Graphene oxide–cationic polymer conjugates: Synthesis and application as gene delivery vectors

Teimouri, Mohsen; Nia, Azadeh Hashem; Abnous, Khalil; Eshghi, Hossein; Ramezani, Mohammad

doi:10.1016/j.plasmid.2016.03.002

Cited by 49 publications

(36 citation statements)

References 40 publications

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“…Such modifications may include conjugation with alkylated derivatives of different cationic polymers, such as polyethylenimine (PEI), polypropylenimine (PPI), and polyamidoamine (PAMAM), by linkers, such as surface carboxyl group, glycine, and spermidine. The transfection efficiency of these vectors, when evaluated by using green fluorescent protein (GFP) plasmid, PEI–GO conjugate bearing glycine linker, was found to be the most efficient vector [121]. It has also been demonstrated by enhanced green fluorescent protein (EGFP) transfection in HeLa cells that GO with polyethylenimine (PEI) is able to deliver genes inside the cells with mitigated cytotoxicity and improved transfection efficiency [116].…”

Section: Other Functional Vectorsmentioning

confidence: 99%

The Development of Functional Non-Viral Vectors for Gene Delivery

Patil

Gao

Lin

et al. 2019

IJMS

194

135

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Gene therapy is manipulation in/of gene expression in specific cells/tissue to treat diseases. This manipulation is carried out by introducing exogenous nucleic acids, such as DNA or RNA, into the cell. Because of their negative charge and considerable larger size, the delivery of these molecules, in general, should be mediated by gene vectors. Non-viral vectors, as promising delivery systems, have received considerable attention due to their low cytotoxicity and non-immunogenicity. As research continued, more and more functional non-viral vectors have emerged. They not only have the ability to deliver a gene into the cells but also have other functions, such as the performance of fluorescence imaging, which aids in monitoring their progress, targeted delivery, and biodegradation. Recently, many reviews related to non-viral vectors, such as polymers and cationic lipids, have been reported. However, there are few reviews regarding functional non-viral vectors. This review summarizes the common functional non-viral vectors developed in the last ten years and their potential applications in the future. The transfection efficiency and the transport mechanism of these materials were also discussed in detail. We hope that this review can help researchers design more new high-efficiency and low-toxicity multifunctional non-viral vectors, and further accelerate the progress of gene therapy.

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Section: Other Functional Vectorsmentioning

confidence: 99%

The Development of Functional Non-Viral Vectors for Gene Delivery

Patil

Gao

Lin

et al. 2019

IJMS

194

135

View full text Add to dashboard Cite

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“…several studies have modified the graphene derivatives by polymers such as polyethylenimine (PEI), [143][144][145][146] lactosylated chitosan oligosaccharide (LCO), chitosan (CS), [147][148][149] and polyamidoamine (PAMAM). 150,151 For instance, the results of Zhang's studies 113 clearly showed that the use of PEI-conjugated GO to deliver DOX and siRNA does not only lead to improved therapy efficiency but also to patient safety increment.…”

Section: Dovepressmentioning

confidence: 99%

“…Based on conjugation of GO and PEI, PAMAM, and polypropylenimine (PPI), three groups of vectors have been fabricated. 145 They have also used glycine, surface carboxyl group, and spermidine as linkers in their study. Their invaluable results showed that the synthesized vectors generally have lower cytotoxicity in comparison with the intact polymer.…”

Section: Dovepressmentioning

confidence: 99%

<p>Applications of Graphene and Graphene Oxide in Smart Drug/Gene Delivery: Is the World Still Flat?</p>

Hoseini‐Ghahfarokhi¹,

Mirkiani²,

Mozaffari³

et al. 2020

IJN

150

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Graphene, a wonder material, has made far-reaching developments in many different fields such as materials science, electronics, condensed physics, quantum physics, energy systems, etc. Since its discovery in 2004, extensive studies have been done for understanding its physical and chemical properties. Owing to its unique characteristics, it has rapidly became a potential candidate for nano-bio researchers to explore its usage in biomedical applications. In the last decade, remarkable efforts have been devoted to investigating the biomedical utilization of graphene and graphene-based materials, especially in smart drug and gene delivery as well as cancer therapy. Inspired by a great number of successful graphene-based materials integrations into the biomedical area, here we summarize the most recent developments made about graphene applications in biomedicine. In this paper, we review the up-to-date advances of graphene-based materials in drug delivery applications, specifically targeted drug/ gene delivery, delivery of antitumor drugs, controlled and stimuli-responsive drug release, photodynamic therapy applications and optical imaging and theranostics, as well as investigating the future trends and succeeding challenges in this topic to provide an outlook for future researches.

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“…GO possesses a high photothermal conversion capability, especially in the near-infrared wavelength region. The GO can produce a strong absorption and heat transfer effect from light, and this heat could kill tumor cells directly [133,134]. Due to this photothermal effect, GO has also been used in several biomedical applications, including cancer therapy.…”

Section: Fr-mediated Graphene-based Nanoparticlesmentioning

confidence: 99%

Folate Receptor-Mediated siRNA Delivery: Recent Developments and Future Directions for RNAi Therapeutics

Gangopadhyay

Nikam

Gore

2021

Nucleic Acid Therapeutics

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RNA interference (RNAi), a gene regulatory process mediated by small interfering RNAs (siRNAs), has made remarkable progress as a potential therapeutic agent against various diseases. However, RNAi is associated with fundamental challenges such as poor systemic delivery and susceptibility to the nucleases. Targeting ligand-bound delivery vehicles has improved the accumulation of drug at the target site, which has resulted in high transfection efficiency and enhanced gene silencing. Recently, folate receptor (FR)-mediated targeted delivery of siRNAs has garnered attention due to their enhanced cellular uptake and high transfection efficiency toward tumor cells. Folic acid (FA), due to its small size, low immunogenicity, high in vivo stability, and high binding affinity toward FRs, has attracted much attention for targeted siRNA delivery. FRs are overexpressed in a large number of tumors, including ovarian, breast, kidney, and lung cancer cells. In this review, we discuss recent advances in FA-mediated siRNA delivery to treat cancers and inflammatory diseases. This review summarizes various FA-conjugated nanoparticle systems reported so far in the literature, including liposome, silica, metal, graphene, dendrimers, chitosan, organic copolymers, and RNA nanoparticles. This review will help in the design and development of potential delivery vehicles for siRNA drug targeting to tumor cells using an FR-mediated approach.

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Graphene oxide–cationic polymer conjugates: Synthesis and application as gene delivery vectors

Cited by 49 publications

References 40 publications

The Development of Functional Non-Viral Vectors for Gene Delivery

The Development of Functional Non-Viral Vectors for Gene Delivery

<p>Applications of Graphene and Graphene Oxide in Smart Drug/Gene Delivery: Is the World Still Flat?</p>

Folate Receptor-Mediated siRNA Delivery: Recent Developments and Future Directions for RNAi Therapeutics

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