Grape seed extract and Zinc containing nutritional food supplement delays onset and progression of Streptozocin-induced diabetic cataract in Wistar rats

Abstract

Prevention of hyperglycemia and enhancement of antioxidant defense mechanisms remain major goals in the treatment of diabetic cataract. Earlier, we reported strong antihyperglycemic and in vitro antioxidant potential of the combined formulation of grape seed extract and Zincovit tablets. Therefore, the current study was designed to investigate effects of combined formulation of grape seed extract and Zincovit tablets against streptozocin-induced diabetic cataract in Wistar rats. Adult Wistar rats were selected and diabetes was induced by streptozocin (35 mg/kg, i.p) and divided into four groups (group II-V). The normal control (group I) and streptozocin-induced diabetic cataract control rats received only vehicle. Groups III, IV and V animals received orally 40, 80 and 160 mg/kg of combined formulation of Zincovit tablets with grape seed extract respectively for a period of 150 days. The biochemical pathways involved in the pathogenesis of cataract such as oxidative stress, polyol pathway and alterations in adenosine triphosphate, glucose-6-phosphate dehydrogenase and blood glucose were investigated, to understand the possible mechanism of action of combined formulation of grape seed extract and Zincovit tablets. Rats treated with combined formulation of grape seed extract and Zincovit tablets delayed the progression of diabetic cataract as well as it showed significant alterations in oxidative stress markers along with blood glucose, aldose reductase, glucose-6-phosphate dehydrogenase and adenosine triphosphate level in lens. Over all, the results suggest that single combined formulation of grape seed extract and Zincovit tablets may be of great value in delaying diabetic cataract of human subjects as nutritional food supplement.

Protective effects of Vitamin E on CCl4-induced testicular toxicity in male rats

“…When this defense mechanism is not complete or when ROS are excessively generated, additional protecting mechanisms of dietary anti-oxidants could be of a great significance. This explains the increased number of the proposed natural and synthetic anti-oxidative agents that treat reproductive hormonal imbalance and infertility induced by oxidative stress (35). Furthermore, recent results suggested that natural products containing anti-oxidants have the ability to protect testis against oxidative damages, lipid peroxidation, and impairment in anti-oxidant status induced by CCl 4 (3,13,35).…”

Influence of Grape Seed Extract and Zinc Containing Multivitamin-Mineral Nutritional Food Supplement on Lipid Profile in Normal and Diet-Induced Hypercholesterolemic Rats

“…One of the studies suggests that flavonoids possess antioxidant properties and have been shown to be potent inhibitors of LDL oxidation in vitro [9]. We had reported combined formulation of grape seed extract and Zincovit tablets for its strong in vitro, in vivo antioxidant, anti-hyperglycemic and anti-cataractogenic potential [10][11][12][13][14]. Consequently, the aim of the present study was to investigate the influence of single combined formulation of grape seed extract and Zincovit tablets (Nutritional food supplement) on lipid profile in normal and diet-induced hypercholesterolemic rats.…”

“…Earlier, we had reported combined formulation of grape seed extract and Zincovit tablets for its strong in vitro, in vivo antioxidant, anti-hyperglycemic and anti-cataractogenic potential [10][11][12][13][14]. A recent study has shown that a combination of IH636 grape seed proanthocyanidin extract and niacin-bound chromium can decrease total cholesterol, LDL and oxidized LDL level in hypercholesterolemic human subjects [9].…”

Zincovit syrup ameliorates oxidative stress induced by carbon tetrachloride in rats

“…The rats were divided into six groups containing six rats in each group. Treatment was done for 7 days as follow: 6 Group I: Normal control rats were given 2% gum acacia (1 ml/kg/day; p.o). Group II: CCl 4 intoxicated control rats + 2% gum acacia (1 ml/kg/day; p.o) and simultaneously administered At the end of 7 th day treatment, all the experimental rats were kept overnight fasting and sacrificed by administering an overdose of ketamine, i.p.…”

“…Earlier, we had reported the antioxidant potential of combined formulation of grape seed extract and ZVT tablets. 5,6 Consequently, the aim of the present study was to investigate the antioxidant potential of ZVT syrup against CCl 4 intoxicated Wistar rats.…”