Graphene- and aptamer-based electrochemical biosensor

Xu, Ke; Meshik, Xenia; Nichols, Barbara; Zakar, Eugene; Dutta, Mitra; Stroscio, Michael A.

doi:10.1088/0957-4484/25/20/205501

Cited by 34 publications

(28 citation statements)

References 27 publications

(35 reference statements)

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“…This increases the electron current efficiency that provides the basis for detection for target molecules. It is also worth noting that there is no significant shift in the Dirac point which is the minimum 3 Journal of Sensors conductance point in the device transfer curve, which indicates that the nor p-type property of the graphene does not change with IgE [22]. The volume of IgE in PBS, initial current (I 0 ) (when no gate voltage is applied), Dirac voltage, and minimum conduction current are shown in Table 2. 3.2.…”

Section: Binding Aptamer On Monolayermentioning

confidence: 96%

Detection of Immunoglobulin E with a Graphene-Based Field-Effect Transistor Aptasensor

et al. 2018

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DNA aptamers have the ability to bind to target molecules with high selectivity and therefore have a wide range of clinical applications. Herein, a graphene substrate functionalized with a DNA aptamer is used to sense immunoglobulin E. The graphene serves as the conductive substrate in this field-effect-transistor-like (FET-like) structure. A voltage probe in an electrolyte is used to sense the presence of IgE as a result of the changes in the charge distribution that occur when an IgE molecule binds to the IgE DNA-based aptamer. Because IgE is an antibody associated with allergic reactions and immune deficiency-related diseases, its detection is of utmost importance for biomedical applications.

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Section: Binding Aptamer On Monolayermentioning

confidence: 96%

Detection of Immunoglobulin E with a Graphene-Based Field-Effect Transistor Aptasensor

et al. 2018

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“…For this purpose, it is necessary to functionalize the graphene surface with appropriate receptors, which are antibodies in most cases. Alternate receptors such as antibody fragments and aptamers have also been utilized for protein detection . The detection of the binding of analyte on to the functionalized graphene surface is carried out in many cases using field‐effect.…”

Section: Miniaturized Bioelectronics On‐chipmentioning

confidence: 99%

Bioelectronics and Interfaces Using Monolayer Graphene

et al. 2018

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Graphene is expected to revolutionize several application areas ranging from portable energy conversion and storage to miniaturized biosensors for medical applications. In this endeavor, the control of surface characteristics is an essential aspect for understanding fundamental phenomena occurring at the graphene‐liquid interface. In this comprehensive review, we address recent progress in the investigation of the interfacial characteristics of monolayer graphene and methods for modulating the physical and chemical properties of this interface. We focus on the electrochemistry and field‐effect measurements in liquid, which provide an improved understanding of the unique graphene‐liquid interface, with due consideration of the influence of the underlying substrate and structural defects in graphene. Finally, we present reported examples of using single graphene monolayers in miniaturized devices for realizing sensors, neural interfaces and batteries.

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“…Unlike the conventional metal-oxide semiconductor field-effect transistors (MOSFETs), where the conducting channel is buried in the bulk material, the nanotubes or graphene are located on the surface of the supporting substrate in direct contact with the environment. (Liu and Guo, 2012;Xu et al, 2014) Upon incubation and specific binding of chemical/biological analytes, the conductance changes and the change is used to quantify the analyte concentration. An alternate transistor configuration shown in Fig.…”

Section: Device Fabricationmentioning

confidence: 99%

Carbon nanomaterial-based electrochemical biosensors for label-free sensing of environmental pollutants

2016

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Carbon allotropes such as graphene and carbon nanotubes, have been incorporated in electrochemical biosensors for highly sensitive and selective detection of various analytes. The superior physical and electrical properties like high carrier mobility, ambipolar electric field effect, high surface area, flexibility and their compatibility with microfabrication techniques makes these carbon nanomaterials easy to integrate in field-effect transistor (FET)/chemiresistor type configuration which is suitable for portable and point-of-use/field-deployable sensors. This review covers the synthesis of carbon nanostructures (graphene and CNTs) and their integration into devices using various fabrication methods. Finally, we discuss the recent reports showing different sensing platforms that incorporate biomolecules like enzymes, antibodies and aptamers as recognition elements for fabrication of simple, low cost, compact biosensors that can be used for on-site, rapid environmental monitoring of environmental pollutants like pathogens, heavy metals, pesticides and explosives.

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Graphene- and aptamer-based electrochemical biosensor

Cited by 34 publications

References 27 publications

Detection of Immunoglobulin E with a Graphene-Based Field-Effect Transistor Aptasensor

Detection of Immunoglobulin E with a Graphene-Based Field-Effect Transistor Aptasensor

Bioelectronics and Interfaces Using Monolayer Graphene

Carbon nanomaterial-based electrochemical biosensors for label-free sensing of environmental pollutants

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