Granulocyte Colony-Stimulating Factor Facilitates the Angiogenesis Induced by Ultrasonic Microbubble Destruction

A targeted high-efficiency angiogenesis strategy as therapy for myocardial infarction

“…Capillary density in the HGF-US/MB group was significantly higher than in either the HGF/US-treated or HGF-treated groups. Studies in an ischemic hind limb model and cardiomyopathy model (Chappell et al, 2008;Miyake et al, 2007) have shown that US-mediated destruction of contrast MB significantly enhances the angiogenic response. Similarly, in the current experiments, we observed that HGF expression and microvessel counts were higher in the HGF-US/MB group than in either of the two other HGF treatment groups.…”

“…MB, used as a carrier, is mixed with DNA or drugs for delivery to the targeted areas (Eshet et al, 2006;Guo et al, 2006;Shohet et al, 2000). Ultrasonic destruction of MB in the microcirculation also causes local inflammatory cell infiltration, which has been shown to induce angiogenesis (Miyake et al, 2007).…”

“…Research efforts have therefore focused on local delivery of angiogenic factors at low doses (Shy et al, 2002). In addition, studies have demonstrated that US-mediated destruction of contrast MB stimulates angiogenesis (Miyake et al, 2007). There is also evidence that intramuscular implantation of HGF also induces angiogenesis.…”

Catheter-based transendocardial microbubble and pEGFP delivery combination of intracardiac ultrasonic exposure enhances EGFP gene expression

“…Combination with specific application techniques, such as intramyocardial injection, catheter-based delivery, ultrasound targeted microbubble destruction, may further enhance vector efficiency [1][2][3][4]. The goals of this study were as follows: a) evaluate the feasibility and safety of a catheter-based transendocardial injection of mixture of gene and microbubbles and intracardiac insonation in normal myocardium, b) determine whether in intramyocardial delivery the mixture of low-dosed pEGFP and microbubble combination of intracardiac insonation could further enhance EGFP expression.…”

“…As shown in Table 1, Compared with the EGFP group, the EGFP fluorescence intensity of EGFP + MB/US group was about 15.6 times higher (P b 0.01); and of the EGFP + US group, fluorescence intensity was about 7.0 times higher (P b 0.01). Despite many advances in diagnosis and therapy, heart failure (HF) is still apparently related to poor prognosis, with reported mortality rates at 5-year over 40% (9% per year) -a six-fold adverse rate compared with the general population [1][2][3]. To address this important public health issue, we sought to determine morbidity and mortality rates in our population of chronic HF out-patients, followed-up in the heart failure clinic of our Institution and compare them with rates reported in previous surveys.…”