Grapevine Vitiviruses

Minafra, A.; Mawassi, Munir; Goszczynski, D. E.; Saldarelli, P.

doi:10.1007/978-3-319-57706-7_11

Cited by 38 publications

(19 citation statements)

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“…The impact of GVA on grafts and vascular tissue has been known for decades and it is considered to cause rugose wood complex in grapevines (Conti et al ). The sole impact of GVA on grapevines, however, has not yet been conclusively determined because it commonly occurs in mixed‐infections (Minafra et al ). According to our experience, it is rare to find single infections of grapevine vitiviruses in the field, and they are usually associated with GLRaVs.…”

Section: Discussionmentioning

confidence: 99%

Validation of micrografting to identify incompatible interactions of rootstocks with virus‐infected scions of Cabernet Franc

Cui

Agüero

Wang

et al. 2019

Australian Journal of Grape and Wine Research

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Background and Aims Grafted grapevines are a key component of modern viticulture. Grafting provides not only resistance to soil‐borne pests, but can also improve adaptability to the environment and can improve fruit yield and quality. Graft incompatibility induced by grapevine viruses causes damage to vineyards worldwide. The aim of the present study was to create a rapid and reliable means of evaluating graft incompatibility resulting from grafting virus‐infected scions onto healthy grape rootstocks. Methods and Results Scions of Control (healthy Cabernet Franc), LR131 (Cabernet Franc infected with GLRaV‐1) and LR132 (Cabernet Franc infected with GLRaV‐1 and GVA) were grafted onto different rootstocks by micrografting, green grafting and bench grafting methods. All the micrografts infected with LR132 failed, while LR131 and Control micrografts had similar survival rates. The impact of LR132 was less severe on green grafted plants, followed by bench grafts. Analysis of variance showed an interaction of grafting method × virus variety × rootstock, affecting grafting survival. When grafted onto Freedom and 101‐14, LR131 inhibited the growth of both scion and rootstock, but no significant impact was seen when LR131 was grafted onto St. George and AXR1. Well‐established callus was visible between the surfaces of LR131 and the rootstocks. The virus GLRaV‐1 was detected moving from the LR131 scion source to rootstocks 7 days after micrografting. Callus formation was delayed by LR132 between scion and rootstock surfaces, and a scion‐rootstock vascular connection did not establish, resulting in the death of all micrografts. Conclusions Micrografting is a reliable and sensitive method to screen for virus tolerant graft combinations compared with green and bench grafting. Significance of the Study The results of the present study validate micrografting as a means of producing credible information for selection of virus tolerant rootstocks, and ultimately vineyards with higher rates of compatible grafts.

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Section: Discussionmentioning

confidence: 99%

Validation of micrografting to identify incompatible interactions of rootstocks with virus‐infected scions of Cabernet Franc

Cui

Agüero

Wang

et al. 2019

Australian Journal of Grape and Wine Research

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“…No specific vector has been identified for Grapevine virus D (GVD). The role of ampeloviruses in the production of a help factor to assist in vitivirus transmission has not been confirmed (Minafra et al, 2017). GVA is the putative associated agent of Kober stem grooving, while GVB and GVD are reportedly involved in the aetiology of Corky bark syndrome, although results of studies investigating this involvement were not consistent.…”

Section: Rugose Wood Diseasementioning

confidence: 99%

“…As a result, the acidity levels of wine produced are altered, decreasing the commercial value of crops (Barba et al, 2015;Basso et al, 2014;Martelli, 2014;Nascimento et al, 2015). The four main, most widespread virus-associated disease complexes responsible for economic loss are Grapevine fleck disease, Grapevine fanleaf degeneration disease, Grapevine leafroll disease and Rugose wood disease (Minafra et al, 2017).…”

Section: Major Diseases Caused By Grapevine Virusesmentioning

confidence: 99%

Genetic diversity and identification of putative recombination events in grapevine rupestris stem pitting-associated virus

2018

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The impact of recombination on variant classification and the use of different genomic regions to identify virus variants were investigated using a diversity study performed on grapevine rupestris stem pitting-associated virus (GRSPaV). Three surveys were conducted to investigate the genetic diversity of GRSPaV and to compare the ability of the GRSPaV coat protein and replicase domains to classify virus variants. GRSPaV variants identified in the surveys clustered into five of the six currently recognised lineages, and a seventh, previously unclassified lineage was detected. A correlation was observed between the detection of recombinant GRSPaV sequences and inconsistencies in classification when using different genome regions for analysis.

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“…Vitiviruses appear to be latent in Vitis vinífera cultivars, and so far, only Grapevine virus A and Grapevine virus B had been consistently associated to grapevine diseases of the rugose wood complex (Grapevine vitiviruses) or Shiraz disease (reviewed by Minafra [10]). The etiological role of the recently described vitiviruses should be assessed in order to establish its relationships with known or unknown viral diseases.…”

Section: Reportmentioning

confidence: 99%

Grapevine virus L: a Novel Vitivirus in Grapevine

Debat

Zavallo²,

Brisbane³

et al. 2018

Preprint

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Vitivirus is a genus within the family Betaflexiviridae (Tymovirales) of ssRNA(+) viruses. There are currently ten ICTV recognized virus species in the genus; nevertheless, the extended use of NGS technologies is rapidly expanding their diversity and six more have been proposed recently. Here, we present the in silico characterization of a novel virus detected in grapevine (cv Riesling) which fits the genomic architecture and evolutionary constraints to be classifiable within the Vitivirus genus. The detected virus sequence is 7,607 nt long, including a typical genome organization of ORFs encoding a replicase (RP), a 22 kDa protein, a movement protein, a coat protein (CP) and a nucleic acid binding protein. Phylogenetic insights based on the predicted RP and CP, which share a 70.2% and 78.2% identity with its closest neighbour's grapevine virus E proteins, unequivocally clusters the identified virus within the Vitivirus genus. Multiple independent RNAseq data confirmed the presence of the detected virus in berries at diverse developmental stages. We tentatively propose the name grapevine virus L (GVL) to the detected putative Vitivirus. KeywordsVitivirus, Grapevine, cv Riesling, virus discovery, Betaflexiviridae Accession numberGrapevine virus L sequence (GVL-rs) has been deposited in GenBank under accession number MH248020. CC-BY-NC-ND 4.0 International license It is made available under a was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint (which . http://dx.doi.org/10.1101/314674 doi: bioRxiv preprint first posted online May. 4, 2018; ReportVitiviruses present flexuous, non-enveloped, filamentous virus particles of 725-785 nm and 12 nm length and diameter. Their nucleocapsid is cross-banded and striated diagonally. They have a linear ssRNA(+) genome (~7.3-7.6 kb), with a methylated nucleotide cap at the 5' end and a 3' poly (A) tail [1]. There are ten species of vitiviruses recognized by the ICTV, nevertheless, just in the last six months, six new species have been proposed, five of them infecting grapevine [2][3][4][5][6]. Grapevine is their most prevalent natural host but they have also been found to infect several crops such as Mint (Mentha x glaciaris), arracacha (Arracacia xanthorrhiza) blue agave (Agave tequilana) and kiwi (Actinidia chinense) [7][8][9].Vitiviruses appear to be latent in Vitis vinífera cultivars, and so far, only Grapevine virus A and Grapevine virus B had been consistently associated to grapevine diseases of the rugose wood complex (Grapevine vitiviruses) or Shiraz disease (reviewed by Minafra [10]). The etiological role of the recently described vitiviruses should be assessed in order to establish its relationships with known or unknown viral diseases. The synergistic effects between vitiviruses and other grapevine viruses appears to be significant [11]. The availability of complete sequences of these viruses could allow the development of full-length infectious clones, and ...

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Grapevine Vitiviruses

Cited by 38 publications

References 100 publications

Validation of micrografting to identify incompatible interactions of rootstocks with virus‐infected scions of Cabernet Franc

Validation of micrografting to identify incompatible interactions of rootstocks with virus‐infected scions of Cabernet Franc

Genetic diversity and identification of putative recombination events in grapevine rupestris stem pitting-associated virus

Grapevine virus L: a Novel Vitivirus in Grapevine

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