Graphene oxide assists polyvinylidene fluoride scaffold to reconstruct electrical microenvironment of bone tissue

Shuai, Cijun; Zeng, Zichao; Qi, Fangwei; Peng, Shuping; Yang, Wenjing; He, Chongxian; Wang, Guoyong; Qian, Guowen

doi:10.1016/j.matdes.2020.108564

Cited by 100 publications

(59 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using stem cell self‐renewal and differentiation ability to treat osteoporosis may be the most potential way of bone repair, which is expected to solve the medical problems of bone regeneration and repair 29 . In recent years, biological scaffolds have made rapid progress 30‐33 . It is particularly urgent to clarify the mechanism of stem cell differentiation and develop new strategies to induce stem cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

Linc02349 promotes osteogenesis of human umbilical cord‐derived stem cells by acting as a competing endogenous RNA for miR‐25‐3p and miR‐33b‐5p

et al. 2020

Self Cite

View full text Add to dashboard Cite

Objectives: Increasing evidences suggest that inducing mesenchymal stem cells to differentiate into osteoblasts has been as an especially important component in the prevention and therapy for degenerative bone disease. Here, we identify a novel lncRNA, linc02349, which increases significantly during osteogenic differentiation. Materials and methods:Human umbilical cord-derived stem cells (hUC-MSCs) and dental pulp mesenchymal stem cells were used. Overexpression and knockdown of linc02349 in cell lines were generated using lentiviral-mediated gene delivery method.Bioinformatics prediction, Ago2-RIP assay and dual-luciferase reporter system were employed to examine miRNA which interacts with linc02349. The RNA FISH assay was performed to identify the subcelluar location of linc02349. Alizarin Red S staining, ALP staining and qPCR were applied to identify the osteogenic differentiation. The potential linc02349-regulated genes, miR-25-3p and miR-33b-5p, were explored by ChIP, RIP and Western blotting assays. Micro-CT was used to measure the osteogenic content in bone formation assay in vivo. Results: Linc02349 overexpression improves osteogenic differentiation by in vitroand in vivo analysis. Mechanistically, linc02349 acts as a molecular sponge for miR-25-3p and miR-33b-5p to control expression abundance of SMAD5 and Wnt10b,

show abstract

Section: Discussionmentioning

confidence: 99%

Linc02349 promotes osteogenesis of human umbilical cord‐derived stem cells by acting as a competing endogenous RNA for miR‐25‐3p and miR‐33b‐5p

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, GO could be functionalized to further improve its electrical conductivity. 94,95 For example, Jin et al 94 introduced GO into the polyvinyl chloride/poly(lactic-co-glycolic acid) (PVC/PLGA) scaffold by electrospinning, and then GO-PVC/PLGA 96 composite scaffolds were immersed in hydrogen iodide solution to obtain rGO-PVC/PLGA scaffolds. The results showed that compared with GO-PVC/PLGA scaffolds, rGO-PVC/PLGA scaffolds had higher capacitance and better conductivity at a suitable voltage, which was due to the larger SSA of rGO-PVC/PLGA and the formation of more conjugated π-bonds on the surface of rGO.…”

Section: Excellent Electrical Propertiesmentioning

confidence: 99%

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

Du¹,

Wang

Zhang³

et al. 2020

IJN

View full text Add to dashboard Cite

During continuous innovation in the preparation, characterization and application of various bone repair materials for several decades, nanomaterials have exhibited many unique advantages. As a kind of representative two-dimensional nanomaterials, graphene and its derivatives (GDs) such as graphene oxide and reduced graphene oxide have shown promising potential for the application in bone repair based on their excellent mechanical properties, electrical conductivity, large specific surface area (SSA) and atomic structure stability. Herein, we reviewed the updated application of them in bone repair in order to present, as comprehensively, as possible, their specific advantages, challenges and current solutions. Firstly, how their advantages have been utilized in bone repair materials with improved bone formation ability was discussed. Especially, the effects of further functionalization or modification were emphasized. Then, the signaling pathways involved in GDs-induced osteogenic differentiation of stem cells and immunomodulatory mechanism of GDs-induced bone regeneration were discussed. On the other hand, their applications as contrast agents in the field of bone repair were summarized. In addition, we also reviewed the progress and related principles of the effects of GDs parameters on cytotoxicity and residues. At last, the future research was prospected.

show abstract

“…Compared between groups, the difference between GO + MSC group, MSC group and GO group was not statistically signi cant (P > 0.05), and the difference between blank group was statistically signi cant (P < 0.05) ( Table 1). 1) 21.020 ± 0.026 2) 17.624 ± 0.127 3) Group B 23.662 ± 0.056 20.544 ± 0.085 4) 19.424 ± 0.046 5) 17.799 ± 0.049 6) P value 0.029 0.430 0.254 0.725…”

Section: Serum Nomentioning

confidence: 97%

“…Recently, the signi cant advantages of graphene oxides (GO) used as cell scaffold materials have attracted tremendous interests due to their large surface areas, friendly biological compatibility and good hydrophility. [1][2][3][4][5] As an ideal seed cell in tissue engineering, mesenchymal stem cell (MSC) has been widely used in the eld of regenerative medicine. [6][7][8][9][10][11] MSC has the effect of regulating local immunity and improving the in ammatory environment.…”

Section: Introductionmentioning

confidence: 99%

Effects of Co-Culture of Graphene Oxide Scaffolds with Different Concentrations and Umbilical Mesenchymal Stem Cells on the Proliferation and Differentiation of Stem Cells

Liu

Gong

Wang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background The main role of the scaffold materials is to enable cells to survive in the scaffold binding as while as to further promote their proliferation and differentiation ability. For mesenchymal stem cell, the scaffold could provide an environment for them to maintain their phenotype, and synthesize all necessary molecules and proteins. Generally, scaffold materials for stem cell need to possess basic characteristics such as high porosity, large surface area, surface rigidity and biodegradability. Thus, the two-dimensional graphene oxide (GO) with oxygen-containing functional groups may be suitable scaffold materials for mesenchymal stem cell culture. Methods In this study, the effect of GO on the value-added differentiation activity of mesenchymal stem cell was systematically investigated. Results It was found that low concentration of GO and sufficient concentration of umbilical cord mesenchymal stem cells are suitable for the second Co-culture. Furthermore, the addition of hyaluronic acid will make this culture more evenly distributed. Conclusions The adsorption of GO on umbilical cord mesenchymal stem cells can also make the two closely linked, which avoids the impact of animal joint activities on cells.

show abstract

Graphene oxide assists polyvinylidene fluoride scaffold to reconstruct electrical microenvironment of bone tissue

Cited by 100 publications

References 49 publications

Linc02349 promotes osteogenesis of human umbilical cord‐derived stem cells by acting as a competing endogenous RNA for miR‐25‐3p and miR‐33b‐5p

Linc02349 promotes osteogenesis of human umbilical cord‐derived stem cells by acting as a competing endogenous RNA for miR‐25‐3p and miR‐33b‐5p

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

Effects of Co-Culture of Graphene Oxide Scaffolds with Different Concentrations and Umbilical Mesenchymal Stem Cells on the Proliferation and Differentiation of Stem Cells

Contact Info

Product

Resources

About