Graphene nanoplatelets based matrix solid-phase dispersion microextraction for phenolic acids by ultrahigh performance liquid chromatography with electrochemical detection

Abstract

A simple, rapid and eco-friendly approach based on matrix solid-phase dispersion microextraction (MSPDM) followed by ultrahigh performance liquid chromatography coupled with electrochemical detection (UHPLC-ECD) was presented for the microextraction and determination of six phenolic acids in a plant preparation (Danshen tablets). The parameters that influenced the extraction performance of phenolic acids were investigated and optimized. The optimal MSPDM conditions were determined as follows: sorbent, using graphene nanoplatelets with sample/sorbent ratio of 1:1, grinding time set at 60 s, and 0.2 mL of water as elution solvent. Under the optimum conditions, the validation experiments indicated that the proposed method exhibited good linearity (r2 ≥ 0.9991), excellent precision (RSD ≤ 4.57%), and satisfactory recoveries (82.34–98.34%). The limits of detection were from 1.19 to 4.62 ng/mL for six phenolic acids. Compared with other reported methods, this proposal required less sample, solvent and extraction time. Consequently, the proposed method was successfully used to the extraction and determination of phenolic acids in Danshen tablets.

Production of graphene nanoplate/polyetheretherketone composites by semi-industrial melt-compounding

“…Considering the high cost of graphene, GNPs, which are formed from several stacked graphene layers bound to each other by Van der Waals forces, are an interesting option for large-scale production [12] of nanocomposite materials. High thermal conductivity (5000 W/mK) [13], high thermal stability in the range of 360-500 C [14], and elastic modulus (%1 TPa) [13] of GNPs are among the highest of the available large-scale carbon nanofillers [15,16,17].…”

The Potential of Microextraction Techniques for the Analysis of Bioactive Compounds in Food

“…This was achieved, for instance, by coupling magnetic particles with molecular imprinting, as reported in the extraction of different phenolic compounds from fruits and juices ( 58 , 59 ). But eventually, the major innovation in microextraction of bioactive compounds in food analysis is the use of new and more efficient sorbents, allowing greater retention capacities, as a 2D-ultrathin Ni/Co-NO 3 layered double hydroxide nanosheet ( 46 ) or graphene nanoplatelets ( 71 ) to extract phenolic acids from fruit juices and plant preparations. Also, carbon nanofibers (CNFs) were reported to extract phytohormones and ginsenosides ( 69 ).…”

Evaluation of the phytochemicals and antioxidant activity of Lophatherum gracile Brongn based on chemical fingerprinting by HPLC with electrochemical detection

“…Indeed, electrochemistry is an effective means for evaluating the antioxidant activity of a species, wherein it has been documented that there is a significant correlation between the DPPH scavenging activity and the peak height or the first half-wave potential [27,28]. Phenolic acids and flavonoids tend to exhibit superior response values by HPLC-electrochemical detection (HPLC-ECD) than compounds that do not bear phenolic hydroxyl groups [26,29]. Since the leaves of L. gracile contain various phenolic acids and flavonoids, it was considered that a good correlation may exist between the chromatographic peak area of L. gracile leaves and their antioxidant activity.…”