Grape seed pro‐anthocyanidins ameliorates radiation‐induced lung injury

Huang, Yijuan; Li, Wen; Liu, Hu; Yang, Yue; Cui, Jianguo; Zhang, Pei; Zhao, Hainan; He, Feng; Cheng, Ying; Jin, Ning; Cai, Jianming; Li, Bailong; Fu, Guosheng

doi:10.1111/jcmm.12276

Cited by 22 publications

(27 citation statements)

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“…The fact that antioxidants were effective in ameliorating ionizing radiation-induced oxidative injuries has been demonstrated [ 12 , 13 ]. Previously, we have assessed that GSPs exhibited significant free radical scavenging ability in a cell-free Fenton system [ 4 ]. In the current study, we demonstrated that GSPs protected AHH-1 cells and mice from IR-induced injury, which may result from their radical scavenging effect.…”

Section: Discussionmentioning

confidence: 99%

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Radioprotective Effect of Grape Seed Proanthocyanidins In Vitro and In Vivo

Huang

Zhao

Cao

et al. 2016

Oxidative Medicine and Cellular Longevity

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We have demonstrated that grape seed proanthocyanidins (GSPs) could effectively scavenge hydroxyl radical (•OH) in a dose-dependent manner. Since most of the ionizing radiation- (IR-) induced injuries were caused by •OH, this study was to investigate whether GSPs would mitigate IR-induced injuries in vitro and in vivo. We demonstrated that GSPs could significantly reduce IR-induced DNA strand breaks (DSBs) and apoptosis of human lymphocyte AHH-1 cells. This study also showed that GSPs could protect white blood cells (WBC) from IR-induced injuries, speed up the weight of mice back, and decrease plasma malondialdehyde (MDA), thus improving the survival rates of mice after ionizing radiation. It is suggested that GSPs have a potential as an effective and safe radioprotective agent.

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Section: Discussionmentioning

confidence: 99%

“…Previously, we have demonstrated that grape seed proanthocyanidins (GSPs) could effectively scavenge hydroxyl radical (•OH) in a dose-dependent manner [ 4 ]. In the current study, we investigated whether GSPs exerted radioprotective effect in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

Radioprotective Effect of Grape Seed Proanthocyanidins In Vitro and In Vivo

Huang

Zhao

Cao

et al. 2016

Oxidative Medicine and Cellular Longevity

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“…There were two waves of increase in IFN-γ (same trend as for TGF-β1). IL-4 and IL-13 levels of mRNA in the lungs remained lower in the early time points, but stepped up progressively over 4 w. Huang et al [ 167 ] IL-4, IFN-γ, IL-12 C57Bl/6 Thorax 60 Co/20 Gy 1, 3 and 6 mo Invitrogen Bioplex BAFL The fibrotic cytokine IL-4 level in BALF from mice in the irradiation group increased significantly, the IL-12 level in BALF increased 1 mo after irradiation, and the IFN-γ level in BALF decreased significantly at intervals 3 and 6 mo. Xu et al [ 168 ] TNF-α, IL-17A, TGF-β1, IL-6 C57Bl/6 Thorax X-ray/15 Gy 1, 4, 8 and 16 w ELISA BALF The levels of TNF-α, IL-6 and TGF-β1 were significantly higher after irradiation at the 4 w time point; IL-17A reached a maximal concentration at 4 w, then slightly decreased.…”

Section: Cytokine Responses To Radiationmentioning

confidence: 99%

Cytokines and radiation-induced pulmonary injuries

Lierová

Jeličová

Němcová

et al. 2018

Journal of Radiation Research

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Radiation therapy is one of the most common treatment strategies for thorax malignancies. One of the considerable limitations of this therapy is its toxicity to normal tissue. The lung is the major dose-limiting organ for radiotherapy. That is because ionizing radiation produces reactive oxygen species that induce lesions, and not only is tumor tissue damaged, but overwhelming inflammatory lung damage can occur in the alveolar epithelium and capillary endothelium. This damage may result in radiation-induced pneumonitis and/or fibrosis. While describing the lung response to irradiation generally, the main focus of this review is on cytokines and their roles and functions within the individual stages. We discuss the relationship between radiation and cytokines and their direct and indirect effects on the formation and development of radiation injuries. Although this topic has been intensively studied and discussed for years, we still do not completely understand the roles of cytokines. Experimental data on cytokine involvement are fragmented across a large number of experimental studies; hence, the need for this review of the current knowledge. Cytokines are considered not only as molecular factors involved in the signaling network in pathological processes, but also for their diagnostic potential. A concentrated effort has been made to identify the significant immune system proteins showing positive correlation between serum levels and tissue damages. Elucidating the correlations between the extent and nature of radiation-induced pulmonary injuries and the levels of one or more key cytokines that initiate and control those damages may improve the efficacy of radiotherapy in cancer treatment and ultimately the well-being of patients.

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“…Lungs of mice received irradiation at a total dose of 20 Gy and a dose rate of 1 Gy/min. The irradiation source was 60 Co from the radiation center (Faculty of Naval Medicine, Second Military Medical University, China) [21, 22]. …”

Section: Methodsmentioning

confidence: 99%

Hypoxia-Induced Mesenchymal Stromal Cells Exhibit an Enhanced Therapeutic Effect on Radiation-Induced Lung Injury in Mice due to an Increased Proliferation Potential and Enhanced Antioxidant Ability

Zhu

et al. 2017

Cell Physiol Biochem

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Background/Aims: Radiation therapy is an important treatment for thoracic cancer; however, side effects accompanied with radiotherapy lead to limited tumor control and a decline in patient quality of life. Among these side effects, radiation-induced lung injury (RILI) is the most serious and common. Hence, an effective remedy for RILI is needed. Mesenchymal stromal cells (MSCs) are multipotent adult stem cells that have been demonstrated to be an effective treatment in some disease caused by tissue damage. However, unlike other injuries, RILI received limited therapeutic effects from implanted MSCs due to local hypoxia and extensive reactive oxygen species (ROS) in irradiated lungs. Since the poor survival of MSCs is primarily due to hypoxia and ROS generation, we hypothesize that persistent and adaptive hypoxia treatment induces enhanced resistance to hypoxic stress in implanted MSC. The aim of this study is to investigate whether persistent and adaptive hypoxia treatment of bmMSCs prior to their transplantation in injured mice enhanced survival and improved curative effects in RILI. Methods: Primary bmMSCs were obtained from the marrow of six-week-old male C57BL6/J mice and were cultured either under normoxic conditions (21% O₂) or hypoxic conditions (2.5% O₂). Mice were injected with normoxia/hypoxia MSCs after thoracic irradiation (20 Gy). The therapeutic effects of MSCs on RILI were assessed by pathological examinations that included H&E staining, Masson staining and α-SMA staining; meanwhile, inflammatory factors were measured using an ELISA. The morphology of MSCs in vitro was recorded using a microscope and identified by flow cytometry, cell viability was measured using the CCK-8 assay, the potential for proliferation was detected by the EdU assay, and ROS levels were measured using a ROS fluorogenic probe. In addition, HIF-1α and several survival pathway proteins (Akt, p-Akt, Caspase-3) were also detected by western blotting. Results: Implanted MSCs alleviated both early radiation-induced pneumonia and late pulmonary fibrosis. However, hypoxia MSCs displayed a more pronounced therapeutic effect compared to normoxia MSCs. Compared to normoxia MSCs, the hypoxia MSCs demonstrated greater cell viability, an enhanced proliferation potential, decreased ROS levels and increased resistance to hypoxia and ROS stress. In addition, hypoxia MSCs achieved higher activation levels of HIF-1α and Akt, and HIF-1α played a critical role in the development of resistance. Conclusion: Hypoxia enhances the therapeutic effect of mesenchymal stromal cells on radiation-induced lung injury by promoting MSC proliferation and improving their antioxidant ability, mediated by HIF-1α.

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Grape seed pro‐anthocyanidins ameliorates radiation‐induced lung injury

Cited by 22 publications

References 50 publications

Radioprotective Effect of Grape Seed Proanthocyanidins In Vitro and In Vivo

Radioprotective Effect of Grape Seed Proanthocyanidins In Vitro and In Vivo

Cytokines and radiation-induced pulmonary injuries

Hypoxia-Induced Mesenchymal Stromal Cells Exhibit an Enhanced Therapeutic Effect on Radiation-Induced Lung Injury in Mice due to an Increased Proliferation Potential and Enhanced Antioxidant Ability

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