Grapevine MLO candidates required for powdery mildew pathogenicity?

Feechan, Angela; Jermakow, Angelica; Dry, Ian B.

doi:10.4161/psb.4.6.8575

Cited by 18 publications

(8 citation statements)

References 24 publications

(35 reference statements)

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“…The vesicle fusion events are controlled by MLO at the plasma membrane of host cells [12]. PM fungus may use MLO proteins as an 'invasion door' for the establishment of successful penetration before haustorium formation [34] by modulating plant antifungal defense reactions [13]. In addition, the TM domain and extracellular loops of CmMLO2, CmMLO3 and CmMLO13 contained evolutionarily conserved cysteine and proline residues, which could be related to susceptibility to PM fungus ( Fig.…”

Section: Discussionmentioning

confidence: 99%

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Expression Profiling of MLO Family Genes under Podosphaera xanthii Infection and Exogenous Application of Phytohormones in Cucumis melo L.

Howlader¹,

Kim²,

Park³

et al. 2016

Journal of Life Science

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Powdery mildew disease caused by Podosphaera xanthii is a major concern for Cucumis melo production worldwide. Knowledge on genetic behavior of the related genes and their modulating phytohormones often offer the most efficient approach to develop resistance against different diseases. Mildew Resistance Locus O (MLO) genes encode proteins with seven transmembrane domains that have significant function in plant resistance to powdery mildew fungus. We collected 14 MLO genes from 'Melonomics' database. Multiple sequence analysis of MLO proteins revealed the existence of both evolutionary conserved cysteine and proline residues. Moreover, natural genetic variation in conserved amino acids and their replacement by other amino acids are also observed. Real-time quantitative PCR expression analysis was conducted for the leaf samples of P. xanthii infected and phytohormones (methyl jasmonate and salicylic acid) treated plants in melon 'SCNU1154' line. Upon P. xanthii infection using 7 different races, the melon line showed variable disease reactions with respect to spread of infection symptoms and disease severity. Three out of 14 CmMLO genes were up-regulated and 7 were down-regulated in leaf samples in response to all races. The up-or down-regulation of the other 4 CmMLO genes was race-specific. The expression of 14 CmMLO genes under methyl jasmonate and salicylic acid application was also variable. Eleven CmMLO genes were up-regulated under salicylic acid treatment, and 7 were up-regulated under methyl jasmonate treatments in C. melo L. Taken together, these stress-responsive CmMLO genes might be useful resources for the development of powdery mildew disease resistant C. melo L.

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Section: Discussionmentioning

confidence: 99%

“…The medium size gene family MLO are variably distributed and 39, 15, 17, 12, 21, 13 and 38 MLO genes were noticed in soybean, Arabidopsis, grape, rice, apple, cucumber, and cotton, respectively [8,10,13,26,36,42,44]. MLO genes have been thoroughly studied both in many monocots and dicots.…”

Section: Introductionmentioning

confidence: 99%

Expression Profiling of MLO Family Genes under Podosphaera xanthii Infection and Exogenous Application of Phytohormones in Cucumis melo L.

Howlader¹,

Kim²,

Park³

et al. 2016

Journal of Life Science

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“…tomato and Xanthomonas campestris pv. vesicatoria [ 60,61 ]; wounding [ 21,37,62 ]; phytohormone [ 24,26,63 ] and abiotic stresses [ 63,64 ]. As a rule, however, the paralogs in MLO family, as illuminated by their clustering pattern (Fig.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide Identiﬁcation, Classiﬁcation and Expression Analysis of the Mildew Resistance Locus O ( MLO ) Gene Family in Sweet Orange ( Citrus sinensis )

Liu

JinWang

et al. 2017

Braz. arch. biol. technol.

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“…Moreover, on the footprint of research conducted on model plants, genes associated with mildew susceptibility have been discovered and dissected also in the grapevine genome. 17 VvMLO genes, orthologues of the Arabidopsis MLOs , were identified and a few members showed transcriptional induction upon fungal inoculation [ 65 , 66 ]. Lately, more insights in terms of powdery mildew resistance has been achieved by silencing of four VvMLO genes through RNAi in grapevine [ 67 ].…”

Section: Introductionmentioning

confidence: 99%

Mining Grapevine Downy Mildew Susceptibility Genes: A Resource for Genomics-Based Breeding and Tailored Gene Editing

Pirrello

Zeilmaker²,

Bianco

et al. 2021

Biomolecules

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Several pathogens continuously threaten viticulture worldwide. Until now, the investigation on resistance loci has been the main trend to understand the interaction between grapevine and the mildew causal agents. Dominantly inherited gene-based resistance has shown to be race-specific in some cases, to confer partial immunity, and to be potentially overcome within a few years since its introgression. Recently, on the footprint of research conducted in Arabidopsis, putative genes associated with downy mildew susceptibility have been discovered also in the grapevine genome. In this work, we deep-sequenced four putative susceptibility genes—namely VvDMR6.1, VvDMR6.2, VvDLO1, VvDLO2—in 190 genetically diverse grapevine genotypes to discover new sources of broad-spectrum and recessively inherited resistance. Identified Single Nucleotide Polymorphisms were screened in a bottleneck analysis from the genetic sequence to their impact on protein structure. Fifty-five genotypes showed at least one impacting mutation in one or more of the scouted genes. Haplotypes were inferred for each gene and two of them at the VvDMR6.2 gene were found significantly more represented in downy mildew resistant genotypes. The current results provide a resource for grapevine and plant genetics and could corroborate genomic-assisted breeding programs as well as tailored gene editing approaches for resistance to biotic stresses.

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Grapevine MLO candidates required for powdery mildew pathogenicity?

Cited by 18 publications

References 24 publications

Expression Profiling of MLO Family Genes under Podosphaera xanthii Infection and Exogenous Application of Phytohormones in Cucumis melo L.

Expression Profiling of MLO Family Genes under Podosphaera xanthii Infection and Exogenous Application of Phytohormones in Cucumis melo L.

Genome-wide Identiﬁcation, Classiﬁcation and Expression Analysis of the Mildew Resistance Locus O ( MLO ) Gene Family in Sweet Orange ( Citrus sinensis )

Mining Grapevine Downy Mildew Susceptibility Genes: A Resource for Genomics-Based Breeding and Tailored Gene Editing

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