Graphene Oxide and Lipid Membranes: Size-Dependent Interactions

Frost, Rickard; Svedhem, Sofia; Langhammer, Christoph; Kasemo, Bengt Herbert

doi:10.1021/acs.langmuir.5b03239

Cited by 40 publications

(24 citation statements)

References 41 publications

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“…27,65 Furthermore, recent work using supported lipid membranes has pointed to a role of the lateral dimensions of GO for lipid membrane interactions. 66 However, direct experimental evidence of lipid extraction and/or lipid oxidation in membranes of bacterial or mammalian cells has been lacking. By using confocal microscopy and SEM, we were able to show that GO, specifically large GO sheets, disrupted lipid raft domains in neutrophils and triggered NETs.…”

Section: Discussionmentioning

confidence: 99%

Graphene Oxide Elicits Membrane Lipid Changes and Neutrophil Extracellular Trap Formation

Mukherjee

Lazzaretto

Hultenby

et al. 2018

Chem

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Graphene oxide (GO) is a promising material for a variety of biomedical and other applications. The increasing use of GO necessitates careful assessment of potential health hazards. Using primary neutrophils as a model, Mukherjee et al. show that GO elicits neutrophil extracellular traps. Furthermore, by using ToF-SIMS, the authors noted pronounced perturbations of plasma membrane lipids in cells exposed to GO.

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Section: Discussionmentioning

confidence: 99%

Graphene Oxide Elicits Membrane Lipid Changes and Neutrophil Extracellular Trap Formation

Mukherjee

Lazzaretto

Hultenby

et al. 2018

Chem

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“…[142] To understand how lipids interact with GO on solid substrates, GO has been incubated in the presence of various lipid compositions. [143,144] Fig. 4c shows a lipid membrane that is first formed on a SiO 2 substrate by vesicle fusion assembly.…”

Section: Graphene Lipid Superstructures: Towards Graphene Bioelectronicsmentioning

confidence: 99%

Sensing at the Surface of Graphene Field‐Effect Transistors

et al. 2016

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Abstract. 10Recent research trends now offer new opportunities for developing the next generations of label-free biochemical sensors using graphene and other two-dimensional materials. While the physics of graphene transistors operated in electrolyte is well grounded, important chemical challenges still remain to be addressed, namely the impact of the chemical functionalizations of graphene on the key electrical parameters and the sensing performances. In fact, graphene -at least ideal graphene -is highly chemically inert. The 15 functionalizations and chemical alterations of the graphene surface -both covalently and non-covalently -are crucial steps that define the sensitivity of graphene. The presence, reactivity, adsorption of gas and ions, proteins, DNA, cells and tissues on graphene have been successfully monitored with graphene. This review aims to unify most of the work done so far on biochemical sensing at the surface of a (chemically functionalized) graphene field-effect transistor and the challenges that lie ahead. We are convinced that graphene biochemical sensors 20 hold great promises to meet the ever-increasing demand for sensitivity, especially looking at the recent progresses suggesting that the obstacle of Debye screening can be overcome.2

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“…A better understanding of the physical principles governing these complex interactions can be achieved by using simple and reproducible systems, such as model lipid membranes. Several experimental studies have been recently performed on the effects of graphene-based materials acting on simple solid supported lipid membranes [14,15], unilamellar lipid vesicles [15,16] and liposomes [16,17]. The affinity of graphene and graphene oxides for the hydrophobic region of the lipid membranes gives rise to a variety of modes of interaction that include the formation of multilayered graphene/membrane structures, the generation of pores in vesicle membranes, the insertion of graphene into liposomes or the complete destruction of the bilayer configuration [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Interaction modes between nanosized graphene flakes and liposomes: Adsorption, insertion and membrane fusion

Santiago

Reigada

2019

Biochimica et Biophysica Acta (BBA) - General Subjects

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Background: Understanding the effects of graphene-based nanomaterials on lipid membranes is critical to determine their environmental impact and their efficiency in the biomedical context. Graphene has been reported to favourably interact with biological and model lipid membranes. Methods: We report on a systematic coarse-grained molecular dynamics study of the interaction modes of graphene nanometric flakes with POPC/cholesterol liposome membranes. We have simulated graphene layers with a variety of sizes and oxidation degrees, and we have analyzed the trajectories, the interaction modes, and the energetics of the observed phenomena. Results: Three interaction modes are reported. Graphene can be transiently adsorbed onto the liposome membrane and/or inserted in its hydrophobic region. Inserted nanosheets prefer a perpendicular orientation, and tilt in order to maximize the contact with phospholipid tails while avoiding the contact with cholesterol molecules. When placed between two liposomes, graphene facilitates their fusion in a single vesicle. Conclusions: Graphene can be temporary adsorbed on the liposome before insertion. Bilayer curvature has an influence on the orientation of inserted graphene particles. Cholesterol molecules are depleted from the surrounding of graphene particles. Graphene layers may catalyse membrane fusion by bypassing the energy barrier required in stalk formation. General significance: Nanometric graphene layers can be adsorbed/inserted in lipid-based membranes in different manners and affect the cholesterol distribution in the membrane, implying important consequences on the structure and functionality of biological cell membranes, and on the bioaccumulation of graphene in living organisms. The graphene-mediated mechanism opens new possibilities for vesicle fusion in the experimental context.

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Graphene Oxide and Lipid Membranes: Size-Dependent Interactions

Cited by 40 publications

References 41 publications

Graphene Oxide Elicits Membrane Lipid Changes and Neutrophil Extracellular Trap Formation

Graphene Oxide Elicits Membrane Lipid Changes and Neutrophil Extracellular Trap Formation

Sensing at the Surface of Graphene Field‐Effect Transistors

Interaction modes between nanosized graphene flakes and liposomes: Adsorption, insertion and membrane fusion

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