Grape Seed Proanthocyanidin Extract Ameliorates Cardiac Remodelling After Myocardial Infarction Through PI3K/AKT Pathway in Mice

Ruan, Yongxue; Jin, Qike; Zeng, Jingjing; Ren, Fangfang; Xie, Zuo-yi; Ji, Kangting; Wu, Lianpin; Wu, Jingguo; Li, Lei

doi:10.3389/fphar.2020.585984

Cited by 23 publications

(18 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, GSPE was reported to induce apoptosis in the CaCo2 colorectal cancer cell line via a mechanism associated with the attenuation of the PI3K/PKB pathway ( 21 ). However, Ruan et al ( 32 ) reported that GSPE is associated with decreased Bax expression and increased Bcl-2 expression, thus playing a critical role in the inhibition of H9C2 cell apoptosis after hypoxic culturing via the PI3K/AKT signaling pathway. Further beneficial effects exerted by GSPE include the prevention of bone loss induced by inflammation via a decrease in the phosphorylation and degradation of NF-κB inhibitor and inhibition of apoptosis via a decrease in the activity of caspases-3 and -9 ( 33 ).…”

Section: Discussionmentioning

confidence: 99%

Grape seed proanthocyanidin extract induces apoptosis of HL-60/ADR cells via the Bax/Bcl-2 caspase-3/9 signaling pathway

Lin¹,

Zhao²,

Huang³

et al. 2021

Transl Cancer Res TCR

View full text Add to dashboard Cite

Background Our previous study detailed the direct induction of apoptosis by grape seed proanthocyanidin extract (GSPE) in a multidrug resistant human acute myeloid leukemia (AML) HL-60/adriamycin (HL-60/ADR) cell line, although the mechanism of this effect was not detailed. This study aims to elucidate the mechanism underlying GSPE-induced cell apoptosis in HL-60/ADR cells. Methods HL-60/ADR cells were studied to evaluate effects of GSPE (0–100 µg/mL); a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was employed to identify the cytotoxic effect of varying GSPE concentrations. Trypan blue staining was used to observe changes in cell viability; flow cytometry assays were used to verify apoptosis. Expression of Bax and Bcl-2 mRNA was analyzed using real-time polymerase chain reaction (PCR). Activity of caspase-3 and caspase-9 was also detected. Results Here, GSPE was found to inhibit HL-60/ADR cell growth and induce cell apoptosis in a dose-dependent manner. Real-time PCR findings revealed that GSPE concentrations above 75 µg/mL significantly increase expression of Bax mRNA (P<0.001). GSPE concentrations above 25 µg/mL were found to significantly decrease expression of Bcl-2 mRNA (P<0.01), while concentrations above 50 µg/mL were found to significantly increase caspase-3 activity after 6, 12 and 24 h (P<0.01). However, only 100 µg/mL GSPE was found to significantly increase caspase-9 activity (P<0.001 at 6 and 12 h; P<0.05 at 24 h). Conclusions GSPE inhibits the proliferation of HL-60/ADR cells by the induction of apoptosis in a dose-dependent manner via the Bax/Bcl-2 caspase-3/9 signaling pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

Grape seed proanthocyanidin extract induces apoptosis of HL-60/ADR cells via the Bax/Bcl-2 caspase-3/9 signaling pathway

Lin¹,

Zhao²,

Huang³

et al. 2021

Transl Cancer Res TCR

View full text Add to dashboard Cite

show abstract

“…The bioactive grape compounds additionally improve appearance, health, and protective skin function, by acting locally as an anti-cellulite agent, improving circulation, preventing and reducing skin hyperpigmentation (freckles, age pigment, and melasma), collagen degradation and denaturation, etc. Furthermore, the influence of antioxidant defense at all levels (cell, tissue organs, system of organs) has also been studied concerning peristalsis in the digestive tract [15,16], myocardial infarction [17,18], and reproductive organs [19,20]. In other words, there is no cell, tissue or organ that does not enjoy the beneficial effects of natural nontoxic unique super-cocktails of grape bioactive substances.…”

Section: Introductionmentioning

confidence: 99%

Redox properties of grape wine skin extracts from the Šumadija region: An electron paramagnetic resonance study

Nakarada¹,

Marković²,

Popović³

et al. 2021

Hosp Pharm Int Multi J

View full text Add to dashboard Cite

Introduction: Comprising of a unique combination of bioactive polyphenol compounds, grapes are shown to have a beneficial effect on the skin as well as the whole body. They exhibit high antioxidative and antiradical activity through mechanisms of heavy metals chelation, scavenging reactive oxygen species, inhibiting lipid peroxidation, and preserving the integrity of the cell membranes. In the winemaking industry, after crushing and pressing, grape pomace (stems, seeds, pulp, and skin) is removed as a by-product. This valuable source of bioactive compounds is often treated as animal food, compost, raw material in the brewery, but unfortunately mostly disposed of as unusable waste. Aim: The aim of this study was to compare redox properties of absolute EtOH, 50% EtOH, and H2 O grape wine skin extracts in a way of scavenging DPPH radicals, showing that biowaste obtained from grape wineskins could be used as an immense source of bioactive compounds with high antiradical activity. Material and Methods: Electron Paramagnetic Resonance (EPR) spectroscopy was used to detect the activity of grape skin extracts toward DPPH radicals and UHPLC-DAD MS/MS analysis to separate, identify and quantify their active components. Results: The results show that Cabernet Sauvignon and Pinot Blanc grape skin extracts have unexpectedly large potential to remove DPPH radicals from the system. Having in mind the concentration of redox-active components obtained by UHPLC-DAD MS/MS analysis and presented EPR results, there is strong evidence that primarily quercetin 3-O-glucoside, as well as kaempferol 3-O-glucoside, isorhamnetin 3-O-glucoside, kaempferol 7-O-glucoside supplemented with catechin and rutin are responsible for the antioxidative capacity of extracts. Conclusions: This study demonstrates that extracts obtained from grape skins, initially intended as biowaste, exhibit high antiradical activity. The largest quantity of the most redox-active components could be found in 50% EtOH extracts, which we propose to be the starting material for making potent redox-active cosmetic products and dietary supplements.

show abstract

“…Although there are other related genes, including VEGF-B and VEGF-C, VEGF-A plays a dominant role in regulating angiogenesis (Apte et al 2019 ). PI3K/Akt signaling plays an important role in angiogenesis and exerts a protective role in myocardial infarction (Peng et al 2021 ; Ruan et al 2020 ). Bertrand et al .…”

Section: Discussionmentioning

confidence: 99%

Gastrin exerts a protective effect against myocardial infarction via promoting angiogenesis

Tang

Zhang

et al. 2021

Mol Med

View full text Add to dashboard Cite

Background It is known that increased gastrin concentration is negatively correlated with cardiovascular mortality, and plasma gastrin levels are increased in patients after myocardial infarction (MI). However, whether gastrin can play a protective role in MI remains unknown. Methods Adult C57BL/6 mice were subjected to ligation of the left anterior descending coronary artery (LAD) and subcutaneous infusion of gastrin (120 μg/Kg body weight/day, 100 μL in the pump) for 28 days after MI. Plasma gastrin concentrations were measured through an ELISA detection kit. Mice were analyzed by echocardiography after surgery. CD31 and VEGF expression were quantified using immunofluorescence staining or/and western blot to assess the angiogenesis in peri-infarct myocardium. Capillary-like tube formation and cell migration assays were performed to detect gastrin-induced angiogenesis. Results We found that gastrin administration significantly ameliorated MI-induced cardiac dysfunction and reduced fibrosis at 28 days in post-MI hearts. Additionally, gastrin treatment significantly decreased cardiomyocyte apoptosis and increased angiogenesis in the infarct border zone without influencing cardiomyocyte proliferation. In vitro results revealed that gastrin up-regulated the PI3K/Akt/vascular endothelial growth factor (VEGF) signaling pathway and promoted migration and tube formation of human coronary artery endothelial cells (HCAECs). Cholecystokinin 2 receptor (CCK2R) mediated the protective effect of gastrin since the CCK2R blocker CI988 attenuated the gastrin-mediated angiogenesis and cardiac function protection. Conclusion Our data revealed that gastrin promoted angiogenesis and improved cardiac function in post-MI mice, highlighting its potential as a therapeutic target candidate.

show abstract

Grape Seed Proanthocyanidin Extract Ameliorates Cardiac Remodelling After Myocardial Infarction Through PI3K/AKT Pathway in Mice

Cited by 23 publications

References 46 publications

Grape seed proanthocyanidin extract induces apoptosis of HL-60/ADR cells via the Bax/Bcl-2 caspase-3/9 signaling pathway

Grape seed proanthocyanidin extract induces apoptosis of HL-60/ADR cells via the Bax/Bcl-2 caspase-3/9 signaling pathway

Redox properties of grape wine skin extracts from the Šumadija region: An electron paramagnetic resonance study

Gastrin exerts a protective effect against myocardial infarction via promoting angiogenesis

Contact Info

Product

Resources

About