Graphene Oxide—A Tool for the Preparation of Chemically Crosslinking Free Alginate–Chitosan–Collagen Scaffolds for Bone Tissue Engineering

Kolanthai, Elayaraja; Sindu, P. Abinaya; Khajuria, Deepak Kumar; Veerla, Sarath Chandra; Kuppuswamy, Dhandapani; Catalani, Luiz H.; Mahapatra, D. Roy

doi:10.1021/acsami.8b00699

Cited by 162 publications

(100 citation statements)

References 63 publications

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“…The spatial structure of the triple helices and collagen crystallinity could be represented by the XRD diffraction pattern [38]. In Figure 5, A-CF and P-CF show similar peak shapes with COL.…”

Section: X-ray Diffractionmentioning

confidence: 96%

Comparison of the Structural Characteristics of Native Collagen Fibrils Derived from Bovine Tendons Using Two Different Methods: Modified Acid-Solubilized and Pepsin-Aided Extraction

Liu

Zhang

et al. 2020

Materials

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Native collagen fibrils (CF) were successfully extracted from bovine tendons using two different methods: modified acid-solubilized extraction for A-CF and pepsin-aided method for P-CF. The yields of A-CF and P-CF were up to 64.91% (±1.07% SD) and 56.78% (±1.22% SD) (dry weight basis), respectively. The analyses of both amino acid composition and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that A-CF and P-CF were type I collagen fibrils. Both A-CF and P-CF retained the intact crystallinity and integrity of type I collagen’s natural structure by FTIR spectra, circular dichroism spectroscopy (CD) and X-ray diffraction detection. The aggregation structures of A-CF and P-CF were displayed by UV–Vis. However, A-CF showed more intact aggregation structure than P-CF. Microstructure and D-periodicities of A-CF and P-CF were observed (SEM and TEM). The diameters of A-CF and P-CF are about 386 and 282 nm, respectively. Although both A-CF and P-CF were theoretically concordant with the Schmitt hypothesis, A-CF was of evener thickness and higher integrity in terms of aggregation structure than P-CF. Modified acid-solubilized method provides a potential non-enzyme alternative to extract native collagen fibrils with uniform thickness and integral aggregation structure.

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Section: X-ray Diffractionmentioning

confidence: 96%

Comparison of the Structural Characteristics of Native Collagen Fibrils Derived from Bovine Tendons Using Two Different Methods: Modified Acid-Solubilized and Pepsin-Aided Extraction

Liu

Zhang

et al. 2020

Materials

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“…The abilities to print biocompatible, tissue-specific matrix scaffolds with GO, and to incorporate seed cells, biological proteins, and 3D-printed scaffolds have been applied in a wide variety of fields, particularly in tissue engineering and orthopedic applications [11,12]. An articular cartilage matrix has a low intrinsic repair capacity due to its lack of blood vessels as well as its sparse cell population.…”

Section: Introductionmentioning

confidence: 99%

Potential use of 3D-printed graphene oxide scaffold for construction of the cartilage layer

Zhong

Diao

et al. 2020

J Nanobiotechnol

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Background: Three-dimensional (3D) printing involves the layering of seed cells, biologically compatible scaffolds, and biological activity factors to precisely recapitulate a biological tissue. Graphene oxide (GO), a type of micro material, has been utilized as a small molecule-transport vehicle. With the proliferation of GO, the biocompatibility of chondrocytes in a microenvironment constructed by 3D printed scaffolds and GO is innovative. Accordingly, we speculate that, as a type of micro material, GO can be used with 3D scaffolds for a uniform distribution in the cartilage layer. Results: A qualitative analysis of the chondrocyte-proliferation potential revealed that the culture of 3D printing with a 10% GO scaffold was higher than that of the other groups. Meanwhile, the progress of cell apoptosis was activated. Through scanning electron microscopy, immunofluorescence, and in vivo research, we observed that the newborn cartilage matrix extended along the border of the cartilage and scaffold and matured. After an analysis with immunohistochemical staining with aggrecan and collagen I, the cartilage following the 3D-printed scaffold was thinner than that of the 3D-printed GO scaffold. Furthermore, the collagen I of the cartilage expression in treatment with the GO scaffold was significant from week 2 to 6. Conclusions: The findings indicate that a 3D-printed GO scaffold can potentially be utilized for the construction of a cartilage matrix. However, the optimum concentration of GO requires further research and discussion.

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“…The micropore volumes was found to be 0.34 cm 3 g −1 for CMPCA-1 and 0.22 cm 3 g −1 for CMPCA-2 by using t-plot method, respectively. Besides, the porosity of the CMPCAs measured by using liquid displacement procedure [33] was found to be 92.1% for CMPCA-1 and 91.7% for CMPCA-2, indicating excellent porous feature of the CMPs-based carbon aerogels. Owing to their abundant porosity, the as-prepared CMPs-based carbon aerogels possess desired heat-insulated property.…”

mentioning

confidence: 96%

Superwetting Monolithic Hollow‐Carbon‐Nanotubes Aerogels with Hierarchically Nanoporous Structure for Efficient Solar Steam Generation

Zhang

Bai

et al. 2018

Advanced Energy Materials

371

232

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hydrogen, [2] solar power plants, [3] photovoltaic cells, [4] photocatalysis, [5] and water desalination, [6] the photothermal materials based solar water evaporation is one of the most promising approaches for harvesting and conversion of solar energy. Solar vapor generation, more specifically, is a surface water evaporation process in which the light is absorbed and converted to heat energy by photothermal materials to generate vapor. Compared with the common water evaporation by solar radiation as heat source which suffers from the drawback of low solar energy conversion efficiency due to the fact that the part solar energy is converted to heat bulk water or is lost to the external environment, solar vapor generation based on photothermal materials has great advantages for its high light-to-heat conversion efficiency due to the fact that solar radiation is only harvested and located at the water-air interface to heat thin air-water surface layer that can effectively minimize the heat loss. [7] Based on the merits mentioned above, up to now, the solar steam generator has been emerged as a kind of efficient device for harvesting solar energy and attracted extensively much more attention in both industrial and academic research throughout the past decades. [8,9] In a given solar steam generation system, the photothermal materials is essential. A desired photothermal material should meet the following criteria: the broadband sunlight absorbability, low thermal conductivity, open porosity for rapid water molecules transportation, and high-energy conversion efficiency. [10,11] Understanding of these complementary roles of these parameters for photothermal material, so far, a number of photothermal materials, including carbon-based materials, [7,8,[12][13][14] metallic nanoparticles, [15][16][17] biomass-based materials, [18,19] and porous polymers, [20,21] etc., have been developed to use as efficient solar steam generators.In general, porous materials with bilayer structure are widely adapted as solar steam generator, in which the top layer consists of carbon materials for light absorption (e.g., graphene, [13] CNTs, [14] graphite, [7] etc.) while the bottom layer is composed by the porous materials (e.g., wood, [18] silica, [10] etc.) for Solar steam generation has been proven to be one of the most efficient approaches for harvesting solar energy for diverse applications such as distillation, desalination, and production of freshwater. Here, the synthesis of monolithic carbon aerogels by facile carbonization of conjugated microporous polymer nanotubes as efficient solar steam generators is reported. The monolithic carbon-aerogel networks consist of randomly aggregated hollow-carbon-nanotubes (HCNTs) with 100-250 nm in diameter and a length of up to several micrometers to form a hierarchically nanoporous network structure. Treatment of the HCNTs aerogels with an ammonium peroxydisulfate/sulfuric acid solution endows their superhydrophilic wettability which is beneficial for rapid transportation of water molecules. ...

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Graphene Oxide—A Tool for the Preparation of Chemically Crosslinking Free Alginate–Chitosan–Collagen Scaffolds for Bone Tissue Engineering

Cited by 162 publications

References 63 publications

Comparison of the Structural Characteristics of Native Collagen Fibrils Derived from Bovine Tendons Using Two Different Methods: Modified Acid-Solubilized and Pepsin-Aided Extraction

Comparison of the Structural Characteristics of Native Collagen Fibrils Derived from Bovine Tendons Using Two Different Methods: Modified Acid-Solubilized and Pepsin-Aided Extraction

Potential use of 3D-printed graphene oxide scaffold for construction of the cartilage layer

Superwetting Monolithic Hollow‐Carbon‐Nanotubes Aerogels with Hierarchically Nanoporous Structure for Efficient Solar Steam Generation

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