Grape Cane Extracts as Multifunctional Rejuvenating Cosmetic Ingredient: Evaluation of Sirtuin Activity, Tyrosinase Inhibition and Bioavailability Potential

Abstract

Grape canes are waste biomass of viticulture containing bioactive polyphenols valuable in cosmetics. Whereas several studies reported the cosmetic activities of E-resveratrol, only few described the potential of E-ε-viniferin, the second major constituent of grape cane extracts (GCE), and none of them investigated GCE as a natural blend of polyphenols for cosmetic applications. In this study, we considered the potential of GCE from polyphenol-rich grape varieties as multifunctional cosmetic ingredients. HPLC analysis was performed to quantify major polyphenols in GCE i.e., catechin, epicatechin, E-resveratrol, E-piceatannol, ampelopsin A, E-ε-viniferin, hopeaphenol, isohopeaphenol, E-miyabenol C and E-vitisin B from selected cultivars. Skin whitening potential through tyrosinase inhibition assay and the activation capacity of cell longevity protein (SIRT1) of GCE were compared to pure E-resveratrol and E-ε-viniferin. Drug-likeness of GCE polyphenols were calculated, allowing the prediction of skin permeability and bioavailability. Finally, the present data enabled the consideration of GCE from polyphenol-rich varieties as multifunctional cosmetic ingredients in accordance with green chemistry practices.

Characterization and Optimization of the Tyrosinase Inhibitory Activity of Vitis amurensis Root Using LC-Q-TOF-MS Coupled with a Bioassay and Response Surface Methodology

“…Both compounds showed higher tyrosinase inhibitory effects than the positive control, kojic acid which is the known skin whitening constituent ( Table 1 and Figure S4 ). In previous studies, ε-viniferin ( 1 ) is reported to have tyrosinase inhibitory activity [ 23 ]; however, vitisin B ( 2 ) was first identified in our study.…”

“…First, two constituents expected to have tyrosinase inhibitory activity were isolated from the EtOAc fraction and their bioactivity evaluated. The structures of the isolated compounds 1 and 2 were identified as ε-viniferin ( 1 ) [ 20 , 21 ] and vitisin B (γ-viniferin, 2 ) [ 21 , 22 , 23 ], respectively, using 1 H-NMR, 13 C-NMR, and ESI-MS ( Figure 2 , Figures S2 and S3, and Table S2 ). In tyrosinase inhibitory assay, the IC 50 values of compounds 1 , 2 , and kojic acid were 3.51, 10.74, and 27.09 µM, respectively.…”

“…As a result, it was confirmed that compounds can be inserted into the active site of the target protein and bind to catalytic amino acid residues that can inhibit tyrosinase activity. Additionally, ε-viniferin ( 1 ) is reported as a competitive inhibitor that binds to the same site that L-DOPA binds to tyrosinase [ 23 ]. It was observed that vitisin B ( 2 ) binds to the same site as ε-viniferin ( 1 ), which confirmed that vitisin B ( 2 ) is a new competitive inhibitor.…”

Nano-Elicitation as an Effective and Emerging Strategy for In Vitro Production of Industrially Important Flavonoids

“…Flavonoids possess significant antiallergic, antihepatotoxic, anti-inflammatory, antioxidant, antitumor, antiviral, antibacterial, antiosteoporosis as well as cardio-protective activity. Antioxidant, antihepatotoxic, anti-inflammatory, antiallergic, antitumor, antiosteoporosis, antiviral, antibacterial and cardio-protective potential have been reported in many flavonoids from various plant species [5][6][7][8][9]. In vivo studies also support the possible use of flavonoid rich plants in treating diseases.…”

Grape Canes from Typical Cultivars of Campania (Southern Italy) as a Source of High-Value Bioactive Compounds: Phenolic Profile, Antioxidant and Antimicrobial Activities

“…Currently, few possible applications for this residue are reported; some examples include as a precursor of activated carbon or raw material for the production of biosurfactants [ 9 , 10 ]. Conversely, the exploitation of grape canes as a novel and cheap source of bioactive compounds, such as polyphenols, is poorly investigated, and the potential of grape cane extracts as multifunctional active ingredients in cosmetic formulations for skin care was very recently described [ 11 ]. These molecules, thanks to their well-known strong antioxidant power, are of significant interest for use in the food, cosmetic and pharmaceutical industries [ 12 ].…”