Grape Pomace Extract Attenuates Inflammatory Response in Intestinal Epithelial and Endothelial Cells: Potential Health-Promoting Properties in Bowel Inflammation

Calabriso, Nadia; Massaro, Marika; Scoditti, Egeria; Verri, Tiziano; Barca, Amilcare; Gerardi, C.; Giovinazzo, Giovanna; Carluccio, Maria Annunziata

doi:10.3390/nu14061175

Cited by 27 publications

(23 citation statements)

References 85 publications

(121 reference statements)

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Section: Health Benefitsmentioning

confidence: 99%

“…Besides gut microbiota, GP benefits were already tested, by Calabriso et al [ 96 ], for their potential to diminish the overwhelming inflammatory response in enterocyte-like cells. They carried out an in vitro study with intestinal epithelial Caco-2 cells, grown in monolayers or in co-culture with endothelial cells (Caco-2/HMEC-1) [ 96 ]. Cultures were treated for two hours with different concentrations of GP (1, 5, 10 µg/mL gallic acid equivalents) and then stimulated, for 16 h, with lipopolysaccharide (LPS) and tumor necrosis factor (TNF).…”

Section: Health Benefitsmentioning

confidence: 99%

“…Regarding endothelial cells, the GP supplementation decreased the endothelial expression of IL-6, MCP-1, VCAM-1, and ICAM-1. Because of that, GP inhibited the adhesion of leukocytes to the endothelial cells under pro-inflammatory conditions [ 96 ]. This ability of GP to modulate various pathways involved in inflammation and oxidative stress observed in vitro could help explain the protection previously observed in rats.…”

Section: Health Benefitsmentioning

confidence: 99%

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The Wine Industry By-Products: Applications for Food Industry and Health Benefits

Ferrer‐Gallego

Silva

2022

Antioxidants

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Each year, 20 million tons of wine by-products are generated, corresponding to 30% of the total quantity of vinified grapes. Wine by-products are a source of healthy bioactive molecules, such as polyphenols and other molecules (pigments, fibers, minerals, etc.). The abundance of bioactive compounds assures a promising future for nutritional foodstuff production. Wine by-products can be used to fortify aromatized waters and infusions, bread, pasta, dairy products, alcohol, sugary beverages, and processed foods. These innovative products are part of the Mediterranean diet and are of great interest to both human and environmental health. Pre-clinical studies show that consumption of food produced with wine by-products or with their extracts attenuates the inflammatory state and increases antioxidant status. As such, wine by-products provide protective effects against the underlying pathophysiological hallmarks of some chronic diseases such as atherosclerosis, diabetes, hypertension, obesity, and cancer. However, the poor bioavailability warrants further investigation on how to optimize the efficacy of wine by-products, and more clinical trials are also needed. The scientific evidence has validated the uses of the dietary nature of wine by-products and has helped to promote their use as a functional food to prevent chronic human diseases.

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Section: Health Benefitsmentioning

confidence: 99%

Section: Health Benefitsmentioning

confidence: 99%

Section: Health Benefitsmentioning

confidence: 99%

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The Wine Industry By-Products: Applications for Food Industry and Health Benefits

Ferrer‐Gallego

Silva

2022

Antioxidants

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“…In the study of Calabriso et al, (2022) the anti-inflammatory effects of RGP from Vitis vinifera L., cv Negramaro from Guagnano, Lecce, Italy was investigated on LPS and TNF-α-induced inflammation in Caco-2 cells and in human microvascular endothelial cells (HMEC-1 cells). Using the ELISA assay, they observed that the treatment with RGP induced a decrease in IL-6 (interleukin-6) and MCP-1 (monocyte chemoattractant protein-1) levels and down-regulation of MMP-9 (matrix metalloproteinases-9) and MMP-2 (matrix metalloproteinases-2) expression [ 45 ]. Moreover, they also highlighted the down-regulation of the NF- κB pro-inflammatory signaling pathways and the down-regulation of the pro-inflammatory proteins such as cytokines (IL-1β and TNF-α) and chemokines.…”

Section: Red and White Grape Pomace—in Vitro Antioxidant And Anti-inf...mentioning

confidence: 99%

Antioxidant and Anti-Inflammatory Actions of Polyphenols from Red and White Grape Pomace in Ischemic Heart Diseases

et al. 2022

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Grape pomace (GP) represents a very reliable source of polyphenols because it could be found globally as a remnant of the wine industry. During the winemaking process, two types of GP are generated: red GP and white GP, according to the produced wine, red or white. Grape pomace represents a viable source of polyphenols, mainly flavanols, procyanidins anthocyanins, and resveratrol which possess antioxidant and anti-inflammatory activities. Multiple differences were observed between red and white GP in terms of their antioxidant and anti-inflammatory activity in both in vitro and in vivo studies. Although most studies are focused on the antioxidant and anti-inflammatory effect of red grape pomace, there are still many variables that need to be taken into consideration, as well as extensive study of the white GP. It was observed that in both in vitro and in vivo studies, the GP polyphenols have a direct antioxidant activity by acting as a free radical scavenger or donating a hydrogen atom. It also possesses an indirect antioxidant and anti-inflammatory activity by reducing mitochondrial reactive oxygen species (ROS) generation, malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-α), interleukin-1-beta (IL-1β), interleukin-6 (IL-6), nuclear factor kappa-light-chain-enhancer of activated B cells (NF- κβ), and inhibitor of nuclear factor kappa-B kinase subunit beta (Iκκβ) levels or nitrate oxide-4 (NOX4) expression and by increasing the levels of antioxidants enzymes like superoxide dismutase (SOD), catalase (CAT) glutathione reductase (GRx) and glutathione peroxidase(GPx). Besides these activities, many beneficial effects in ischemic heart diseases were also observed, such as the maintenance of the ventricular function as close as possible to normal, and the prevention of infarcted area extension. In this context, this review intends to present the actual knowledge of grape pomace’s potential antioxidant and anti-inflammatory activity in ischemic heart disease, knowledge gathered from existing in vitro and in vivo studies focused on this.

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“…Since 2016 ( 1 ), a limited number of new molecules have been tested in animal model studies of IBD ( Table 2 ). These include betulinic acid ( 57 ), goat whey ( 58 ), polyphenols ( 59 ), eriocitrin ( 60 ), proglitazone ( 61 ), dietary products from grape seeds ( 62 , 67 ) and lupin extracts ( 63 ) and traditional Chinese herbal preparations ( 64 ). In clinical studies, a cannabinoid receptor 2 agonist was found to improve mucosal healing ( 65 ).…”

Section: Therapeutic Implicationsmentioning

confidence: 99%

How to place the duality of specific MMP-9 inhibition for treatment of inflammatory bowel diseases into clinical opportunities?

2022

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Crohn’s disease (CD) and ulcerative colitis (UC) are inflammatory bowel diseases (IBD) with the involvement of immune cells and molecules, including cytokines, chemokines and proteases. A previous extensive review about the molecular biology of matrix metalloproteases (MMPs) and tissue inhibitors of metalloproteases (TIMPs), related to intestinal barrier destruction and restoration functions in IBD, is here complemented with the literature from the last five years. We also compare IBD as a prototypic mucosal inflammation of an epithelial barrier against microorganisms with inflammatory retinopathy as a disease with a barrier dysfunction at the level of blood vessels. Multiple reasons are at the basis of halting clinical trials with monoclonal antibodies against MMP-9 for IBD treatment. These include (i) the absence of a causative role of MMP-9 in the pathology in animal models of IBD, (ii) the fact that endotoxins, crossing the intestinal barrier, induce massive local release of both neutrophil collagenase (MMP-8) and gelatinase B (MMP-9), (iii) insufficient recognition that MMPs modify the activities of cytokines, chemokines and their receptors, (iv) ignorance that MMPs exist as mixtures of proteoforms with different posttranslational modifications and with different specific activities and (v) the fact that MMPs and TIMPs act in an interactive network, possibly having also beneficial effects on IBD evolution. Nevertheless, inhibition of MMPs may be a useful therapeutic approach during specific IBD disease phases or in specific sub-phenotypes. This temporary “window of opportunity” for MMP-9 inhibition may be complemented by a locoregional one, provided that the pharmacological agents are targeted in time to affected tissues, as is achieved in ophthalmological inflammation. Thus, in order to discover spatial and temporal windows of opportunity for MMP inhibition as treatment of IBD, more preclinical work including well controlled animal studies will be further needed. In this respect, MMP-9/NGAL complex analysis in various body compartments is helpful for better stratification of IBD patients who may benefit from anti-MMP-9.

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Grape Pomace Extract Attenuates Inflammatory Response in Intestinal Epithelial and Endothelial Cells: Potential Health-Promoting Properties in Bowel Inflammation

Cited by 27 publications

References 85 publications

The Wine Industry By-Products: Applications for Food Industry and Health Benefits

The Wine Industry By-Products: Applications for Food Industry and Health Benefits

Antioxidant and Anti-Inflammatory Actions of Polyphenols from Red and White Grape Pomace in Ischemic Heart Diseases

How to place the duality of specific MMP-9 inhibition for treatment of inflammatory bowel diseases into clinical opportunities?

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