Graphene-Based Sensor for the Detection of Cortisol for Stress Level Monitoring and Diagnostics

Zubarev, Alexei; Cuzminschi, Marina; Iordache, Ana‐Maria; Iordache, Stefan Marian; Rizea, C.; Grigorescu, C.E.A.; Giuglea, Carmen

doi:10.3390/diagnostics12112593

Cited by 10 publications

(5 citation statements)

References 56 publications

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“…Additionally, the interactions between cortisol and the layer were simulated using Marvin Beans ® software (https://chemaxon.com/marvin, accessed on 30 October 2023). The results demonstrated a notable degree of sensitivity in the measurement of salivary cortisol levels using cyclic voltammetry, with the capability to detect cortisol concentrations as low as 0.5 ng/mL [123].…”

Section: Applications In Cortisol Detectionmentioning

confidence: 93%

“…Figure 11 shows that this particular technology exhibits potential suitability for utilization as a wearable electrochemical sensor for the detection of cortisol in human sweat when integrated into a watchstrap [122]. Zubarev et al investigated the sensing characteristics of multi-layer graphene in conjunction with pyrrole to develop an affordable and highly sensitive material for the detection of cortisol [123]. Pyrrole and graphene nanoplatelets were dispersed in 1 M HNO 3 with the utilization of an intense ultrasound probe for a duration of 10 min.…”

Section: Applications In Cortisol Detectionmentioning

confidence: 99%

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Electrochemical Detection of Hormones Using Nanostructured Electrodes

Haroon,

Stine

2023

Coatings

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Hormones regulate several physiological processes in living organisms, and their detection requires accuracy and sensitivity. Recent advances in nanostructured electrodes for the electrochemical detection of hormones are described. Nanostructured electrodes’ high surface area, electrocatalytic activity, and sensitivity make them a strong hormone detection platform. This paper covers nanostructured electrode design and production using MOFs, zeolites, carbon nanotubes, metal nanoparticles, and 2D materials such as TMDs, Mxenes, graphene, and conducting polymers onto electrodes surfaces that have been used to confer distinct characteristics for the purpose of electrochemical hormone detection. The use of aptamers for hormone recognition is producing especially promising results, as is the use of carbon-based nanomaterials in composite electrodes. These materials are optimized for hormone detection, allowing trace-level quantification. Various electrochemical techniques such as SWV, CV, DPV, EIS, and amperometry are reviewed in depth for hormone detection, showing the ability for quick, selective, and quantitative evaluation. We also discuss hormone immobilization on nanostructured electrodes to improve detection stability and specificity. We focus on real-time monitoring and tailored healthcare with nanostructured electrode-based hormone detection in clinical diagnostics, wearable devices, and point-of-care testing. These nanostructured electrode-based assays are useful for endocrinology research and hormone-related disease diagnostics due to their sensitivity, selectivity, and repeatability. We conclude with nanotechnology–microfluidics integration and tiny portable hormone-detection devices. Nanostructured electrodes can improve hormone regulation and healthcare by facilitating early disease diagnosis and customized therapy.

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Section: Applications In Cortisol Detectionmentioning

confidence: 93%

Section: Applications In Cortisol Detectionmentioning

confidence: 99%

Electrochemical Detection of Hormones Using Nanostructured Electrodes

Haroon,

Stine

2023

Coatings

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“…The material used for immobilizing enzymes must have good biocompatibility and not cause enzyme inactivation. CTS, as a cheap and easy-to-obtain material, is often used to enhance the immobilization of enzymes and electrodes because its chemical structure contains many N and O functional groups that serve as covalent modification starting points [ 28 ], and the material is easy to obtain and cheap. Therefore, this paper used CTS as the adhesive for enzymes.…”

Section: Methodsmentioning

confidence: 99%

An Energy-Efficient Flexible Multi-Modal Wireless Sweat Sensing System Based on Laser Induced Graphene

Feng

Jiang

Wang

et al. 2023

Sensors

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Real-time sweat monitoring is vital for athletes in order to reflect their physical conditions, quantify their exercise loads, and evaluate their training results. Therefore, a multi-modal sweat sensing system with a patch-relay-host topology was developed, which consisted of a wireless sensor patch, a wireless data relay, and a host controller. The wireless sensor patch can monitor the lactate, glucose, K+, and Na+ concentrations in real-time. The data is forwarded via a wireless data relay through Near Field Communication (NFC) and Bluetooth Low Energy (BLE) technology and it is finally available on the host controller. Meanwhile, existing enzyme sensors in sweat-based wearable sports monitoring systems have limited sensitivities. To improve their sensitivities, this paper proposes a dual enzyme sensing optimization strategy and demonstrates Laser-Induced Graphene (LIG)-based sweat sensors decorated with Single-Walled Carbon Nanotubes (SWCNT). Manufacturing an entire LIG array takes less than one minute and costs about 0.11 yuan in materials, making it suitable for mass production. The in vitro test result showed sensitivities of 0.53 μA/mM and 3.9 μA/mM for lactate and glucose sensing, and 32.5 mV/decade and 33.2 mV/decade for K+ and Na+ sensing, respectively. To demonstrate the ability to characterize personal physical fitness, an ex vivo sweat analysis test was also performed. Overall, the high-sensitivity lactate enzyme sensor based on SWCNT/LIG can meet the requirements of sweat-based wearable sports monitoring systems.

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“…Additionally, they may have sensitivity, selectivity, and cost-effectiveness limitations. Electrochemical methods have emerged as promising alternatives for cortisol detection due to their inherent advantages . These methods offer high sensitivity and selectivity, allowing for accurate cortisol detection even at low concentrations.…”

Section: Introductionmentioning

confidence: 99%

“…Electrochemical methods have emerged as promising alternatives for cortisol detection due to their inherent advantages. 8 These methods offer high sensitivity and selectivity, allowing for accurate cortisol detection even at low concentrations. They also demonstrate fast response times, enabling real-time or near-real-time monitoring of cortisol levels.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Cortisol by Silver Nanoparticle-Modified Molecularly Imprinted Polymer-Coated Pencil Graphite Electrodes

et al. 2023

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The sensitive cortisol detection by an electrochemical sensor based on silver nanoparticle-doped molecularly imprinted polymer was successfully improved. This study describes the method development for cortisol detection in both aqueous solution and biological samples using molecularly imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(l)-histidine methyl ester)-coated pencil graphite electrodes modified with silver nanoparticles (AgNPs) by differential pulse voltammetry (DPV). The cortisol-imprinted pencil graphite electrode (PGE) has a large surface area because of doped AgNPs with enhanced electroactivity. The prepared molecularly imprinted polymer was characterized by scanning electron microscopy. The DPV response of the synthesized electrode with outstanding electrical conductivity was clarified. Cortisol-imprinted polymer-coated PGEs (MIP), cortisol-imprinted polymer-coated PGEs with AgNPs (MIP@AgNPs), and nonimprinted polymer-coated PGEs with AgNPs (NIP@AgNPs) were evaluated for sensitive and selective detection of cortisol in aqueous solution. Five different cortisol concentrations (0.395, 0.791, 1.32, 2.64, and 3.96 nM) were applied to the MIP@AgNPs, and signal responses were detected by the DPV with a regression coefficient (R 2) value of 0.9951. The modified electrode showed good electrocatalytic activity toward cortisol for the linear concentration range from 0.395 to 3.96 nM, and a low limit of detection was recorded as 0.214 nM. The results indicate that the MIP@AgNPs sensor has great potential for sensitive and selective cortisol determination in biological samples.

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Graphene-Based Sensor for the Detection of Cortisol for Stress Level Monitoring and Diagnostics

Cited by 10 publications

References 56 publications

Electrochemical Detection of Hormones Using Nanostructured Electrodes

Electrochemical Detection of Hormones Using Nanostructured Electrodes

An Energy-Efficient Flexible Multi-Modal Wireless Sweat Sensing System Based on Laser Induced Graphene

Electrochemical Detection of Cortisol by Silver Nanoparticle-Modified Molecularly Imprinted Polymer-Coated Pencil Graphite Electrodes

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