Graphene Based FET Biosensor for Organic-Phosphorous Sample Detection and the Enzymatic Analysis

Abstract

Our paper presents a flexible enzymatic acetylcholinesterase graphene based FET biosensor of the target organic phosphorous. The sensor’s purpose is to detect pesticide residues in the field of food safety. In our sensor design, the material is graphene with its functionalization, and graphene based FET structure will be discussed in one section of this paper. The mechanism of this graphene sensor is the enzymatic linked reaction on a sensor surface. The enzyme is fixed on the sensor surface by the linker 3-mercapto propionic acid. Measurement experiments using the biosensor were performed for detecting the concentration of isocarbophos (an organophosphate). The enzymatic biosensor has successfully detected 100 μg/mL isocarbophos from the water sample, presenting a significant detection limit index for organophosphate detection.