Granulosa cell function and oocyte competence: Super-follicles, super-moms and super-stimulation in cattle

Dias, F. C. F.; Khan, M. Islam; Adams, Gregg P.; Sirard, Marc‐André; Singh, Jaswant

doi:10.1016/j.anireprosci.2014.07.016

Cited by 30 publications

(27 citation statements)

References 92 publications

(108 reference statements)

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“…At the same time, the reduction in prostaglandin E 2 might impair ovulation as these prostaglandins are absolutely essentials for the ovulatory process. In a different study by (Dias et al 2014) a prolonged coasting period (more than 96 h) has resulted in a decreased ovulation rate and would support our suggestion. In other words, the expression from GC of some inflammation-related molecules seems to reflect the destiny of the follicle, atresia of ovulation.…”

Section: Lh and Ovulationsupporting

confidence: 80%

Influence of luteinizing hormone support on granulosa cells transcriptome in cattle

Nivet

Vigneault

Blondin

et al. 2017

Animal Science Journal

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In cows, the use of follicle-stimulating hormone (FSH) to stimulate follicular growth followed by a short period of FSH withdrawal has been shown to be beneficial for oocyte developmental competence. Although this treatment represents a useful optimization to generate highly competent oocytes, the underlying physiological process is not completely understood. The goal of this study was to investigate the role of luteinizing hormone (LH) action during FSH withdrawal before ovulation. To accomplish this, LH release was pharmacologically inhibited during the coasting period with gonadotropin-releasing hormone (GnRH) antagonists. Granulosa cells samples were obtained from cows stimulated with FSH during 3 days followed by a coasting period of 68 h and treated with a GnRH antagonist (cetrorelix group) or not (control). A significant reduction in the number of follicles at >10 mm diameter was observed with the cetrorelix group and gene expression of granulosa cells reveals that 747 transcripts are potentially regulated by LH. Further analysis indicates how the absence of LH may trigger early atresia, the upregulation of atretic agent as tumor protein P53 and transforming growth factor β1 and the inhibition of growth support. This work allows identification of genes that are associated with maintained follicular growth and conversely the ones leading to atresia in dominant pre-ovulatory follicles.

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Section: Lh and Ovulationsupporting

confidence: 80%

Influence of luteinizing hormone support on granulosa cells transcriptome in cattle

Nivet

Vigneault

Blondin

et al. 2017

Animal Science Journal

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“…The vast variability in the ovarian response and subsequent embryos production in MOET programs are related to many factors includes gonadotrophin sources and preparation [5][6][7][8][9], super-stimulation protocol [10], follicular population and its dynamics growth at the time of treatment initiation [23,24], farm management [25], nutritional status [26,27], genetic merit for fertility traits [28][29][30] and environmental conditions [31]. It has been demonstrated that FSH:LH ratio affects the ability of the ovary to respond to exogenous gonadotrophin treatment [4].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the superovulatory response fortuity is still represents obstacles facing the efficiency and profitability of MOET program during practical application [4]. These variability have been attributed to gonadotrophin preparation with differences LH:FSH activities [5][6][7][8][9] and its protocols for treatments [10,11]. In addition, antral follicle count and AMH level are contributed to these variability's [12,13].…”

Section: Introductionmentioning

confidence: 99%

Impact of Type and Dose of Recombinant Human Follicle Stimulating Hormone on Embryos Production in Dairy Cows and Their Relationship with AMH Concentration

Mohammed¹,

Darwish²,

Dohreig³

et al. 2019

AVS

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The study was investigated to compare the effectiveness of Folltropin and two products (Fostimon and Menogon) of a recombinant human follicle stimulating hormone (rhFSH) on ovarian super-stimulation and embryo production in dairy Holstein cows. Two different doses for each super-ovulatory product were investigated. The relationship between the levels of Anti-Mullerian Hormone (AMH) in the blood serum before super-stimulation initiation and ovarian response and embryos production were also evaluated. At random stage of the estrus cycle, the donor cows were received intra-vaginal controlled internal drug release device (CIDR) and injected with 100µg of gonadotropin-releasing hormone (GnRH). On fourth day of CIDR insertion, cows were divided according to the product types of superovulatory hormones into three equal main groups. According to hormonal dose, each main group was re-divided into two equally subgroups ; low and high dose. Superovulatory hormones was injected IM as decreasing doses; 40%, 30%, 20% and 10% of the total dose twice daily for 4 days. On day 7, CIDRs were removed and each cow received 2 doses of PGF 2 α with 12hr interval. Based upon heat detection, donor's cows were inseminated with frozen semen three times 8-10hrs interval and concurrent with 100µg GnRH injection at first insemination. Non-surgical embryos recovery was performed on day's 7-8 post insemination and ovarian super-stimulation was checked using sonography to estimate ovarian response. Recovered embryos were morphologically evaluated as grades. Twelve embryos collected from Menogon donors group were transferred to six recipient's cows. The mean numbers of CL, recovered embryo/ova, fertilized ova and transferable embryos are significantly (P<0.01) higher in Folltropin and Menogon treated cows than Fostimon group. Donor cows treated with high doses of Folltropin and Menogon showed higher means for transferable and freezable embryos. Three recipients (50%) who received embryos appeared pregnant and one of them born calf. Significant positive correlations were reported between AMH on one side and CL, total ovarian response, transferable and freezable embryos on the other side, and cows having AMH≥230ng/L exhibited significant higher means. In conclusion: Menogon can be used as rhFSH for embryos production with transferable quality. AMH can be used as indicator to helpful select cow's candidates as donors. More studies are needed using Fostimon accompanied with different levels of LH.

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“…In mouse GCs, SMAD7 promotes GC apoptosis, while reduction of SMAD7 blocks TGF‐β‐induced apoptosis (Quezada et al, ). In addition, SMAD7 is closely related to many mediators of GC apoptosis, including FSH (Dias, Khan, Adams, Sirard, & Singh, ), AMHR2 (Li et al, ), norepinephrine (Zhang, Gao, & Cui, ), and the TGF‐β superfamily (Gao et al, ). Norepinephrine is thought to be a potent moderator of ovarian functions; a recent study showed it controls GC apoptosis via miR‐21 and the SMAD7 axis in mouse GCs (Zhang et al, ).…”

Section: Discussionmentioning

confidence: 99%

miR‐181b‐induced SMAD7 downregulation controls granulosa cell apoptosis through TGF‐β signaling by interacting with the TGFBR1 promoter

Wang

Pan

et al. 2018

Journal Cellular Physiology

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SMAD7 disrupts the TGF-β signaling pathway by influencing TGFBR1 stability and by blocking the binding of TGFBR1 to SMAD2/3. In this study, we showed that SMAD7 attenuated the TGF-β signaling pathway in ovarian granulosa cells (GCs) by regulating TGFBR1 transcriptional activity. To function as a transcription factor, SMAD7 downregulated the mRNA levels of TGFBR1 via direct binding to the SMAD-binding elements (SBEs) within the promoter region of pig TGFBR1. We also showed that SMAD7 enhanced porcine GC apoptosis by interrupting TGFBR1 and the TGF-β signaling pathway. Interestingly, miR-181b, a microRNA that is downregulated during porcine follicular atresia, was identified to be directly targeting SMAD7 at its 3'-UTR. By inhibiting SMAD7, miR-181b could inhibit GC apoptosis by activating the TGF-β signaling pathway. Our findings provide new insights into the mechanisms underlying the regulation of the TGF-β signaling pathway by SMAD7 and miR-181b.

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Granulosa cell function and oocyte competence: Super-follicles, super-moms and super-stimulation in cattle

Cited by 30 publications

References 92 publications

Influence of luteinizing hormone support on granulosa cells transcriptome in cattle

Influence of luteinizing hormone support on granulosa cells transcriptome in cattle

Impact of Type and Dose of Recombinant Human Follicle Stimulating Hormone on Embryos Production in Dairy Cows and Their Relationship with AMH Concentration

miR‐181b‐induced SMAD7 downregulation controls granulosa cell apoptosis through TGF‐β signaling by interacting with the TGFBR1 promoter

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