Graphene-incorporated chitosan substrata for adhesion and differentiation of human mesenchymal stem cells

Abstract

A simple method that uses graphene to fabricate nanotopographic substrata was reported for stem cell engineering. Graphene-incorporated chitosan substrata promoted adhesion and differentiation of human mesenchymal stem cells (hMSCs). In addition, we proposed that nanotopographic cues of the substrata could enhance cell-cell and cell-material interactions for promoting functions of hMSCs.

In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material

“…24,25 A large number of studies have shown that graphene-reinforced composites can promote the adhesion, growth, and osteogenic differentiation of stem cells. [26][27][28] Therefore, this study added graphene as a toughening agent to nHA/PA66 to improve the mechanical properties and bioactivity of the composite materials. The influence of graphene on the adhesion, proliferation, and differentiation of MSCs was also investigated.…”

“…These results may be due to the unique nanotopography of the graphene-reinforced polymer composites increasing the expression of various integrins and connexins in MSCs. 27,28 Another mechanism of graphene that can promote osteogenic differentiation is its provision of a concentrated platform for the chemical substances required for the induction of osteogenic differentiation. 44 Strong π-π stacking allows graphene to adsorb chemicals consisting of benzene rings, such as β-glycerophosphate and dexamethasone.…”

Multifunctional, biocompatible and pH-responsive carbon nanotube- and graphene oxide/tectomer hybrid composites and coatings

“…Combining the 2D structures of both tectomers and GO is very appealing for a range of applications, in particular for certain bio-applications that require high surface area materials to act as coatings for implants or as scaffold to enhance biocompatibility, mechanical, and other properties. [82][83][84][85] Here we investigate the interactions between the tectomers and GO with the intention of achieving GO-based biocomposites. For this study, we used well-exfoliated and stable GO dispersions in water.…”

Effects of Micro-Electrical Stimulation on Regulation of Behavior of Electro-Active Stem Cells

“…Animal or human stem cells are characterized by their unique ability to differentiate into various types of cells, yielding an important key in the regenerative medicine including animal tissue regeneration (Gronthos et al 2000;Jo et al 2007;Richardson et al 2007;Wang et al 2011;Kim et al 2013). It is therefore important to obtain a large number of stem cells and develop technology for regulating them for stem cellbased biological engineering applications.…”

“…It is therefore important to obtain a large number of stem cells and develop technology for regulating them for stem cellbased biological engineering applications. Stem cells are exposed on various physical and biochemical environments that enable them to regulate or maintain appropriate biological functions including proliferation in animal or human systems (Gronthos et al 2000;Jo et al 2007;Richardson et al 2007;Wang et al 2011;Kim et al 2013;. In general, the traditional biologists have focused on the behavior of stem cells through 'cocktail' of biochemical factors such as proteins and hormones ).…”