Grapevine Crown Gall Suppression Using Biological Control and Genetic Engineering: A Review of Recent Research

Filo, Attila; Sabbatini, Paolo; Sundin, George W.; Zabadal, Thomas J.; Safe, Gene R.; Cousins, Peter

doi:10.5344/ajev.2012.12038

Cited by 19 publications

(10 citation statements)

References 96 publications

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“…In addition to A. vitis, other virulent Agrobacterium species are known to induce grapevine crown gall development (4). A. vitis is known to persist in debris from infested grapevine material in soil (5) and can enter grapevines via the root and move through the xylem (6) to wounded parts of the plant, where it transforms the cells (7)(8)(9). The pathogen has been detected in the xylem sap of canes, so propagation material of grapevine nurseries serves as an additional risk for distributing A. vitis (10,11).…”

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confidence: 99%

Grapevine (Vitis vinifera) Crown Galls Host Distinct Microbiota

Faist¹,

Keller

Hentschel³

et al. 2016

Appl Environ Microbiol

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Crown gall disease of grapevine is caused by virulent Agrobacterium strains and establishes a suitable habitat for agrobacteria and, potentially, other bacteria. The microbial community associated with grapevine plants has not been investigated with respect to this disease, which frequently results in monetary losses. This study compares the endophytic microbiota of organs from grapevine plants with or without crown gall disease and the surrounding vineyard soil over the growing seasons of 1 year. Amplicon-based community profiling revealed that the dominating factor causing differences between the grapevine microbiota is the sample site, not the crown gall disease. The soil showed the highest microbial diversity, which decreased with the distance from the soil over the root and the graft union of the trunk to the cane. Only the graft union microbiota was significantly affected by crown gall disease. The bacterial community of graft unions without a crown gall hosted transient microbiota, with the three most abundant bacterial species changing from season to season. In contrast, graft unions with a crown gall had a higher species richness, which in every season was dominated by the same three bacteria (Pseudomonas sp., Enterobacteriaceae sp., and Agrobacterium vitis). For in vitro-cultivated grapevine plantlets, A. vitis infection alone was sufficient to cause crown gall disease. Our data show that microbiota in crown galls is more stable over time than microbiota in healthy graft unions and that the microbial community is not essential for crown gall disease outbreak. IMPORTANCEThe characterization of bacterial populations in animal and human diseases using high-throughput deep-sequencing technologies, such as 16S amplicon sequencing, will ideally result in the identification of disease-specific microbiota. We analyzed the microbiota of the crown gall disease of grapevine, which is caused by infection with the bacterial pathogen Agrobacterium vitis. All other Agrobacterium species were found to be avirulent, even though they lived together with A. vitis in the same crown gall tumor. As has been reported for human cancer, the crown gall tumor also hosted opportunistic bacteria that are adapted to the tumor microenvironment. Characterization of the microbiota in various diseases using amplicon sequencing may help in early diagnosis, to serve as a preventative measure of disease in the future.

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confidence: 99%

Grapevine (Vitis vinifera) Crown Galls Host Distinct Microbiota

Faist¹,

Keller

Hentschel³

et al. 2016

Appl Environ Microbiol

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“…Bacterial genera studied for the control of crown gall disease include Pseudomonas, Bacillus, Serratia and Agrobaterium. The most successfully used antagonist strain is A. radiobacter K84 [6][7][8][9][10]. This strain was isolated in Australia, and it has an inhibitory action on most pathogenic A. tumefaciens containing the nopaline Ti plasmid (biotype Ⅰand Ⅱ), although some strains of A. tumefaciens biotype Ⅰ and Ⅱ became resistant to K84 agrocin [11].…”

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confidence: 99%

Identification and Characterization of a Pseudomonas mosselii strain and its Antibacterial Function against Agrobacterium tumefaciens

Wang¹,

Li²,

Wang³

et al. 2021

SDRP JPS

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Agrobacterium tumefaciens is a gram negative bacterial that can infect a range of plants and result in root crown gall. A total number of 10328 bacterial strains were isolated from rhizosphere of cherry tree. One strain of LWB10 showed clear inhibition zone around the bacterial colony in YEB media inoculated with A. tumefaciens C58. Morphological, physiological, and biochemical characterization indicated that LWB10 belongs to member of the genus Pseudomonas. Results from the high-throughput matrix-assisted laser desorption/ionization biotypersmart system indicated that this strain had a score value of 2.247 relative to Pseudomonas mosselii. Also, phylogenetic analysis based on 16S rRNA gene sequence showed that strain LWB10 shared the highest similarity with Pseudomonas mosselii CIP 105259T. The antagonist strains also exhibit well in growth inhibition of other five A. tumefaciens strains. Coinoculation of LWB10 and plant pathogenic strain of A. tumefaciens CFCC1369 showed strongly inhibition of tumor formation in tomato stems. All the results demonstrated that the isolated strain is P. mosselii LWB10 and its antibacterial ability to A. tumefaciens may offer new way for management of crown gall disease in the future. Keywords: Agrobacterium tumefaciens, Pseudomonas mosselii, antagonist, biological control

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“…Others include a Bacillus subtilis treatment on peanuts to reduce root canker and increase yield (Turner and Backman, 1991), and fluorescent Pseudomonads to promote plant growth to protect various crops, such as potato, sugarbeet and wheat (Haas and Defago, 2005). In grapevines, nontumorigenic strains of Agrobacterium vitis are being developed to suppress crown gall formation (Burr and Reed, 1994;Filo et al, 2013;Kawaguchi et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Xylella fastidiosa PD1311 gene mutant and its suppression of Pierce's disease on grapevines

Hao

Johnson

Cursino

et al. 2016

Molecular Plant Pathology

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Xylella fastidiosa causes Pierce's disease (PD) on grapevines, leading to significant economic losses in grape and wine production. To further our understanding of X. fastidiosa virulence on grapevines, we examined the PD1311 gene, which encodes a putative acyl-coenzyme A (acyl-CoA) synthetase, and is highly conserved across Xylella species. It was determined that PD1311 is required for virulence, as the deletion mutant, ΔPD1311, was unable to cause disease on grapevines. The ΔPD1311 strain was impaired in behaviours known to be associated with PD development, including motility, aggregation and biofilm formation. ΔPD1311 also expressed enhanced sensitivity to H O and polymyxin B, and showed reduced survival in grapevine sap, when compared with wild-type X. fastidiosa Temecula 1 (TM1). Following inoculation, ΔPD1311 could not be detected in grape shoots, which may be related to its altered growth and sensitivity phenotypes. Inoculation with ΔPD1311 2 weeks prior to TM1 prevented the development of PD in a significant fraction of vines and eliminated detectable levels of TM1. In contrast, vines inoculated simultaneously with TM1 and ΔPD1311 developed disease at the same level as TM1 alone. In these vines, TM1 populations were distributed similarly to populations in TM1-only inoculated plants. These findings suggest that, through an indirect mechanism, pretreatment of vines with ΔPD1311 suppresses pathogen population and disease.

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Grapevine Crown Gall Suppression Using Biological Control and Genetic Engineering: A Review of Recent Research

Cited by 19 publications

References 96 publications

Grapevine (Vitis vinifera) Crown Galls Host Distinct Microbiota

Grapevine (Vitis vinifera) Crown Galls Host Distinct Microbiota

Identification and Characterization of a Pseudomonas mosselii strain and its Antibacterial Function against Agrobacterium tumefaciens

Characterization of the Xylella fastidiosa PD1311 gene mutant and its suppression of Pierce's disease on grapevines

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