Graphene-Based High-Efficiency Surface-Enhanced Raman Scattering-Active Platform for Sensitive and Multiplex DNA Detection

He, Songnian; Liu, Keng-Ku; Su, Shao; Yan, Juan; Mao, Xiuhai; Wang, Dongfang; He, Yao; Li, Lain‐Jong; Song, Shiping; Chen, Fan

doi:10.1021/ac300577d

Cited by 186 publications

(124 citation statements)

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“…While complex and laborious procedures are needed for coupling graphene to nanostructured surfaces, 7,8,17 GO coating can be simply obtained by physisorption of GO sheets in…”

Section: ■ Introductionmentioning

confidence: 99%

“…While complex and laborious procedures are needed for coupling graphene to nanostructured surfaces, 7,8,17 GO coating can be simply obtained by physisorption of GO sheets in aqueous solution. 18−21 Beside obvious advantages of this approach, a detailed investigation on how the assembly of GO should be conducted to control adsorption geometry and optical properties at the interface is still lacking.…”

Section: ■ Introductionmentioning

confidence: 99%

“…13−15 Recent findings also sustained the convenient use of GO and of mild reduced GO in generating SERS signals that accurately resemble the native spectrum of the molecules in solution without frequency shifts, which instead are typically generated as a consequence of the interaction with graphene or metal. 16 Overall, veiling plasmonic nanoparticles with GO sheets is perceived as a favorite strategy to fabricate advanced SERS substrates which hold the promise of improved sensitivity, signal stability, and reproducibility.While complex and laborious procedures are needed for coupling graphene to nanostructured surfaces, 7,8,17 GO coating can be simply obtained by physisorption of GO sheets in …”

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confidence: 99%

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Controlled Veiling of Silver Nanocubes with Graphene Oxide for Improved Surface-Enhanced Raman Scattering Detection

Banchelli

Tiribilli

Angelis

et al. 2016

ACS Appl. Mater. Interfaces

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Hybrid graphene oxide (GO)/metal nanocomposites have been recently proposed as novel surface-enhanced Raman scattering (SERS) substrates. Despite an increasing interest in these systems, standardization in their fabrication process is still lacking but urgently required to support their use for real-life applications. In this work we investigate how the assembly of GO should be conducted to control adsorption geometry and optical properties at the interface with plasmonic nanostructures as monolayer assemblies of silver nanocubes, by tuning main experimental parameters including GO concentration and self-assembly time. We finally identified the experimental conditions for building up a close-fitting soft dressing of the plasmonic surface, which shows optimal characteristics for flexible and reliable SERS detection. KEYWORDS: plasmonic nanoparticles, sensing, self-assembly, Raman spectroscopy, quartz crystal microbalance ■ INTRODUCTIONSurface-enhanced Raman scattering (SERS) spectroscopy is as a robust and versatile analytical technique with the ability to offer detailed and label-free chemical information with molecular selectivity and ultrasensitivity. 1−5 The SERS effect relies on huge electromagnetic fields concentrated at the metal surface of a plasmonic nanostructure, which dramatically enhance the Raman signal of molecules placed in its close proximity. In this respect, efficient SERS enhancements are typically generated within less than 10 nm from the metal surface and are mostly confined within the so-called "hot spots", i.e. highly curved nanoregions or gaps and junctions between adjacent nanoparticles. The ability to gather molecules in these regions in a homogeneous and reproducible manner represents a current impediment that is still preventing further diffusion of SERS as a systematic analytical tool.A recent trend toward upgrading the performance of SERS technology relies on the combination of plasmonic nanostructures made of silver and gold with graphene. 6−9 Graphene is a single-atom-thick sheet of sp 2 -hybridized carbon atoms arranged in a honeycomb lattice. A number of unique chemical, electronic, optical, and structural properties make this material a preferred choice for greatly improving electronic and photonic devices. 10,11 With a focus on SERS, current efforts are taking advantage of a thin graphene coating in drawing and concentrating target molecules as well as in conferring the plasmonic surface with a passivating layer that discards possible disturbances and signal variability induced by metal−molecule interactions. 12 Main fabrication methods of graphene sheets include mechanical peel-off, epitaxial growth, and chemical vapor deposition (CVD), which, however, appear unsuitable for large-scale production of graphene, ultimately limiting its practical application. A convenient alternative is now represented by graphene oxide (GO), which is derived by oxidation of graphite in the form of graphene sheets with abundant oxygen-containing functionalities. These confer chemical st...

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“…While complex and laborious procedures are needed for coupling graphene to nanostructured surfaces, 7,8,17 GO coating can be simply obtained by physisorption of GO sheets in…”

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Controlled Veiling of Silver Nanocubes with Graphene Oxide for Improved Surface-Enhanced Raman Scattering Detection

Banchelli

Tiribilli

Angelis

et al. 2016

ACS Appl. Mater. Interfaces

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“…Based on the unique structure of graphene and its π-π interaction with DNA, other methods, such as colorimetry [75][76][77], SERS [78], chemiluminescence [79], and mass spectrometry (MS) [80], have been applied to sensitive and multiplex DNA detection. Taking SERS for instance, researches have been carried out to study the Raman spectrum of graphene [81,82] and its role in Raman enhancement [83].…”

Section: Other Detection Mannersmentioning

confidence: 99%

“…Though it is not clear for the precise origin and further investigation is still needed, one might expand these properties for practical application. Fabricating high-quality graphene with CVD and combining the electromagnetic enhancement of Au nanoparticles, He and coworkers developed the gold-decorated graphene, serving as a highly efficient SERS-active substrate, to facilitate the immobilization of dye-labeled DNAs [78]. A low detection limit of 10 pM was obtained.…”

Section: Other Detection Mannersmentioning

confidence: 99%

Graphene for DNA Biosensing

Han

et al. 2014

SpringerBriefs in Molecular Science

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Graphene (or GO) is an excellent candidate for biomolecules anchoring and detection due to its large surface area (up to 2,630 m 2 /g) and unique sp 2 (sp 2 /sp 3 )-bonded network. According to the binding affinity difference between single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) to graphene sheet, GO has been successfully adopted as a platform to discriminate DNA sequences. Fluorescent, electrochemical, electrical, surface-enhanced Raman scattering (SERS) and other methods have been utilized to achieve the sensitive, selective, and accurate DNA recognition. Both theoretical and experimental results illustrate that ssDNA sequences are adsorbed on the surface of graphene sheet with all nucleobases lying nearly flat. DNA or RNA sequencing through graphene nanopore, nanogap, and nanoribbon has also attracted much interest because it is a label-free, amplification-free, and single-molecule approach that can be scaled up for high-throughput DNA or RNA analysis. Meanwhile, miRNA detection is achieved by forming DNA-miRNA duplex helixes, and strong emission is observed due to the poor interaction between the helix and GO.Keywords DNA sequencing Á miRNA detection Á Fluorescent spectroscopy Á Electrochemical method Á Electrical method Á Graphene nanopore Á Graphene nanogap Á Graphene nanoribbon Investigation of DNA and Graphene Binding InteractionThe large surface area (up to 2,630 m 2 /g) and unique sp 2 (sp 2 /sp 3 )-bonded network make graphene (GO) an excellent candidate for biomolecules anchoring and detection. Taking DNA for the first example, various strategies have been adopted to nucleic acid sequence recognition [1][2][3][4][5][6][7][8][9][10]. To better illustrate the interaction, how nucleobases interact with graphene should be addressed, which may be inspired by the interaction of nucleobases with CNTs [11-13] and highly oriented pyrolytic graphite (HOPG) [14].

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Simultaneous Detection of Diverse Glycoligand‐Receptor Recognitions Using a Single‐Excitation, Dual‐Emission Graphene Composite

Chen

et al. 2015

Adv Funct Materials

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Ligand–protein interactions (LPIs) are fundamental biological processes that manipulate a variety of cellular events. While multiple LPIs can occur concurrently or concertedly at a cellular interface, techniques which are able to simultaneously probe these diverse interactions remain challenging. Here, to the best of our knowledge, the first fluorogenic composite material (FCM) is developed that can probe diverse LPIs at a single biomimetic interface. We determined that two glycoligands coupled with fluorescence dyes with different emission colors can coassemble to a graphene oxide platform, producing an integrated FCM with a quenched fluorescence. The fluorescence of each “glycodye” is uniquely elicited upon interaction with a pairing protein (lectin) that selectively recognizes the glycoligand. Importantly, the dual emission of the FCM can be produced, on a single excitation, while both proteins exist, providing a concise means for the simultaneous probing of diverse LPIs.

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Graphene-Based High-Efficiency Surface-Enhanced Raman Scattering-Active Platform for Sensitive and Multiplex DNA Detection

Cited by 186 publications

References 47 publications

Controlled Veiling of Silver Nanocubes with Graphene Oxide for Improved Surface-Enhanced Raman Scattering Detection

Controlled Veiling of Silver Nanocubes with Graphene Oxide for Improved Surface-Enhanced Raman Scattering Detection

Graphene for DNA Biosensing

Simultaneous Detection of Diverse Glycoligand‐Receptor Recognitions Using a Single‐Excitation, Dual‐Emission Graphene Composite

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