Granatin B and punicalagin from Chinese herbal medicine pomegranate peels elicit reactive oxygen species-mediated apoptosis and cell cycle arrest in colorectal cancer cells

Chen, Xiaoxin; Khyeam, Sheamin; Zhang, Zhang-Jin; Zhang, Kalin Yanbo

doi:10.1016/j.phymed.2022.153923

Cited by 10 publications

(5 citation statements)

References 31 publications

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“…As a conventional anti-inflammation and anticancer TTM drug, LWMX pills comprise six Tibetan herbal medications that have anti-CRC potential. Specifically, numerous studies have indicated that Punica granatum exhibits strong anti-CRC therapeutic ability [ 22 , 23 , 24 ]. Additionally, Phyllanthi Fructus has been reported to have the potential to protect normal human colon epithelial NCM460 cells from mitotic aberrations and genomic instability partially by regulating the spindle assembly checkpoint [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Potential Mechanism of Tibetan Medicine Liuwei Muxiang Pills against Colorectal Cancer: Network Pharmacology and Bioinformatics Analyses

Qi,

Liang,

Wang

et al. 2024

Pharmaceuticals

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This study aimed to explore the mechanism through which Tibetan medicine Liuwei Muxiang (LWMX) pills acts against colorectal cancer (CRC). We firstly retrieved the active ingredients and the correlated targets of LWMX pills from public databases. The CRC-related targets were determined through bioinformatic analysis of a public CRC dataset. By computing the intersection of the drug-specific and disease-related targets, LWMX pill–CRC interaction networks were constructed using the protein–protein interaction (PPI) method and functional enrichment analysis. Subsequently, we determined the hub genes using machine learning tools and further verified their critical roles in CRC treatment via immune infiltration analysis and molecular docking studies. We identified 81 active ingredients in LWMX pills with 614 correlated targets, 1877 differentially expressed genes, and 9534 coexpression module genes related to CRC. A total of 5 target hub genes were identified among the 108 intersecting genes using machine learning algorithms. The immune infiltration analysis results suggested that LWMX pills could affect the CRC immune infiltration microenvironment by regulating the expression of the target hub genes. Finally, the molecular docking outcomes revealed stable binding affinity between all target hub proteins and the primary active ingredients of LWMX pills. Our findings illustrate the anti-CRC potential and the mechanism of action of LWMX pills and provide novel insights into multitarget medication for CRC treatment.

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Section: Discussionmentioning

confidence: 99%

Potential Mechanism of Tibetan Medicine Liuwei Muxiang Pills against Colorectal Cancer: Network Pharmacology and Bioinformatics Analyses

Qi,

Liang,

Wang

et al. 2024

Pharmaceuticals

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“…For example, bixin, an apocarotenoid from the seeds of Bixa orellana , was showed to attenuate the proliferation and motility of CRC cell lines (CaCO2 and SW480), followed by inhibiting CRC progression 18 . Granatin B and punicalagin, extracted from Chinese herbal medicine pomegranate peels, were also reported to induce cell apoptosis of CRC cells 19 . A recent study revealed that a large precious medicinal fungus, Sanghuang, also called Sanghuangporus vaninii , could lead to significant cell apoptosis of CRC cells through activating the intrinsic apoptotic pathway 20 .…”

Section: Discussionmentioning

confidence: 99%

Cincumol prevents malignant phenotype of colorectal cancer cell line HCT116 via inhibiting PI3K/AKT signaling in vitro

Chen

Peng

et al. 2022

Acta Cir. Bras.

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Purpose: Colorectal cancer (CRC) is a common human cancer along with higher incidence and mortality, and this study aimed to identify the effect of cincumol on CRC and its potential mechanisms. Methods: CRC cell line HCT116 was used as the material. Cell proliferation was evaluated by CCK-8 assay, and cell migration was detected by scratch test and Transwell assay. TUNEL staining assay was used to evaluate cell apoptosis. The expression of target genes was detected by qualitative real-time polymerase chain reaction and western blot assays. Results: Cincumol significantly reduced the proliferative and migratory rate and enhanced apoptotic rate of HCT116 cells. Meanwhile, the elevated levels of RBUsuh, Nicd and Tace was also observed in cincumol-treated HCT116 cells. Moreover, our findings revealed that additional cincumol inhibited the expression of p-PI3K and p-AKT, suggesting the inhibition of PI3K/AKT signaling might be involved in the protective role of cincumol on the malignant phenotypes of CRC cells in vitro . Conclusions: Cincumol inhibited the malignant phenotypes of CRC cells in vitro through inactivating PI3K/AKT signaling, suggesting that cincumol might be a potential anti-CRC agent.

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“…In fact, in HT-29 cell lines, PJ reduced COX-2 expression much more effectively than punicalagin, due to the synergistic effect of anthocyanins and flavonols [128]. It has also been demonstrated that punicalin, punicalagin, and granatin B isolated from pomegranate peels reduced HT-29 cells survival by 61.9 ± 4.1%, 86.2 ± 2.2% and 65.3 ± 2.4%, respectively, whereas treatment with granatin B and punicalagin induced S-phase cell cycle arrest and stimulated apoptosis pathways [129]. Pomegranate peel extract has, also, been encapsulated in AgNPs and its activity against colorectal cancer was investigated.…”

Section: Colon Cancermentioning

confidence: 99%

The Therapeutic Alliance between Pomegranate and Health Emphasizing on Anticancer Properties

et al. 2023

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Pomegranate is a fruit bearing-plant that is well known for its medicinal properties. Pomegranate is a good source of phenolic acids, tannins, and flavonoids. Pomegranate juice and by-products have attracted the scientific interest due to their potential health benefits. Currently, the medical community has showed great interest in exploiting pomegranate potential as a protective agent against several human diseases including cancer. This is demonstrated by the fact that there are more than 800 reports in the literature reporting pomegranate’s anticancer properties. This review is an update on the research outcomes of pomegranate’s potential against different types of human diseases, emphasizing on cancer. In addition, perspectives of potential applications of pomegranate, as a natural additive aiming to improve the quality of animal products, are discussed.

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Granatin B and punicalagin from Chinese herbal medicine pomegranate peels elicit reactive oxygen species-mediated apoptosis and cell cycle arrest in colorectal cancer cells

Cited by 10 publications

References 31 publications

Potential Mechanism of Tibetan Medicine Liuwei Muxiang Pills against Colorectal Cancer: Network Pharmacology and Bioinformatics Analyses

Potential Mechanism of Tibetan Medicine Liuwei Muxiang Pills against Colorectal Cancer: Network Pharmacology and Bioinformatics Analyses

Cincumol prevents malignant phenotype of colorectal cancer cell line HCT116 via inhibiting PI3K/AKT signaling in vitro

The Therapeutic Alliance between Pomegranate and Health Emphasizing on Anticancer Properties

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