Granulocyte and monocyte adsorptive apheresis induces apoptosis of neutrophils and release of a novel chemoattractant for desensitization of interleukin-8 response – In vitro and in vivo results

Kashiwagi, Nobuhito; Saito, Fumio; Maegawa, Hidetaka; Kaneda, Kenta

doi:10.1016/j.cyto.2020.155410

Cited by 5 publications

(3 citation statements)

References 38 publications

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“…Thus, improving intestinal lesions is considered to prevent liver impairment. After the introduction of GMA for the management of UC, studies identified various cytokines that are involved in its effects 18,24 . However, it remains unknown how GMA affects the pathophysiology of UC and PSC and TH17.…”

Section: Discussionmentioning

confidence: 99%

A retrospective study to investigate the efficacy and safety of granulocyte and monocyte adsorptive apheresis in patients with primary sclerosing cholangitis with ulcerative colitis

Ito,

Murasugi,

Yonezawa

et al. 2023

J of Clinical Apheresis

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Background and AimsPrimary sclerosing cholangitis has a poor prognosis and can be accompanied by ulcerative colitis. Infection control is essential, so immunosuppressive drugs should ideally be preferably. Granulocyte and monocyte adsorptive apheresis does not suppress the immune system and is used to treat ulcerative colitis. Therefore, this study investigated the efficacy and safety of granulocyte and monocyte adsorptive apheresis in patients with primary sclerosing cholangitis and ulcerative colitis.MethodsWe retrospectively evaluated data from patients with primary sclerosing cholangitis with ulcerative colitis who visited our hospital from April 2000 to December 2022 and underwent granulocyte and monocyte adsorptive apheresis (n = 10, number of treatment cycles = 15). Study endpoints were remission induction rate and safety, assessed as changes in liver functions and adverse events.ResultsSeven of the 10 patients were male. The median (min‐max) age was 23 (18‐77) years. The most common disease type was right‐dominant pancolitis. Remission occurred after 86.6% of cycles (13/15). Serum alkaline phosphatase and Aspartate transaminase were significantly lower after treatment (P = .0124, P = .002), and no negative effects on liver function were seen. The only adverse events were headache (n = 1) and decreased blood pressure (n = 1).ConclusionsGranulocyte and monocyte adsorptive apheresis has high efficacy for intestinal lesions and improves alkaline phosphatase and aspartate transaminase levels (high levels are a poor prognosis factor). It appears to be a treatment option in patients with primary sclerosing cholangitis associated with ulcerative colitis.

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Section: Discussionmentioning

confidence: 99%

A retrospective study to investigate the efficacy and safety of granulocyte and monocyte adsorptive apheresis in patients with primary sclerosing cholangitis with ulcerative colitis

Ito,

Murasugi,

Yonezawa

et al. 2023

J of Clinical Apheresis

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“…What’s more, AML12 cells may have adaptive immunomodulatory mechanisms that improve the response to CDs, leading to more apoptosis and cell viability than KUP5 cells in response to higher CDs doses. Here, adaptive immune regulation can be understood as AML12 cells may clear damaged cells by increasing apoptosis so that this cell type can maintain a certain level of cellular viability in an adverse environment [ 51 , 52 ]. Both liver cell types also showed high expression of the pro-apoptotic protein, Bax, and Cleaved caspase3, and low expression of the anti-apoptotic protein, Bcl-2.…”

Section: Discussionmentioning

confidence: 99%

TFEB-lysosome pathway activation is associated with different cell death responses to carbon quantum dots in Kupffer cells and hepatocytes

et al. 2022

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Background Carbon dot has been widely used in biomedical field as a kind of nanomaterial with low toxicity and high biocompatibility. CDs has demonstrated its unique advantages in assisted drug delivery, target diagnosis and targeted therapy with its small size and spontaneous fluorescence. However, the potential biosafety of CDs cannot be evaluated. Therefore, we focused on the study of liver, the target organ involved in CDs metabolism, to evaluate the risk of CDs in vitro. Methods and results Liver macrophage KUP5 cells and normal liver cells AML12 cells were incubated in CDs at the same concentration for 24 h to compare the different effects under the same exposure conditions. The study found that both liver cell models showed ATP metabolism disorder, membrane damage, autophagosome formation and lysosome damage, but the difference was that, KUP5 cells exhibited more serious damage than AML12 cells, suggesting that immunogenic cell type is particularly sensitive to CDs. The underlying mechanism of CDs-induced death of the two hepatocyte types were also assessed. In KUP5 cells, death was caused by inhibition of autophagic flux caused by autophagosome accumulation, this process that was reversed when autophagosome accumulation was prevented by 3-MA. AML12 cells had no such response, suggesting that the accumulation of autophagosomes caused by CDs may be specific to macrophages. Conclusion Activation of the TFEB-lysosome pathway is important in regulating autophagy and apoptosis. The dual regulation of ERK and mTOR phosphorylation upstream of TFEB influences the death outcome of AML12 cells. These findings provide a new understanding of how CDs impact different liver cells and contribute to a more complete toxicological safety evaluation of CDs.

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“…The removal of activated granulocyte, lymphocytes monocytes, and aggregated platelets by all types of AC results in downregulation of proinflammatory cytokines, IL8 included, and IFNγ, as well as upregulation of CD4+, CD25+, Foxp3+ T‐reg cells, lessening acute, and chronic inflammatory processes 24‐27 . The reduction of L‐selectin and other cell adhesion molecules following AC, affects chemotaxis and migration of granulocytes, monocytes, and leucocytes into the inflamed gut 28,29 …”

Section: Mechanisms Of Action Of Adsorptive Cytapheresismentioning

confidence: 99%

Adsorptive cytapheresis in ulcerative colitis: A non‐pharmacological therapeutic approach revisited

Vernia,

Viscido,

Latella

2023

J of Clinical Apheresis

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Adsorptive cytapheresis proves effective in a proportion of patients affected by ulcerative colitis. Relatively high cost and the need for apheresis facilities, prevented the widespread use of this therapeutic approach. More so following the introduction of anti‐TNFα biosimilars which proved both effective and inexpensive. Anti‐TNFα agents, however, are burdened by high rate of primary and secondary non‐response and prompt switching to new, high‐cost biologics, and small molecules. The present review analyzes advantages and disadvantages of adsorptive cytapheresis in the present clinical scenario and suggests its repositioning in the therapeutic workup of selected subgroups of ulcerative colitis patients. The extremely favorable safety profile makes adsorptive cytapheresis a viable therapeutic option in elderly and high‐risk UC patients, as well as potential second‐line treatment in corticosteroid‐dependent patients and poor responders to first‐line biologics.

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Granulocyte and monocyte adsorptive apheresis induces apoptosis of neutrophils and release of a novel chemoattractant for desensitization of interleukin-8 response – In vitro and in vivo results

Cited by 5 publications

References 38 publications

A retrospective study to investigate the efficacy and safety of granulocyte and monocyte adsorptive apheresis in patients with primary sclerosing cholangitis with ulcerative colitis

A retrospective study to investigate the efficacy and safety of granulocyte and monocyte adsorptive apheresis in patients with primary sclerosing cholangitis with ulcerative colitis

TFEB-lysosome pathway activation is associated with different cell death responses to carbon quantum dots in Kupffer cells and hepatocytes

Adsorptive cytapheresis in ulcerative colitis: A non‐pharmacological therapeutic approach revisited

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