Granular hydrogels: emergent properties of jammed hydrogel microparticles and their applications in tissue repair and regeneration

Riley, Lindsay; Schirmer, Lucas; Segura, Tatiana

doi:10.1016/j.copbio.2018.11.001

Cited by 176 publications

(186 citation statements)

References 59 publications

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“…Prior studies and recent reviews have documented the strength and potential of microgels for tissue engineering ( 2, 13 ). While this method allows for great versatility, the spherical nature of microgels used in those approaches have certain limitations.…”

Section: Discussionmentioning

confidence: 99%

“…Going one step further, multimaterial approaches are the next step to introduce heterogeneity in microgel systems ( 2, 13 ). For entangled microstrands this is relevant at two different levels.…”

Section: Discussionmentioning

confidence: 99%

“…They can be ‘jammed’ into a close-pack state, and held together by weak particle-particle interactions which are disrupted during extrusion in the nozzle and reformed during the post-print phase ( 10 ). Simultaneously, due to the inherent void space between the microgels in a close-packed state, cells within these materials have enhanced viability, spreading and migration compared to bulk monolithic materials ( 12, 13 ).…”

Section: Introductionmentioning

confidence: 99%

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3D Bioprinting of Architected Hydrogels from Entangled Microstrands

Kessel¹,

Lee²,

Bonato³

et al. 2019

Preprint

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31Hydrogels are an excellent biomimetic of the extracellular matrix and have found great 32 use in tissue engineering. Nanoporous monolithic hydrogels have limited mass transport, 33 restricting diffusion of key biomolecules. Structured microbead-hydrogels overcome some 34 of these limitations, but suffer from lack of controlled anisotropy. Here we introduce a 35 novel method for producing architected hydrogels based on entanglement of microstrands. 36 The microstrands are mouldable and form a porous structure which is stable in water. 37 Entangled microstrands are useable as bioinks for 3D bioprinting, where they align during 38 the extrusion process. Cells co-printed with the microstrands show excellent viability and 39 augmented matrix deposition resulting in a modulus increase from 2.7 kPa to 780.2 kPa 40 after 6 weeks of culture. Entangled microstands are a new class of bioinks with 41 unprecedented advantages in terms of scalability, material versatility, mass transport, 42 showing foremost outstanding properties as a bioink for 3D printed tissue grafts. 43 44 45 46 47 48 49 50 129 130 131 Results 132 133 Entangled Microstrand Materials are Mouldable, Stable in Water and Macroporous 134 135Here we report on a robust and versatile method for preparing 'entangled' microstrands. 136 Bulk hyaluronan-methacrylate (HA-MA) hydrogels were mechanically pressed through a 137

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

3D Bioprinting of Architected Hydrogels from Entangled Microstrands

Kessel¹,

Lee²,

Bonato³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Granular hydrogels comprise an agglomeration of hydrogel microparticles in a packed state, where the packing can either be loose to retain porosity between particles or in a highly jammed state to restrict particle movement . The physical interactions between particles in granular systems lead to shear‐thinning behavior that permits injectability without introduction of chemical modification or secondary inter‐particle crosslinking to alter granular hydrogel properties.…”

Section: Physical Associations To Assemble Particle‐based Hydrogelsmentioning

confidence: 99%

Recent advances in shear‐thinning and self‐healing hydrogels for biomedical applications

Uman

Dhand

Burdick

2019

J of Applied Polymer Sci

243

215

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Shear‐thinning and self‐healing hydrogels are being investigated in various biomedical applications including drug delivery, tissue engineering, and 3D bioprinting. Such hydrogels are formed through dynamic and reversible interactions between polymers or polypeptides that allow these shear‐thinning and self‐healing properties, including physical associations (e.g., hydrogen bonds, guest–host interactions, biorecognition motifs, hydrophobicity, electrostatics, and metal–ligand coordination) and dynamic covalent chemistry (e.g., Schiff base, oxime chemistry, disulfide bonds, and reversible Diels–Alder). Their shear‐thinning properties allow for injectability, as the hydrogel exhibits viscous flow under shear, and their self‐healing nature allows for stabilization when shear is removed. Hydrogels can be formulated as uniform polymer and polypeptide assemblies, as hydrogel nanocomposites, or in granular hydrogel form. This review focuses on recent advances in shear‐thinning and self‐healing hydrogels that are promising for biomedical applications. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48668.

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“…The ability to design such scaffolds with hollow features enables loading of cargo with different compositions in controllable arrangements, as to fabricate scaffolds with spatially defined instructive cues. As a proof of principle, we employed 3D‐printed microcages loaded with microscale granular hydrogels (microgels) [ 7 ] supplemented with growth factors of varying compositions, [ 8 ] which show enhanced cell invasion into the core of assembled constructs in a controllable manner, thus accelerating the process of new tissue formation and healing.…”

Section: Figurementioning

confidence: 99%

3D Printing of Microgel‐Loaded Modular Microcages as Instructive Scaffolds for Tissue Engineering

et al. 2020

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Biomaterial scaffolds have served as the foundation of tissue engineering and regenerative medicine. However, scaffold systems are often difficult to scale in size or shape in order to fit defect‐specific dimensions, and thus provide only limited spatiotemporal control of therapeutic delivery and host tissue responses. Here, a lithography‐based 3D printing strategy is used to fabricate a novel miniaturized modular microcage scaffold system, which can be assembled and scaled manually with ease. Scalability is based on an intuitive concept of stacking modules, like conventional toy interlocking plastic blocks, allowing for literally thousands of potential geometric configurations, and without the need for specialized equipment. Moreover, the modular hollow‐microcage design allows each unit to be loaded with biologic cargo of different compositions, thus enabling controllable and easy patterning of therapeutics within the material in 3D. In summary, the concept of miniaturized microcage designs with such straight‐forward assembly and scalability, as well as controllable loading properties, is a flexible platform that can be extended to a wide range of materials for improved biological performance.

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Granular hydrogels: emergent properties of jammed hydrogel microparticles and their applications in tissue repair and regeneration

Cited by 176 publications

References 59 publications

3D Bioprinting of Architected Hydrogels from Entangled Microstrands

3D Bioprinting of Architected Hydrogels from Entangled Microstrands

Recent advances in shear‐thinning and self‐healing hydrogels for biomedical applications

3D Printing of Microgel‐Loaded Modular Microcages as Instructive Scaffolds for Tissue Engineering

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