Graphene oxide based electrochemical immunosensor for rapid detection of groundnut bud necrosis orthotospovirus in agricultural crops

Chaudhary, Mohit; Verma, Shilpi; Kumar, Ashwani; Basavaraj, Y. B.; Tiwari, Pratibha; Singh, S. N.; Chauhan, Sunil; Kumar, Pushpendra; Singh, Surinder P.

doi:10.1016/j.talanta.2021.122717

Cited by 22 publications

(12 citation statements)

References 26 publications

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“…Two absorption peaks at 236 nm and 300 nm were observed and assigned to the π-π* (C=C) and n-π* (C=O) electronic transitions in the GO sheets. This indicates the formation of GO nanosheets [ 50 ]. The disappearance of the C=O peak in the UV-Vis spectra of rGO signifies the reduction of GO to rGO.…”

Section: Resultsmentioning

confidence: 99%

Clinically Deployable Bioelectronic Sensing Platform for Ultrasensitive Detection of Transferrin in Serum Sample

Kaur

Chittineedi

Bellala³

et al. 2023

Biosensors

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Varying levels of transferrin (Tf) have been associated with different disease conditions and are known to play a crucial role in various malignancies. Regular monitoring of the variations in Tf levels can be useful for managing related diseases, especially for the prognosis of certain cancers. We fabricated an immunosensor based on graphene oxide (GO) nanosheets to indirectly detect Tf levels in cancer patients. The GO nanosheets were deposited onto an indium tin oxide (ITO)-coated glass substrate and annealed at 120 °C to obtain reduced GO (rGO) films, followed by the immobilization of an antibody, anti-Tf. The materials and sensor probe used were systematically characterized by UV–Visible spectroscopy (UV–Vis), X-ray diffraction (XRD), atomic force microscopy (AFM), and Fourier transform infrared spectroscopy (FTIR). Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV) were also used for the stepwise sensor probe characterizations and Tf detection in serum samples, respectively. The anti-Tf/rGO/ITO immunosensor DPV output demonstrated an excellent Tf detection capability in the linear range of 0.1 mg mL−1 to 12 mg mL−1 compared to the enzyme-linked immunosorbent assay (ELISA) detection range, with a limit of detection (LOD) of 0.010 ± 0.007 mg mL−1. Furthermore, the results of the fabricated immunosensor were compared with those of the ELISA and autobioanalyzer techniques, showing an outstanding match with < 5% error and demonstrating the immunosensor’s clinical potential.

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Section: Resultsmentioning

confidence: 99%

Clinically Deployable Bioelectronic Sensing Platform for Ultrasensitive Detection of Transferrin in Serum Sample

Kaur

Chittineedi

Bellala³

et al. 2023

Biosensors

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“…Figure 2(b) shows the redox current and standard square difference recorded by CV of six electrodes in [Fe(CN)6] 4-/3- before and after AuNPs modification. It can be seen that AuNPs-modified electrodes not only have higher current but also show better uniformity [ 28 ]. This is also important for subsequent EBV detection.…”

Section: Resultsmentioning

confidence: 99%

Point-of-Care Based Electrochemical Immunoassay for Epstein-Barr Virus Detection

Miao

Liu

2022

Journal of Analytical Methods in Chemistry

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This work describes a label-free electrochemical immunosensor for the sensing of Epstein-Barr virus (EBV) with high sensitivity. First, a monolayer of 1,6-hexanedithiol (HDT) was fabricated on the screen-printed electrode surface by the interaction between sulfur atoms and SPE. AuNPs can be modified on the electrode by the Au-S bond formed between the HDT-free group and Au atom in AuNPs. Protein A is then adsorbed onto AuNPs. Several parameters were optimized. The optimum concentration of protein A is 0.6 mg/mL. The optimum immobilization time for protein A is 90 min. The optimum concentration of antibody is 80 μg/mL. The optimum immobilization time for antibody is 90 min. Directional immobilization of EBV antibody is achieved by high affinity binding of protein A to the Fc segment of antibody. When antigen specifically binds to antibody, the formation of immune complexes blocks electron transfer of [Fe(CN)6]4-/3- and is reflected in the detection of cyclic voltammetry/electrochemical impedance spectroscopy. The detection range is 1 pg/mL–l00 ng/mL with a LOD of 0.1 pg/mL. In addition, the proposed sensor exhibited an excellent antiinterference property.

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“…The process for the preparation of the Ab 2 label is shown in Scheme 1(A). Indeed, based on the literature, 27,28 we used a concentration of 0.1 M EDC/NHS (1 : 4) for coupling amino groups to carboxyl groups (acidic conditions 4.5-6). Firstly, 500 mL Ab 2 conjugate was stirred with the prepared 0.1 M EDC/NHS (1 : 4) for 1.5 h at 4 1C.…”

Section: Preparation Of Antibody Markermentioning

confidence: 99%

Application of a CuS/Au heterostructure with peroxidase-like activity in immunosensors

Wang

Jiang

et al. 2022

New J. Chem.

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Graphene oxide based electrochemical immunosensor for rapid detection of groundnut bud necrosis orthotospovirus in agricultural crops

Cited by 22 publications

References 26 publications

Clinically Deployable Bioelectronic Sensing Platform for Ultrasensitive Detection of Transferrin in Serum Sample

Clinically Deployable Bioelectronic Sensing Platform for Ultrasensitive Detection of Transferrin in Serum Sample

Point-of-Care Based Electrochemical Immunoassay for Epstein-Barr Virus Detection

Application of a CuS/Au heterostructure with peroxidase-like activity in immunosensors

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