Granulocyte-colony stimulating factor promotes proliferation, migration and invasion in glioma cells

Wang, Juntao; Yao, Lishuang; Zhao, Shidou; Zhang, Xiaodan; Yin, Junyi; Zhang, Yanmin; Chen, Xueran; Gao, Ming; Ling, Eng‐Ang; Hao, Aijun; Li, Gang

doi:10.4161/cbt.19237

Cited by 55 publications

(52 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although G-CSF behaves as a potent migratory stimulus for haematopoietic cells [48], just a moderate effect was observed in Swan 71 cells. In this regard, it should be considered that a wide range of G-CSF migratory potencies has been reported in non-haematopoietic tissues, being slight responses (up to 1.4-fold increase) induced in glioma cells [7] and head and neck squamous cell carcinomas [8], and more pronounced effects (up to 6-fold increase) observed in rat oval cells [49] and gastric and colon cancer cells [50]. In spite of the differences observed in the G-CSF-migratory potency, this cytokine might be considered as a promoting factor for cell migration, while other G-CSF-mediated biological effects, such as the proliferative and survival responses, seem to be more restricted to some cell types.…”

Section: Discussionmentioning

confidence: 99%

“…Even though the function of G-CSF on the regulation and mobilization of neutrophils has been extensively studied [2-4], G-CSF-triggered biological actions are not exclusively restricted to haematopoietic tissues. Thus, it has been reported that G-CSF induced the migration of endothelial [5,6], glioma [7] and tumor cells [8-10]. In addition, G-CSF signaling enhances the survival of cardiomyocytes [11] and stimulates neurogenesis [12].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation

Furmento

Marino

Blank

et al. 2016

Experimental Cell Research

View full text Add to dashboard Cite

Multiple cytokines and growth factors expressed at the fetal-maternal interface are involved in the regulation of trophoblast functions and placental growth, but the role of G-CSF has not been completely established. Based on our previous study showing that G-CSF increases the activity of matrix metalloproteinase-2 and the release of vascular endothelial growth factor in Swan 71 human trophoblast cells, in this work we explore the possible contribution of G-CSF to cell migration and the G-CSF-triggered signaling pathway. We found that G-CSF induced morphological changes on actin cytoskeleton consistent with a migratory cell phenotype. G-CSF also up-regulated the expression levels of β1 integrin and promoted Swan 71 cell migration. By using selective pharmacological inhibitors and dominant negative mutants we showed that PI3K, Erk 1/2 and p38 pathways are required for promoting Swan 71 cell motility. It was also demonstrated that PI3K behaved as an upstream regulator of Erk 1/2 and p38 MAPK. In addition, the increase of β1 integrin expression was dependent on PI3K activation. In conclusion, our results indicate that G-CSF stimulates β1 integrin expression and Swan 71 cell migration by activating PI3K and MAPK signaling pathways, suggesting that G-CSF should be considered as an additional regulatory factor that contributes to a successful embryo implantation and to the placenta development.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation

Furmento

Marino

Blank

et al. 2016

Experimental Cell Research

View full text Add to dashboard Cite

show abstract

“…GM-CSF is upregulated in both human and mouse glioma microenvironments where it upregulates immunosuppressive IL-4 receptors on MDSC in that microenvironment. 83 Wang et al 84 demonstrated that antibody neutralization of G-CSF inhibited growth of glioma cells in vitro. Activation of STAT3 as well as expression of several of its downstream effectors was stimulated by G-CSF contributing to the observed sustained proliferative signaling in these GB cell lines, 84 predicting benefit from dapsone, fenofibrate, and ribavirin.…”

Section: G(m)-csf Is a Direct Growth Factor For Gbmentioning

confidence: 99%

Glioblastoma-synthesized G-CSF and GM-CSF contribute to growth and immunosuppression: Potential therapeutic benefit from dapsone, fenofibrate, and ribavirin

Kast¹,

Hill

Wion

et al. 2017

Tumour Biol.

View full text Add to dashboard Cite

Increased ratio of circulating neutrophils to lymphocytes is a common finding in glioblastoma and other cancers. Data reviewed establish that any damage to brain tissue tends to cause an increase in G-CSF and/or GM-CSF (G(M)-CSF) synthesized by the brain. Glioblastoma cells themselves also synthesize G(M)-CSF. G(M)-CSF synthesized by brain due to damage by a growing tumor and by the tumor itself stimulates bone marrow to shift hematopoiesis toward granulocytic lineages away from lymphocytic lineages. This shift is immunosuppressive and generates the relative lymphopenia characteristic of glioblastoma. Any trauma to brain-be it blunt, sharp, ischemic, infectious, cytotoxic, tumor encroachment, or radiation-increases brain synthesis of G(M)-CSF. G(M)-CSF are growth and motility enhancing factors for glioblastomas. High levels of G(M)-CSF contribute to the characteristic neutrophilia and lymphopenia of glioblastoma. Hematopoietic bone marrow becomes entrained with, directed by, and contributes to glioblastoma pathology. The antibiotic dapsone, the lipid-lowering agent fenofibrate, and the antiviral drug ribavirin are Food and Drug Administration-and European Medicines Agency-approved medicines that have potential to lower synthesis or effects of G(M)-CSF and thus deprive a glioblastoma of some of the growth promoting contributions of bone marrow and G(M)-CSF.

show abstract

“…We do know, however that the mobilization of dendritic precursor cells, mature dendritic cells, and native killer cells are essential [23]. Although this possibility has been raised, especially concerning hematologic neoplasms, we know of no reports that suggest that this is true.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Granulocyte Macrophage-Colony Stimulating Factor upon the Induction of Peripheral Blood Dendritic and Natural Killer Cells When Given Simultaneously with a Slow Continuous Doxorubicin Infusion

Weiss¹,

Stoloff²,

Simoes³

2017

JCT

View full text Add to dashboard Cite

It has been demonstrated that it is safe to give Gm-Csf, together with Doxorubicin, by continuous intravenous infusion, thereby substantially increasing the amount of Doxorubicin administered to the average patient, and assuring that each patient receives an individually-determined safe and maximal amount of drug. It is known that Gm-Csf is a potent inducer of components that are major factors in an immunologic attack upon neoplasms. For that reason, we thought it would be worth evaluating in 4 patients' surface markers of dendritic precursor cells, dendritic cells [DC], and natural killer [NK] cells during the infusion. While there was substantial variation in individual responses, all 4 patients receiving Gm-Csf developed persistent marked increases in cells with each of these markers. The significance of these findings will be discussed.

show abstract

Granulocyte-colony stimulating factor promotes proliferation, migration and invasion in glioma cells

Cited by 55 publications

References 38 publications

Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation

Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation

Glioblastoma-synthesized G-CSF and GM-CSF contribute to growth and immunosuppression: Potential therapeutic benefit from dapsone, fenofibrate, and ribavirin

The Effect of Granulocyte Macrophage-Colony Stimulating Factor upon the Induction of Peripheral Blood Dendritic and Natural Killer Cells When Given Simultaneously with a Slow Continuous Doxorubicin Infusion

Contact Info

Product

Resources

About