Assay Performance

Omidfar,

Riahi,

Kashanian

2023

Biosensors

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Lateral flow tests are one of the most important types of paper-based point-of-care (POCT) diagnostic tools. It shows great potential as an implement for improving the rapid screening and management of infections in global pandemics or other potential health disorders by using minimally expert staff in locations where no sophisticated laboratory services are accessible. They can detect different types of biomarkers in various biological samples and provide the results in a little time at a low price. An important challenge regarding conventional LFAs is increasing their sensitivity and specificity. There are two main approaches to increase sensitivity and specificity, including assay improvement and target enrichment. Assay improvement comprises the assay optimization and signal amplification techniques. In this study, a summarize of various sensitivity and specificity enhancement strategies with an objective evaluation are presented, such as detection element immobilization, capillary flow rate adjusting, label evolution, sample extraction and enrichment, etc. and also the key findings in improving the LFA performance and solving their limitations are discussed along with numerous examples.

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Section: Assay Improvementmentioning

confidence: 99%