Granulocyte functions and changes in ability with age in newborns; Report no. 1: Flow cytometric analysis of granulocyte functions in whole blood

Moriguchi, Naohiko; Yamamoto, Seiichirou; Isokawa, Sadayuki; Andou, Atushi; Miyata, Hiroshi

doi:10.1111/j.1442-200x.2006.02151.x

Cited by 9 publications

(10 citation statements)

References 21 publications

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“…Decreased intake of bacteria might be due to reduced levels of IgG antibodies and complement proteins resulting in impaired opsonization and/or reduced expression of relevant receptors [9,15-21]. The bacterial killing might be reduced even when their intake is normal, due to decreased intracellular production of oxidative radicals [17,22]. …”

Section: Introductionmentioning

confidence: 99%

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Phagocytic ability of neutrophils and monocytes in neonates

et al. 2011

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BackgroundInfections by a variety of pathogens are a significant cause of morbidity and mortality during perinatal period. The susceptibility of neonates to bacterial infections has been attributed to immaturity of innate immunity. It is considered that one of the impaired mechanisms is the phagocytic function of neutrophils and monocytes. The purpose of the present study was to investigate the phagocytic ability of neonates at birth.MethodsThe phagocytic ability of neutrophils and monocytes of 42 neonates was determined using the Phagotest flow cytometry method, that assesses the intake of E. Coli by phagocytes, in cord blood and in peripheral blood 3 days after birth. Fifteen healthy adults were included in the study as controls.ResultsThe phagocytic ability of neutrophils in the cord blood of neonates was significantly reduced compared to adults. The 3rd postnatal day the reduction of phagocytic ability of neutrophils was no longer significant compared to adults. The phagocytic ability of monocytes did not show any difference from that of adults either at birth or the 3rd postnatal day.ConclusionsOur findings indicate that the intake of E. Coli by phagocytes is impaired at birth in both preterm and full term neonates compared to adults. This defect is transient, with the phagocytic ability in neonates reaching that of the adults 3 days after birth.

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Section: Introductionmentioning

confidence: 99%

“…Recent reports using flow cytometry techniques confirm the defects on aspects of intracellular killing reported by previous techniques, but they do not add information on the phagocytic activity of neonatal neutrophils [22,23]. …”

Section: Introductionmentioning

confidence: 99%

Phagocytic ability of neutrophils and monocytes in neonates

et al. 2011

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“…After extravasation, the neutrophil follows chemokine gradients through the tissue to the point of damage/inflammation. Developmental alterations of adhesion molecule expression are indicated by footnotes as follows (fetal/premature levels were compared to those of adults): ( a ) L-selectin - reduced [26, 33, 37, 45, 46] and unchanged [47], E-selectin - reduced [29, 31, 38], P-selectin - reduced [29, 34, 38, 39]; ( b ) PSGL-1 - reduced [29, 31, 36]; ( c ) Mac-1 - reduced [26, 31, 36], unchanged [30, 33], and increased [37]; ( d ) LFA-1 - reduced [26, 45] and unchanged [30, 31, 36, 37]; ( e ) ICAM-1 - reduced [29, 31, 38]; ( f ) CXCR2 - unchanged [31] …”

Section: Leukocyte Recruitmentmentioning

confidence: 99%

“…Notably, cellular immunity of preterm infants matures slower and later than in term infants [11]. Expression of L-, P-, and E-selectin was reported to be reduced in mature neonates in vitro and in vivo compared to infants and adults [31, 33, 34]. On the other hand, posttranslational glycosylation of selectin ligands is augmented during the neonatal period [35].…”

Section: Maturation Of Fetal Leukocyte Recruitmentmentioning

confidence: 99%

“…On the other hand, posttranslational glycosylation of selectin ligands is augmented during the neonatal period [35]. In most studies, fetal expression of integrins such as Mac-1 and LFA-1 were described to be reduced or equal compared to adults [26, 30, 31, 33, 36, 37]. Similar observations have been described for other adhesion molecules such as ICAM-1/-2, VCAM-1, VLA4, and PSGL-1 [29, 31, 38, 39].…”

Section: Maturation Of Fetal Leukocyte Recruitmentmentioning

confidence: 99%

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Leukocyte recruitment in preterm and term infants

2016

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Impaired cellular innate immune defense accounts for susceptibility to sepsis and its high morbidity and mortality in preterm infants. Leukocyte recruitment is an integral part of the cellular immune response and follows a well-defined cascade of events from rolling of leukocytes along the endothelium to firm adhesion and finally transmigration which is concerted by a variety of adhesion molecules. Recent analytical advances such as fetal intravital microscopy have granted new insights into ontogenetic regulation and maturation of fetal immune cell recruitment. Understanding the fetal innate immune system is essential for targeted prevention and therapy of premature infants with severe infections or disorders of the immune system. This review gives an overview of the basic principles of leukocyte recruitment, particularly neutrophil trafficking, and its development during early life and highlights technical limitations to our current knowledge.

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The soluble cytoplasmic tail of CD45 (ct‐CD45) in human plasma contributes to keep T cells in a quiescent state

et al. 2016

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The cytoplasmic tail of CD45 (ct‐CD45) is proteolytically cleaved and released upon activation of human phagocytes. It acts on T cells as an inhibitory, cytokine‐like factor in vitro. Here, we show that ct‐CD45 is abundant in human peripheral blood plasma from healthy adults compared with plasma derived from umbilical cord blood and plasma from patients with rheumatoid arthritis or systemic lupus erythematosus. Plasma depleted of ct‐CD45 enhanced T‐cell proliferation, while addition of exogenous ct‐CD45 protein inhibited proliferation and reduced cytokine production of human T lymphocytes in response to TCR signaling. Inhibition of T‐cell proliferation by ct‐CD45 was overcome by costimulation via CD28. T‐cell activation in the presence of ct‐CD45 was associated with an upregulation of the quiescence factors Schlafen family member 12 (SLFN12) and Krueppel‐like factor 2 (KLF2) as well as of the cyclin‐dependent kinase (CDK) inhibitor p27kip1. In contrast, positive regulators of the cell cycle such as cyclin D2 and D3 as well as CDK2 and CDK4 were found to be downregulated in response to ct‐CD45. In summary, we demonstrate that ct‐CD45 is present in human plasma and sets the threshold of T‐cell activation.

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Granulocyte functions and changes in ability with age in newborns; Report no. 1: Flow cytometric analysis of granulocyte functions in whole blood

Abstract: Decreased functions of part of the phagocyte system during early infancy were clearly observed.

Cited by 9 publications

References 21 publications

Phagocytic ability of neutrophils and monocytes in neonates

Phagocytic ability of neutrophils and monocytes in neonates

Leukocyte recruitment in preterm and term infants

The soluble cytoplasmic tail of CD45 (ct‐CD45) in human plasma contributes to keep T cells in a quiescent state

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