Granulosicoccus marinus sp. nov., isolated from Antarctic seawater, and emended description of the genus Granulosicoccus

Abstract

A Gram-staining-negative, motile by flagella, non-pigmented, poly-b-hydroxybutyrate-producing, strictly aerobic and sphere-shaped bacterium, IMCC3490 T , was isolated from a coastal seawater sample from the Antarctic Peninsula. Optimal growth of strain IMCC3490T was observed at 20 6C, pH 8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis using 16S rRNA gene sequences indicated that strain IMCC3490 T belonged to the genus Granulosicoccus in the family Granulosicoccaceae. The strain was closely related to Granulosicoccus antarcticus IMCC3135 T (98.8 % 16S rRNA gene sequence similarity) and Granulosicoccus coccoides Z 271 T (98.5 %). The DNA-DNA relatedness values between IMCC3490 T and type strains of the two species of the genus were far lower than 70 %, which indicated strain IMCC3490 T is a novel genomic species of the genus Granulosicoccus. The major fatty acids of strain IMCC3490 T were summed feature 8 (C 18 : 1 v7c and/or C 18 : 1 v6c) and summed feature 3 (C 16 : 1 v7c and/or C 16 : 1 v6c). The isoprenoid quinone detected was ubiquinone-8 (Q-8) and predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 61.0 mol%. On the basis of phylogenetic analyses, DNA-DNA relatedness values and phenotypic data, it is suggested that strain IMCC3490 T represents a novel species of the genus Granulosicoccus, for which the name Granulosicoccus marinus sp. nov. is proposed.

Functional Insights into the Kelp Microbiome from Metagenome-Assembled Genomes

“…The genus Granulosicoccus (order Chromatiales , class Gammaproteobacteria ) currently contains 4 species isolated from Antarctic seawater ( 67 , 68 ), the seagrass Zostera marina ( 69 ), and the kelp Undaria pinnatifida ( 70 ). Granulosicoccus is the most abundant bacterium associated with N. luetkeana ( 9 ), reaching densities of up to 10 6 cells per cm 2 on kelp blades ( 5 ), and it associates with diverse algal hosts ( 18 ).…”

Successional Dynamics and Seascape-Level Patterns of Microbial Communities on the Canopy-Forming Kelps Nereocystis luetkeana and Macrocystis pyrifera

“…( Gammaproteobacteria ). Bacteria from the genus Granulosicoccus have been isolated from seagrass (Kurilenko et al, 2010) and brown algae (Park et al, 2014), and they have been characterized as aerobic, chemoheterotrophic bacteria that are capable of nitrate reduction to nitrite (Baek et al, 2014; Park et al, 2014). Three of the four most abundant ASVs on N. luetkeana were classified as Granulosicoccus sp., indicating that there are multiple, closely related sequence variants that dominate the microbiome of N. luetkeana.…”

Temporal covariation of epibacterial community and surface metabolome in the Mediterranean seaweed holobiont Taonia atomaria

“…), the link between the two clusters rests on the genus Granulosicoccus . The latter has been identified as a psychrotrophic taxon, widely reported from Antarctic coastal seawaters (Baek et al ., ) and sediments (Wang et al ., ) but also from the surface of an unidentified brown alga (Park et al ., ) or the Mediterranean Chlorophyceae Caulerpa spp. (Rizzo et al ., ).…”