Graphene oxide based electrochemical label free immunosensor for rapid and highly sensitive determination of tumor marker HSP70

Özcan, Burcu; Sezgintürk, Mustafa Kemal

doi:10.1016/j.talanta.2016.07.039

Cited by 32 publications

(11 citation statements)

References 36 publications

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“…The increase in the real and the imaginary parts of the impedance and thus the impedance modulus (|Z|) confirms the BSA’s immobilization on all electrodes, which causes an increase in the charge transfer resistance. , The lowest value of the impedance module registered for the lowest frequency (0.1 Hz) was noted for the TNT sample and, what is essential, the |Z| value was precisely the same for both immobilization times, which proves the complete saturation of the TNT layer only after 0.5 h of the protein deposition. Similar to TNTs, comparable changes in impedance parameters for both immobilization times are recorded for the 10sd_AgNPs/TNT electrode, which is why it was considered that half an hour is sufficient to saturate this platform.…”

Section: Resultsmentioning

confidence: 99%

Electrodes Based on a Titanium Dioxide Nanotube–Spherical Silver Nanoparticle Composite for Sensing of Proteins

Nycz

Arkusz

Pijanowska

2020

ACS Biomater. Sci. Eng.

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The aim of the research was to provide electrochemical, chemical, phase, and microscopic characteristics of electrodes based on titanium dioxide nanotubes (TNTs) containing uniformly deposited, nonagglomerated spherical silver nanoparticles (AgNPs). The nanoparticles were produced with the use of electrodeposition and sputter deposition methods. This paper presents the results of research of these platforms with the use of the following techniques: electrochemical impedance spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, and scanning electron microscopy. Evaluation of the adsorption of proteinsbovine serum albumin (BSA)was carried out to establish the possibility of the use of the electrodes in a low-cost, simple detection system without surface functionalization. The research proved that the AgNP deposition facilitated the electron transfer increasing their conductivity properties as well as promoting the protein adsorption. The AgNPs/TNT electrodes showed a high selectivity to the BSA–anti-BSA complex. Half an hour of immobilization was enough to completely saturate the TNT electrodes, whereas for AgNPs/TNTs, 1 h of immobilization seemed to be not enough. The impedance parameter changes for electrodes with the AgNPs reached even about 300%. The biggest changes were noted for the platform obtained using cyclic voltammetry, so it is the best detection platform for biosensing.

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Section: Resultsmentioning

confidence: 99%

Electrodes Based on a Titanium Dioxide Nanotube–Spherical Silver Nanoparticle Composite for Sensing of Proteins

Nycz

Arkusz

Pijanowska

2020

ACS Biomater. Sci. Eng.

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“…5(a). As can be seen, at the bare ions bearing negative charge and deaccelerate the diffusion process of redox probe to the GCE surface [62]. Compared to curve b, an obvious expansion in the capacity of the anodic and cathodic currents was observed when the rGO was casted on GCE/GO (curve c) due to its good conductivity and large active surface area of rGO.…”

Section: Electrochemical Behavior Of the Hbsag Aptasensormentioning

confidence: 91%

Design of Ultrasensitive Electrochemical Aptasensor for Earlier Detection of Hepatitis B Virus

Mohsin¹,

Mashkour²,

Fatemi³

et al. 2021

Nano BioMed ENG

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In consideration of the imperious need for developing a simple, ultra-sensitive and selective method to detect Hepatitis B virus (HBV), an label free aptasensor was constructed by the modification of a chemically inert glassy carbon electrode (GCE) by dint of a gold nanoparticle immobilized reduced graphene oxide. The ultrasensitive detection was attained using a specific ssDNA (thiolated aptamer at the 5′ end) as a recognition element. The aptamer was fastened onto GCE/rGO/Au surface via the Au-S bond for determining the impedance of charge mobility by means of [Fe(CN) 6 ] 3-/4as a redox gauge. The function of aptasensor based on the specific interaction between the aptamer and specific surface antigen HBsAg induced [HBsAg-aptamer] complex formation which impeded electron movability between a [Fe(CN) 6 ] 3-/4redox probe solution and sensing interface. HBsAgbinding aptamer events can be easily monitored by the signal switch through cyclic voltammetry technique (CV), square wave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS) measurements. Under the optimized conditions, the aptasensor can detect HBsAg in the 0.25 fg/mL to 1.5 fg/mL concentration range and limit of detection of 0.018, 0.0016 and 0.001 fg/mL for CV, SWV and EIS respectively. The recovery of 88-109.6% was obtained. Moreover, the selectivity of the aptasensor was tested using other biological interfering substances. This developed aptasensor provided one of the lowest limits of detection for HBsAg recently reported in the literature, satisfying stability and good reproducibility, consequently could be applied for revealing HBsAg in real samples.

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“… 102 Several research teams have made great efforts to detect lymphoma and MM based on the electrochemical biosensors. 76 – 79 , 87 …”

Section: Applications Of Electrochemical Biosensors In Lymphoma and MMmentioning

confidence: 99%

“…Moreover, Geetha et al 78 developed a novel electrochemical immunosensor for FR detection, adopting FA-modified RGO nanosheets as the substrate. Through the covalent immobilization of HSP70 antibodies on GO as a bio-recognition element, Ozcan and Sezginturk 79 proposed a label-free immunosensor to analyze HSP70 via electrochemical methods such as CV and AC impedance. All of these sensors used for the detection of biomolecules related to lymphoma or MM have achieved relatively satisfactory results, suggesting their great potentiality in the early evaluation of tumor diseases.…”

Section: Applications Of Electrochemical Biosensors In Lymphoma and MMmentioning

confidence: 99%

Applications of electrochemical biosensors based on 2D materials and their hybrid composites in hematological malignancies diagnosis

Sun

Huang

Wang

et al. 2022

Technol Cancer Res Treat

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Hematological malignancies encompass a wide variety of severe diseases that pose a serious threat to human health. Given the fact that hematological malignancies are difficult to treat due to their unpredictable and rapid deterioration and high rates of recurrence, growing attention has been paid to their early screening and diagnosis. However, developing a rapid and effective diagnostic tool featuring a noninvasive sampling technique is still extremely challenging. In recent years, novel nanomaterials-based electrochemical biosensors have attracted great interest because of such advantages as simple operation, low cost, fast response, etc. As a kind of rising nanomaterials, two-dimensional materials have excellent electronic and chemical properties, which have been proven to improve the performance of electrochemical biosensors. This review summarizes the applications of different types of electrochemical biosensors (nucleic acid sensors, immunosensors, aptamer biosensors, and cytosensors) based on two-dimensional materials in the detection of biological molecules related to hematological malignancies. Two-dimensional materials-based electrochemical biosensors designed for the diagnosis of leukemia could rapidly detect the target biomolecules at a trace level and show great merits such as wide linear range, low detection limit, high sensitivity, excellent selectivity, and cost-effectiveness. In addition, these biosensors have also achieved satisfactory results in the diagnosis of lymphoma and multiple myeloma. Thus, two-dimensional materials-based electrochemical biosensors are attractive for the early diagnosis of hematological malignancies in clinical practice. Nevertheless, more efforts are still required to further improve the performance of electrochemical biosensors. In this review, we propose the possible main concerns in the design of future two-dimensional materials-based electrochemical biosensors, involving the development of sensors for synchronous detection of diverse target biomolecules, the exploration of other superior two-dimensional materials, the simplification of the sensors fabrication process, the construction of new hybrid structures and how to avoid possible environmental issues.

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Graphene oxide based electrochemical label free immunosensor for rapid and highly sensitive determination of tumor marker HSP70

Cited by 32 publications

References 36 publications

Electrodes Based on a Titanium Dioxide Nanotube–Spherical Silver Nanoparticle Composite for Sensing of Proteins

Electrodes Based on a Titanium Dioxide Nanotube–Spherical Silver Nanoparticle Composite for Sensing of Proteins

Design of Ultrasensitive Electrochemical Aptasensor for Earlier Detection of Hepatitis B Virus

Applications of electrochemical biosensors based on 2D materials and their hybrid composites in hematological malignancies diagnosis

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