Granulocyte-colony stimulating factor induced intranuclear endonuclease in murine leukemia cell line

Handa, Ajay; Kashimura, Takuya; Yamamoto, Akiko; Murohashi, Ikuo; Bessho, Masami; Hirashima, Kunitake

doi:10.1016/s0145-2126(00)00074-6

Cited by 5 publications

(4 citation statements)

References 29 publications

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“…G-CSF is a hematopoietic growth factor that stimulates the proliferation and differentiation of hematopoietic progenitor cells, activates the function of mature neutrophils, and prevents apoptosis [15][16][17][18]. G-CSF is currently used clinically to reduce the risk of life-threatening infection in patients with neutropenia, particularly after intense cytotoxic chemotherapy, radiotherapy and bone marrow transplantation [16,17].…”

Section: Introductionmentioning

confidence: 99%

Structural features in carrageenan that interact with a heparin-binding hematopoietic growth factor and modulate its biological activity

Liang

Zhou

Wang

et al. 2006

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Structural features in carrageenan that interact with a heparin-binding hematopoietic growth factor and modulate its biological activity

Liang

Zhou

Wang

et al. 2006

Journal of Chromatography B

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“…Besides, G-CSF augments cytarabine and etoposide-triggered apoptosis in leukemia cells [ 33 , 34 ]. Moreover, G-CSF triggers apoptosis in radiation-induced murine leukemia cell line C2M-A5 [ 35 ]. Except leukemia cells, G-CSF also induces apoptosis of B cells in bone marrow derived from healthy donors [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Combination regimen of granulocyte colony-stimulating factor and recombinant human thrombopoietin improves the curative effect on elderly patients with leukemia through inducing pyroptosis and ferroptosis of leukemia cells

2022

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Leukemia ranks as the one of most common causes of death from tumor. 51.4% of patients with leukemia are over 65 years old. However, the median overall survival (OS) of elderly leukemia patients is less than one year. It is urgent to explore more effective treatments for elderly patients with leukemia. Our recent prospective phase II single-arm study has revealed that combination regimen of granulocyte colony-stimulating factor (G-CSF) and recombinant human thrombopoietin (rhTPO) could improve the curative effect on elderly patients with leukemia, yet the precise mechanism remains unknown. This study demonstrated that combination of G-CSF and rhTPO showed greater effect on suppressing leukemia growth than G-CSF or rhTPO alone in vitro and in vivo. Mechanistically, G-CSF induced pyroptosis through ELANE in leukemia cells. Besides, rhTPO triggered ferroptosis by EP300 in leukemia cells. Moreover, rhTPO suppressed glutathione peroxidase 4 (GPX4) expression to induce ferroptosis through blocking the interaction between EP300 and GPX4 gene promoter via associating with EP300. In summary, this study illuminated that combination regimen of G-CSF and rhTPO improved the curative effect on elderly patients with leukemia through inducing pyroptosis and ferroptosis of leukemia cells. Therefore, our results provided a theoretical basis for combination regimen of G-CSF and rhTPO treating leukemia and potential therapeutic targets for leukemia.

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“…Granulocyte colony-stimulating factor (G-CSF) is a hematopoietic growth factor, which stimulates the proliferation and differentiation of hematopoietic progenitor cells, activates the function of mature neutrophils, and prevents apoptosis [23][24][25][26][27][28]. G-CSF is currently used clinically to reduce the risk of life-threatening infection in patients with neutropenia, particularly after intense cytotoxic chemotherapy, radiotherapy, and bone marrow transplantation [25,26].…”

Section: Introductionmentioning

confidence: 99%

Further characterization of the binding of heparin to granulocyte colony‐stimulating factor: Importance of sulfate groups

Liang

Liu

et al. 2008

Electrophoresis

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Heparin mediates fundamental biological mechanisms through interaction with proteins. Previously, we have shown that standard heparin binds to granulocyte colony-stimulating factor (G-CSF) with an affinity of 4.8 x 10(5) M(-1). To further study the structural features in heparin that are responsible for this interaction, we studied the bindings of G-CSF and N-desulfated and 2,3-O-desulfated heparin by CZE. Results showed that the N-desulfated heparin had a similar affinity for G-CSF ((5.4 +/- 0.9) x 10(5) M(-1)), but the 2,3-O-desulfated heparin had a 1000-fold lower affinity ((3.4 +/- 1.2) x 10(2) M(-1)) in comparison to standard heparin. The results showed that 2,3-O-sulfate groups are more important than N-sulfate groups in heparin-G-CSF interaction.

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Granulocyte-colony stimulating factor induced intranuclear endonuclease in murine leukemia cell line

Cited by 5 publications

References 29 publications

Structural features in carrageenan that interact with a heparin-binding hematopoietic growth factor and modulate its biological activity

Structural features in carrageenan that interact with a heparin-binding hematopoietic growth factor and modulate its biological activity

Combination regimen of granulocyte colony-stimulating factor and recombinant human thrombopoietin improves the curative effect on elderly patients with leukemia through inducing pyroptosis and ferroptosis of leukemia cells

Further characterization of the binding of heparin to granulocyte colony‐stimulating factor: Importance of sulfate groups

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