Granule cells in the infrapyramidal blade of the dentate gyrus are activated during paradoxical (REM) sleep hypersomnia but not during wakefulness: a study using TRAP mice

Abstract

Study Objectives Determine whether in the hippocampus and the supramammillary nucleus (SuM) the same neurons are reactivated when mice are exposed one week apart to two periods of wakefulness (W-W), paradoxical sleep rebound (PSR-PSR) or a period of W followed by a period of PSR (W-PSR) Methods We combined the innovative TRAP2 mice method in which neurons expressing cFos permanently express tdTomato after tamoxifen injection with cFos immunohistochemistry. Results We found out that a large number of tdTomato+ and cFos+ cells are localized in the dentate gyrus (DG) after PSR and W while CA1 and CA3 contained both types of neurons only after W. The number of cFos+ cells in the infrapyramidal but not the suprapyramidal blade of the DG was positively correlated with the amount of PS. In addition, we did not find double-labeled cells in the DG whatever the group of mice. In contrast, a high percentage of CA1 neurons were double-labeled in W-W mice. Finally, in the supramammillary nucleus, a large number of cells were double-labeled in W-W, PSR-PSR but not in W-PSR mice. Conclusions Altogether, our results are the first to show that different neurons are activated during W and PS in the supramammillary nucleus and the hippocampus. Further, we showed for the first time that granule cells of the infrapyramidal blade of the DG are activated during PS but not during W. Further experiments are now needed to determine whether these granule cells belong to memory engrams inducing memory reactivation during PS.

Sleep-dependent engram reactivation during hippocampal memory consolidation is associated with subregion-specific biosynthetic changes

“…Together, our data advance the findings of previous studies [17, 26, 28, 29, 51] highlighting the heterogeneity of hippocampal subregions’ function in the context of learning, and response to subsequent sleep or sleep loss. The present findings suggest that the influences of sleep and SD on hippocampally-mediated memory consolidation are linked to subregion-specific changes in engram neuron reactivation and biosynthetic events.…”

Reactivation of memory-encoding dentate gyrus neurons during memory consolidation is associated with subregion-specific, learning- and sleep-mediated biosynthetic changes

“…Together, our data advance the findings of previous studies [17,26,28,29,51] highlighting the heterogeneity of hippocampal subregions' function in the context of learning, and response to subsequent sleep or sleep loss. The present findings suggest that the influences of sleep and SD on hippocampally-mediated memory consolidation are linked to subregion-specific changes in engram neuron reactivation and biosynthetic events.…”

Reactivation of memory-encoding dentate gyrus neurons during memory consolidation is associated with subregion-specific, learning- and sleep-mediated biosynthetic changes

“…Together, our data advance the findings of previous studies [17,26,28,29,51] highlighting the heterogeneity of hippocampal subregions' function in the context of learning, and response to subsequent sleep or sleep loss. The present findings suggest that the influences of sleep and SD on hippocampally-mediated memory consolidation are linked to subregion-specific changes in engram neuron reactivation and biosynthetic events.…”