Granulicatella bacteraemia in children: two cases and review of the literature

Luca, Maia De; Amodio, Donato; Chiurchiù, Sara; Castelluzzo, Maria Assunta; Rinelli, Gabriele; Bernaschi, Paola; Carducci, Francesca Ippolita Calò; D’Argenio, Patrizia

doi:10.1186/1471-2431-13-61

Cited by 15 publications

(15 citation statements)

References 17 publications

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“…The most notable differences observed between cases and controls was the enrichment for OTUs belonging to Granulicatella, Abiotrophia, and Streptococcus among the sputum of the cases. Interestingly, Granulicatella has been previously identified as part of the normal flora of the respiratory tract [Harris et al, ], and is being increasing implicated in clinically relevant infections leading to central nervous system infections, sinusitis, and other infections [Michelow et al, ; Hepburn et al, ; De Luca et al, ]. Abiotrophia has been associated with aortitis [Raff et al, ].…”

Section: Discussionmentioning

confidence: 99%

The potential role of lung microbiota in lung cancer attributed to household coal burning exposures

Hosgood

Sapkota

Rothman

et al. 2014

Environ and Mol Mutagen

172

139

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Bacteria influence site-specific disease etiology and the host’s ability to metabolize xenobiotics, such as polycyclic aromatic hydrocarbons (PAHs). Lung cancer in Xuanwei, China has been attributed to PAH-rich household air pollution from burning coal. This study seeks to explore the role of lung microbiota in lung cancer among never smoking Xuanwei women and how coal burning may influence these associations. DNA from sputum and buccal samples of never smoking lung cancer cases (n = 8, in duplicate) and controls (n = 8, in duplicate) in two Xuanwei villages was extracted using a multi-step enzymatic and physical lysis, followed by a standardized clean-up. V1–V2 regions of 16S rRNA genes were PCR-amplified. Purified amplicons were sequenced by 454 FLX Titanium pyrosequencing and high-quality sequences were evaluated for diversity and taxonomic membership. Bacterial diversity among cases and controls was similar in buccal samples (P = 0.46), but significantly different in sputum samples (P = 0.038). In sputum, Granulicatella (6.1 vs. 2.0%; P = 0.0016), Abiotrophia (1.5 vs. 0.085%; P = 0.0036), and Streptococcus (40.1 vs. 19.8%; P = 0.0142) were enriched in cases compared with controls. Sputum samples had on average 488.25 species-level OTUs in the flora of cases who used smoky coal (PAHrich) compared with 352.5 OTUs among cases who used smokeless coal (PAH-poor; P = 0.047). These differences were explained by the Bacilli species (Streptococcus infantis and Streptococcus anginosus). Our small study suggests that never smoking lung cancer cases have differing sputum microbiota than controls.

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Section: Discussionmentioning

confidence: 99%

The potential role of lung microbiota in lung cancer attributed to household coal burning exposures

Hosgood

Sapkota

Rothman

et al. 2014

Environ and Mol Mutagen

172

139

View full text Add to dashboard Cite

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“…A reduction in oral microbial diversity is generally associated with dysbiosis and a predisposition to disease 8 . In addition, the predominant genera of Granulicatella , Neisseria and Porphyromonas found after treatment with the Italian-style gluten-free diet all include species often associated with infection and disease 39 40 41 42 . Therefore, if the celiac children on an African-style gluten-free diet had imbalanced oral microbiota due to their disease state and diet, the westernisation of their diet apparently led to a different type of imbalance, favouring the growth of only a few microbial genera.…”

Section: Discussionmentioning

confidence: 99%

From an imbalance to a new imbalance: Italian-style gluten-free diet alters the salivary microbiota and metabolome of African celiac children

Ercolini

Francavilla

Vannini

et al. 2015

Sci Rep

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Fourteen Saharawi celiac children following an African-style gluten-free diet for at least two years were subjected to a change of diet to an Italian-style gluten-free diet for 60 days. Significant differences were identified in the salivary microbiota and metabolome when Saharawi celiac children switched from African- to Italian-style dietary habits. An Italian-style gluten-free diet caused increases in the abundance of Granulicatella, Porphyromonas and Neisseria and decreases in Clostridium, Prevotella and Veillonella, altering the ‘salivary type’ of the individuals. Furthermore, operational taxonomic unit co-occurrence/exclusion patterns indicated that the initial equilibrium of co-occurring microbial species was perturbed by a change in diet: the microbial diversity was reduced, with a few species out-competing the previously established microbiota and becoming dominant. Analysis of predicted metagenomes revealed a remarkable change in the metabolic potential of the microbiota following the diet change, with increased potential for amino acid, vitamin and co-factor metabolism. High concentrations of acetone and 2-butanone during treatment with the Italian-style gluten-free diet suggested metabolic dysfunction in the Saharawi celiac children. The findings of this study support the need for a translational medicine pipeline to examine interactions between food and microbiota when evaluating human development, nutritional needs and the impact and consequences of westernisation.

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“…Abiotrophia and Granulicatella species are very common inhabitants in human normal oral microbiota, in spite of their fastidiousness in growth [9,11,18,19,20], and are significant causative pathogens of endocarditis, bacteremia, and other systemic infections [21,22,23,24]. They often cannot grow on commercial blood agar plates used for the usual clinical examination, and even if they could grow on supplemented culture plates, their colonies are sometimes small, 0.2 to 0.5 mm in diameter [1,25].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Susceptibilities of Oral Isolates of Abiotrophia and Granulicatella According to the Consensus Guidelines for Fastidious Bacteria

2018

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Background: The genera Abiotrophia and Granulicatella, previously known as nutritionally variant streptococci (NVS), are fastidious bacteria requiring vitamin B6 analogs for growth. They are members of human normal oral microbiota, and are supposed to be one of the important pathogens for so-called “culture-negative” endocarditis. Methods: The type strains and oral isolates identified, by using both phenotypic profiles and the DNA–DNA hybridization method, were examined for susceptibilities to 15 antimicrobial agents including penicillin (benzylpenicillin, ampicillin, amoxicillin, and piperacillin), cephem (cefazolin, ceftazidime, ceftriaxone, and cefaclor), carbapenem (imipenem), aminoglycoside (gentamicin), macrolide (erythromycin), quinolone (ciprofloxacin), tetracycline (minocycline), glycopeptide (vancomycin), and trimethoprim-sulfamethoxazole complex. The minimum inhibitory concentration and susceptibility criterion were determined, according to the consensus guideline from the Clinical and Laboratory Standards Institute. Results: Isolates of Abiotrophia defectiva were susceptible to ampicillin, amoxicillin ceftriaxone, cefaclor, imipenem, ciprofloxacin, and vancomycin. Isolates of Granulicatella adiacens were mostly susceptible to benzylpenicillin, ampicillin, amoxicillin, cefazolin, ceftriaxone, imipenem, minocycline, and vancomycin. The susceptibility profile of Granulicatella elegans was similar to that of G. adiacens, and the susceptibility rate was higher than that of G. adiacens. Conclusions: Although Abiotrophia and Granulicatella strains are hardly distinguishable by their phenotypic characteristics, their susceptibility profiles to the antimicrobial agents were different among the species. Species-related differences in susceptibility of antibiotics should be considered in the clinical treatment for NVS related infections.

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Granulicatella bacteraemia in children: two cases and review of the literature

Cited by 15 publications

References 17 publications

The potential role of lung microbiota in lung cancer attributed to household coal burning exposures

The potential role of lung microbiota in lung cancer attributed to household coal burning exposures

From an imbalance to a new imbalance: Italian-style gluten-free diet alters the salivary microbiota and metabolome of African celiac children

Antimicrobial Susceptibilities of Oral Isolates of Abiotrophia and Granulicatella According to the Consensus Guidelines for Fastidious Bacteria

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