Graphene nanogap electrodes in electrical biosensing

Terse-Thakoor, Trupti; Ramnani, Pankaj; Villarreal, Claudia C.; Yan, Dong; Tran, Thien-Toan; Pham, Tung; Mulchandani, Ashok

doi:10.1016/j.bios.2018.11.049

Cited by 15 publications

(12 citation statements)

References 38 publications

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“…The electrodes showed high-affinity interactions of streptavidin–gold nanoparticles to the biotin-functionalized nanogaps. This platform is recommended as a biosensor for the detection of other affinity-based biomolecular interactions, such as nucleic acid, antigen–antibody, or chemo-selective interactions [ 111 ]. In another study, nanogap interdigitated electrode (IDE) arrays with assisted gold nanoparticles were used to enhance the sensitivity of detection [ 85 ].…”

Section: Nanogap Electrodesmentioning

confidence: 99%

Electrochemical Impedance Spectroscopy (EIS): Principles, Construction, and Biosensing Applications

Magar

Hassan

Mulchandani

2021

Sensors

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522

238

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Electrochemical impedance spectroscopy (EIS) is a powerful technique used for the analysis of interfacial properties related to bio-recognition events occurring at the electrode surface, such as antibody–antigen recognition, substrate–enzyme interaction, or whole cell capturing. Thus, EIS could be exploited in several important biomedical diagnosis and environmental applications. However, the EIS is one of the most complex electrochemical methods, therefore, this review introduced the basic concepts and the theoretical background of the impedimetric technique along with the state of the art of the impedimetric biosensors and the impact of nanomaterials on the EIS performance. The use of nanomaterials such as nanoparticles, nanotubes, nanowires, and nanocomposites provided catalytic activity, enhanced sensing elements immobilization, promoted faster electron transfer, and increased reliability and accuracy of the reported EIS sensors. Thus, the EIS was used for the effective quantitative and qualitative detections of pathogens, DNA, cancer-associated biomarkers, etc. Through this review article, intensive literature review is provided to highlight the impact of nanomaterials on enhancing the analytical features of impedimetric biosensors.

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Section: Nanogap Electrodesmentioning

confidence: 99%

Electrochemical Impedance Spectroscopy (EIS): Principles, Construction, and Biosensing Applications

Magar

Hassan

Mulchandani

2021

Sensors

Self Cite

522

238

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“…Terse-Thakoor et al, designed an electrical biosensor using a streptavidin-biotin system, graphene nanogap electrodes, and gold nanoparticles (102).…”

Section: Formation Of the Avidin-biotin Complexmentioning

confidence: 99%

Nucleic acid-based biosensors: analytical devices for prevention, diagnosis and treatment of diseases

Barbosa

Cepeda

León-Torres

et al. 2021

Vitae

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BACKGROUND : Biosensing techniques have been the subject of exponentially increasing interest due to their performance advantages such as high selectivity and sensitivity, easy operation, low cost, short analysis time, simple sample preparation, and real-time detection. Biosensors have been developed by integrating the unique specificity of biological reactions and the high sensitivity of physical sensors. Therefore, there has been a broad scope of applications for biosensing techniques, and nowadays, they are ubiquitous in different areas of environmental, healthcare, and food safety. Biosensors have been used for environmental studies, detecting and quantifying pollutants in water, air, and soil. Biosensors also showed great potential for developing analytical tools with countless applications in diagnosing, preventing, and treating diseases, mainly by detecting biomarkers. Biosensors as a medical device can identify nucleic acids, proteins, peptides, metabolites, etc.; these analytes may be biomarkers associated with the disease status. Bacterial food contamination is considered a worldwide public health issue; biosensor-based analytical techniques can identify the presence or absence of pathogenic agents in food. OBJECTIVES: The present review aims to establish state-of-the-art, comprising the recent advances in the use of nucleic acid-based biosensors and their novel application for the detection of nucleic acids. Emphasis will be given to the performance characteristics, advantages, and challenges. Additionally, food safety applications of nucleic acid-based biosensors will be discussed. METHODS: Recent research articles related to nucleic acid-based biosensors, biosensors for detecting nucleic acids, biosensors and food safety, and biosensors in environmental monitoring were reviewed. Also, biosensing platforms associated with the clinical diagnosis and food industry were included. RESULTS: It is possible to appreciate that multiple applications of nucleic acid-based biosensors have been reported in the diagnosis, prevention, and treatment of diseases, as well as to identify foodborne pathogenic bacteria. The use of PNA and aptamers opens the possibility of developing new biometric tools with better analytical properties. CONCLUSIONS: Biosensors could be considered the most important tool for preventing, treating, and monitoring diseases that significantly impact human health. The aptamers have advantages as biorecognition elements due to the structural conformation, hybridization capacity, robustness, stability, and lower costs. It is necessary to implement biosensors in situ to identify analytes with high selectivity and lower detection limits.

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“…By contrast, electrochemical sensors have a number of adventitious properties that make them particularly suitable for point-of-use applications. They are highly sensitive and portable, are relatively low cost, exhibit a rapid time-to-result, are highly versatile e.g., different, electrode materials (C, Au, Pt) are suitable for a variety of different chemical molecules, and may be modified with a large variety of biomolecules for bio-sensing applications [ 21 , 22 , 23 , 24 ]. Furthermore, they are low power and provide an electrical transduction signal, which may be fed directly into electronic systems (without conversion) amplified, undergo edge analytics with the resultant data sent to the cloud [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Advanced Solid State Nano-Electrochemical Sensors and System for Agri 4.0 Applications

Seymour¹,

Narayan²,

Creedon³

et al. 2021

Sensors

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Global food production needs to increase in order to meet the demands of an ever growing global population. As resources are finite, the most feasible way to meet this demand is to minimize losses and improve efficiency. Regular monitoring of factors like animal health, soil and water quality for example, can ensure that the resources are being used to their maximum efficiency. Existing monitoring techniques however have limitations, such as portability, turnaround time and requirement for additional reagents. In this work, we explore the use of micro- and nano-scale electrode devices, for the development of an electrochemical sensing platform to digitalize a wide range of applications within the agri-food sector. With this platform, we demonstrate the direct electrochemical detection of pesticides, specifically clothianidin and imidacloprid, with detection limits of 0.22 ng/mL and 2.14 ng/mL respectively, and nitrates with a detection limit of 0.2 µM. In addition, interdigitated electrode structures also enable an in-situ pH control technique to mitigate pH as an interference and modify analyte response. This technique is applied to the analysis of monochloramine, a common water disinfectant. Concerning biosensing, the sensors are modified with bio-molecular probes for the detection of both bovine viral diarrhea virus species and antibodies, over a range of 1 ng/mL to 10 µg/mL. Finally, a portable analogue front end electronic reader is developed to allow portable sensing, with control and readout undertaken using a smart phone application. Finally, the sensor chip platform is integrated with these electronics to provide a fully functional end-to-end smart sensor system compatible with emerging Agri-Food digital decision support tools.

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Graphene nanogap electrodes in electrical biosensing

Cited by 15 publications

References 38 publications

Electrochemical Impedance Spectroscopy (EIS): Principles, Construction, and Biosensing Applications

Electrochemical Impedance Spectroscopy (EIS): Principles, Construction, and Biosensing Applications

Nucleic acid-based biosensors: analytical devices for prevention, diagnosis and treatment of diseases

Advanced Solid State Nano-Electrochemical Sensors and System for Agri 4.0 Applications

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