Grape cytochrome P450 CYP90D1 regulates brassinosteroid biosynthesis and increases vegetative growth

Enoki, Shinichi; Tanaka, Keisuke; Moriyama, Ayane; Hanya, Norimichi; Mikami, Norika; Suzuki, Shunji

doi:10.1016/j.plaphy.2023.02.052

Cited by 8 publications

(3 citation statements)

References 35 publications

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“…Plants with BRs metabolic mutants and overexpression exhibited rapid growth, including larger leaves, more height, and increased biomass [7,18]. These phenotypic alterations are consistent with previous reports on transgenic Arabidopsis overexpressing AtCYP90D1, which displayed increased inflorescence height and seed and branch counts [40]. Similarly, overexpression of Populus trichocarpa CYP85A3, a key enzyme gene for BRs synthesis, in tomatoes increased the height of transgenic plants by 10 ~ 20 cm, which was 2.17 times that of wild type.…”

Section: Discussionsupporting

confidence: 90%

“…Therefore, the high expression of PtoCYP90D1 in old leaves and low expression in young leaves indicated the important role of PtoCYP90D1 in leaf maturation and development. In a previous study, overexpression of AtCYP90D1 in transgenic Arabidopsis plants increased plant height and internode length [40], while internode cell shortening was reported forOsCYP90D1 RNA interference mutant [28]. The present study showed that overexpression of PtoCYP90D1 resulted in an increase in both plant height and stem diameter in poplar, whereas knock-out of PtoCYP90D1 did not exhibit a significant difference in height or stem diameter as compared to wild type (Figure 4).…”

Section: Discussionsupporting

confidence: 63%

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Molecular cloning and characterization of a brassinosteriod biosynthesis-related gene PtoCYP90D1 from Populus tomentosa

Liu,

Tian,

Tang

et al. 2024

Preprint

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Brassinosteroids (BRs), one of the major classes of phytohormones are essential for various processes of plant growth, development, and adaptations to biotic and abiotic stresses. In Arabidopsis, AtCYP90D1 acts as a bifunctional cytochrome P450 monooxygenase, catalyzing C-23 hydroxylation in the brassinolide biosynthetic pathway. The present study reports the functional characterizations of PtoCYP90D1, one of the AtCYP90D1 homologous genes from Populus tomentosa. The qRT-PCR analysis showed that PtoCYP90D1 was highly expressed in roots and old leaves. Overexpression of PtoCYP90D1 (PtoCYP90D1-OE) in poplar promoted growth and biomass yield, as well as increased xylem area and cell layers. Transgenic plants exhibited a significant increase in plant height and stem diameter as compared to the wild type. In contrast, the CRISPR/Cas9-generated mutation of PtoCYP90D1 (PtoCYP90D1-KO) resulted in significantly decreased biomass production in transgenic plants. Further studies revealed that cell wall components increased significantly in PtoCYP90D1-OE lines but not in PtoCYP90D1-KO lines, as compared to wild-type plants. Overall, the findings indicate a positive role of PtoCYP90D1 in improving growth rate and elevating biomass production in poplar, which will have positive implications for its versatile industrial or agricultural applications.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 63%

Molecular cloning and characterization of a brassinosteriod biosynthesis-related gene PtoCYP90D1 from Populus tomentosa

Liu,

Tian,

Tang

et al. 2024

Preprint

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“…In this test, the application of FTHSB has signi cantly increased the use e ciency of N in different forms probably because such brick has a developed pore structure and therefore achieves a strong adsorption capacity. This special structure can reduce the solution and migration of water-soluble nutrient, avoid the leaching loss of nutrient, realize the sustained release of nutrient in root zones and improve the porosity and nutrient distribution uniformity in soil pro les(Enoki et al 2023;Kengdo et al 2023;Li et al 2021). In the treatment without FTHSB but with 3 forms of N, nitrate N has the highest NUE and ammonium N has the lowest NUE.…”

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confidence: 99%

Tracing N utilization and distribution during development of grape seedlings by applying different forms of 15N labeled fertilizer with fruit tree hole storage brick

Yao,

jia,

Zhang

et al. 2024

Preprint

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Background and aims Application of soil conditioner can promote the NUE in plants by increasing N retention in soil as an effective method to reduce chemical fertilizer consumption and environmental pollution. Methods We have prepared fruit tree hole storage brick (FTHSB) suitable for perennial fruit trees using natural nanomaterials, vermiculite and montmorillonite, and organic fertilizers, then recommended a new fertilization strategy combining N fertilizer and FTHSB based on subsurface drip irrigation. Results Application of FTHSB significantly increased the amount of dry matter in each organ of grape (Vitis vinifera L.) seedlings, significantly promoted fine root growth of soil layer in the range of 15–30 cm, reduced the leaching loss of 15N to deep soil and significantly improved the use efficiency of 15N. In the treatment without FTHSB, NO3−-N is better than NH4+-N and CO(NH2)2-N in terms of plant growth index of soil layer in the range of 15–30 cm and 15N use efficiency in plants. After the application of FTHSB, CO(NH2)2-N is better than NO3−-N and NH4+-N according to the above indicators. Conclusions In general, compared with ammonium and nitrate N FTHSB had the strongest adsorption capacity for amide N, so the N content of the soil in the vicinity of the FTHSB with amide N was also the highest, which improved the dry matter of all organs, the growth of the fine roots and the efficiency of N utilization in the grape seedlings.

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Integrated omics approach reveals the molecular pathways activated in tomato by Kocuria rhizophila, a soil plant growth-promoting bacterium

Mauceri,

Puccio,

Faddetta

et al. 2024

Plant Physiology and Biochemistry

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Grape cytochrome P450 CYP90D1 regulates brassinosteroid biosynthesis and increases vegetative growth

Cited by 8 publications

References 35 publications

Molecular cloning and characterization of a brassinosteriod biosynthesis-related gene PtoCYP90D1 from Populus tomentosa

Molecular cloning and characterization of a brassinosteriod biosynthesis-related gene PtoCYP90D1 from Populus tomentosa

Tracing N utilization and distribution during development of grape seedlings by applying different forms of 15N labeled fertilizer with fruit tree hole storage brick

Integrated omics approach reveals the molecular pathways activated in tomato by Kocuria rhizophila, a soil plant growth-promoting bacterium

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